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Genetic Insert: Para - cfp-parB
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Proper citation: RRID:Addgene_128305 Copy
Genetic Insert: Para - GCN4zip.MSE-cfp-parB
Vector Backbone Description: Vector Backbone:p15A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31406375
Comments: Cloning method: Golden Gate.
Proper citation: RRID:Addgene_128304 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf156
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12844 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf152
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12840 Copy
Species: Synthetic
Genetic Insert: Neomycin (NEO)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 7 sequence (CNAG_05557 - CNAG_05558) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128376 Copy
Species: Synthetic
Genetic Insert: Neomycin (NEO)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 6 sequence (CNAG_04586 - CNAG_04585) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128375 Copy
Species: Synthetic
Genetic Insert: Neomycin (NEO)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128374 Copy
Species: Synthetic
Genetic Insert: Hygromycin (HYG)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128379 Copy
Species: Staphylococcus simulans
Genetic Insert: Lysostaphin
Vector Backbone Description: Backbone Size:7499; Vector Backbone:pLG320; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792444
Proper citation: RRID:Addgene_128412 Copy
Species: Synthetic
Genetic Insert: Hygromycin (HYG)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128378 Copy
Species: Synthetic
Genetic Insert: Hygromycin (HYG)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128377 Copy
Species: Synthetic
Genetic Insert: gfpmut2
Vector Backbone Description: Vector Backbone:pLG166; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31792444
Proper citation: RRID:Addgene_128410 Copy
Species: Caenorhabditis elegans
Genetic Insert: vab-1
Vector Backbone Description: Backbone Size:3400; Vector Backbone:pSL1190; Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9506518
Proper citation: RRID:Addgene_128414 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf160
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12848 Copy
Species: Vaccinia Virus
Genetic Insert: VV.Orf157
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5514; Vector Backbone:pcDNA 3.1D V5-His-TOPO; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15623576
Comments: Please see the Vaccinia kit page ( https://www.addgene.org/vvorf/ ) for more information. The kit page contains the supplemental VVOrfData spreadsheet, which includes important information about this plasmid. The plasmid and insert names provided here relate only to this library. The name of the ORF (if present) in VACV strain Copenhagen and the name of the ORF (if annotated) in VACV strain WR sequence can be found in VVOrfData spreadsheet. Please note that the amino acid sequence given for each ORF in the spreadsheet may contain some errors. Additionally, changes from the expected sequence may be present in these plasmids. Please see Addgene's quality control sequence and sequencing notes in the VVOrfData spreadsheet before ordering this plasmid.
Proper citation: RRID:Addgene_12845 Copy
Species: Homo sapiens
Genetic Insert: C9ORF80
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5369; Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29150435
Proper citation: RRID:Addgene_128418 Copy
Species: Synthetic
Genetic Insert: Neomycin (NEO)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 3 sequence (CNAG_02589 - CNAG_02588) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128372 Copy
Species: Synthetic
Genetic Insert: Acetamidase (amdS)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 5 sequence (CNAG_05670 - CNAG_05671) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128364 Copy
Species: Synthetic
Genetic Insert: Noureothricin (NAT)
Vector Backbone Description: Backbone Marker:Stratagene; Vector Backbone:pBlueScript II SK(-); Vector Types:Unspecified; Bacterial Resistance:Ampicillin
Defining Citation: PMID:31220607
Comments: Contains the Safe Haven Site 4 sequence (CNAG_06526 - CNAG_06527) in the backbone of pBluescript II SK- to enable integration into the C. neoformans genome via homologous recombination, in addition to a linker sequence containing multiple rare-cutting restriction enzyme cut sites to facilitate linearization prior to biolistic transformation. Importantly, as the Safe Haven and marker fragments were inserted into the pBluescript II SK- backbone in place of the f1 origin of replication, each plasmid still retains the original multicloning site and blue/white screening capabilities. Linearization of the safe haven constructs is made possible by the inclusion of a linker containing cut sites for three rare cutting restriction enzymes, BaeI, AscI and PacI. To use the vectors, the gene of interest is subcloned into the standard pBluescript II SK- multicloning site.
Proper citation: RRID:Addgene_128368 Copy
Species: C. tetani
Genetic Insert: Tetanus toxin fragment C domain
Vector Backbone Description: Backbone Size:4535; Vector Backbone:pTrc99S; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:33536221
Proper citation: RRID:Addgene_128401 Copy
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