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Species: Arabidopsis thaliana
Genetic Insert: PhyB
Vector Backbone Description: Backbone Size:7236; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23761071
Comments: There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.
Proper citation: RRID:Addgene_51568 Copy
Species: Arabidopsis thaliana
Genetic Insert: PhyB
Vector Backbone Description: Backbone Size:7236; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23761071
Comments: There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.
Proper citation: RRID:Addgene_51571 Copy
Species: Arabidopsis thaliana
Genetic Insert: PIF6
Vector Backbone Description: Backbone Size:3657; Vector Backbone:F6A-NAT; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23761071
Comments: the 11-aa linker is AAAGDGAGLIN
Proper citation: RRID:Addgene_51576 Copy
Species: Arabidopsis thaliana
Genetic Insert: PhyB
Vector Backbone Description: Backbone Size:7236; Vector Backbone:pRS305; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23761071
Comments: There is a 15-aa linker (EFDSAGSAGSAGGSS) between the PhyB and mCherry and a 10-aa linker (SAGSAGKASG) between mCherry and anchor gene.
Proper citation: RRID:Addgene_51582 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51778 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51775 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51774 Copy
Species: Arabidopsis thaliana
Genetic Insert: guide RNA targeting AtPDS3
Vector Backbone Description: Backbone Size:3162; Vector Backbone:pUC119; Vector Types:CRISPR, Plant expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23929339
Comments: gRNA target sequence GGACTTTTGCCAGCCATGGT
This plasmid is used as a PCR template to assemble new desired guide RNA according to the strategy published in Figure S5 of our Nature Biotechnology paper.
Proper citation: RRID:Addgene_52255 Copy
Species: Arabidopsis thaliana
Genetic Insert: zep
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:10040; Vector Backbone:pAC-ZEAXipi; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24506237
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53087 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac-100
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47771 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47770 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac-GS
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47769 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac1;2
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47766 Copy
Species: Arabidopsis thaliana
Genetic Insert: AmTrac-LS
Vector Backbone Description: Backbone Size:6000; Vector Backbone:pDR GW; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23840931
Proper citation: RRID:Addgene_47767 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyE
Vector Backbone Description: Backbone Marker:Francis X. Cunningham, Jr.; Backbone Size:9476; Vector Backbone:pAC-LYC; Vector Types:low copy number bacterial cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:8798688
Comments: For better yield of low copy number pAC-based plasmids, grow liquid cultures on a platform shaker at ca. 30 degrees Celsius. When cultures reach early stationary phase, dilute 2-fold with growth medium, add spectinomycin (150 mg/liter), and "amplify" for several hours before harvest. For plasmid selection and maintenance in E. coli, use chloramphenicol at 30 mg/liter.
Proper citation: RRID:Addgene_53273 Copy
Species: Arabidopsis thaliana
Genetic Insert: chyB1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4414; Vector Backbone:pTrcHis A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:15659105
Proper citation: RRID:Addgene_53367 Copy
Species: Arabidopsis thaliana
Genetic Insert: UBQ10 promoter
Vector Backbone Description: Backbone Marker:Mark Curtis at ETH Zurich (Brand et al., Plant Physiology 2006); Vector Backbone:pLB12; Vector Types:Other, plant expression; Bacterial Resistance:Spectinomycin
Comments: The vector pLB12 contains both an activator unit and a b-glucuronidase (GUS) reporter within a responder unit.
Proper citation: RRID:Addgene_53449 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyB
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK-; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17085635
Proper citation: RRID:Addgene_53588 Copy
Species: Arabidopsis thaliana
Genetic Insert: lcyE
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:2958; Vector Backbone:pBluescript SK-; Vector Types:; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8837512
Proper citation: RRID:Addgene_53586 Copy
Species: Arabidopsis thaliana
Genetic Insert: Cry2
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5428; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24920824
Proper citation: RRID:Addgene_64208 Copy
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