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Vector Backbone Description: Backbone Marker:Ghader Bashiri; Backbone Size:5087; Vector Backbone:pYUB1049; Vector Types:Bacterial Expression, mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21209917
Proper citation: RRID:Addgene_37278 Copy
Vector Backbone Description: Backbone Size:4203; Vector Backbone:pVV16, pGOAL17; Vector Types:Bacterial Expression, E.coli-mycobacteria shuttle vector; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23315736
Proper citation: RRID:Addgene_44566 Copy
Vector Backbone Description: Backbone Marker:Daugelat et al 2003; Backbone Size:6900; Vector Backbone:pSD26; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33006405
Comments: Vector constructed by Dr. Arie Geerlof at EMBL-Hamburg (now at Helmholtz Zentrum München)
Proper citation: RRID:Addgene_42192 Copy
Species: Influenza A/FPV/Rostock/1934, subtype H7
Genetic Insert: hemagglutinin gene
Vector Backbone Description: Backbone Marker:InvivoGen; Backbone Size:6321; Vector Backbone:pVITRO2-mcs; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:23319179
Comments: Please note that a T55A mutation was identified in the N1 sequence.
Proper citation: RRID:Addgene_44169 Copy
Genetic Insert: I-SceI
Vector Backbone Description: Vector Backbone:pmsg383; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:21219454
Proper citation: RRID:Addgene_48789 Copy
Species: Other
Genetic Insert: T38M-P750-luxAB-DAS+4
Vector Backbone Description: Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24191058
Proper citation: RRID:Addgene_49520 Copy
Species: Other
Genetic Insert: P750-luxAB-DAS+4
Vector Backbone Description: Backbone Size:6465; Vector Backbone:pDE43-MEH; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:24191058
Proper citation: RRID:Addgene_49519 Copy
Species: Synthetic
Genetic Insert: mEos3.2_H-NSdbd
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_168774 Copy
Species: Synthetic
Genetic Insert: mEos3.2
Vector Backbone Description: Backbone Size:5625; Vector Backbone:pFPV27; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:34734265
Proper citation: RRID:Addgene_168775 Copy
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pGIlesSHdI; Vector Types:Unspecified; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_170048 Copy
Genetic Insert: mScarlet
Vector Backbone Description: Vector Backbone:pGilesSHdI; Vector Types:Unspecified; Bacterial Resistance:Hygromycin
Defining Citation: PMID:33362741
Proper citation: RRID:Addgene_170047 Copy
Genetic Insert: pmyc1tetO-lacZ, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: Expresses lacZ under control of tetR(B). Also see Pubmed ID 15687379.
Derivative of pMS2 with lacZ under the control of Pmyc1tetO and tetR under the control of Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17971 Copy
Genetic Insert: tetR(B)r1.7
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: revTetR is expressed from a strong mycobacterial promoter (psmyc = puv15). Also see Pubmed ID 18059281.
Derivative of pMS2 with tetR r1.7 expressed by Psmyc. pMS2 is a shuttle vector containing origins of replication for E. coli (pMB1) and mycobacteria (pAL500) and a multiple cloning site flanked by two transcriptional terminators.
Proper citation: RRID:Addgene_17973 Copy
Genetic Insert: pmyc1tetO-gfp, tetR(B)
Vector Backbone Description: Backbone Size:0; Vector Backbone:pMS2; Vector Types:mycobacteria expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:17483222
Comments: Expresses GFP under control of tetR(B). Also see Pubmed IDs 18059281 and 15687379.
pUV15 derivative, Genotype: Hygr, Kmr, pimyc-tetR, pmyc1 tetO-gfp+
Proper citation: RRID:Addgene_17975 Copy
Species: Synthetic
Genetic Insert: Fab_Quin-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119049 Copy
Species: Synthetic
Genetic Insert: Fab_MTX-LUCID_part1
Vector Backbone Description: Backbone Marker:Invivogen; Backbone Size:6295; Vector Backbone:pVITRO1-Hygro; Vector Types:Mammalian Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:28510347
Proper citation: RRID:Addgene_119047 Copy
Genetic Insert: BbaJ23104_EYFP
Vector Backbone Description: Vector Backbone:p15a; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:29366319
Proper citation: RRID:Addgene_119885 Copy
Vector Backbone Description: Vector Backbone:pML2424; Vector Types:Deletion generation; Bacterial Resistance:Hygromycin
Defining Citation: PMID:22885758
Comments: Addgene QC Next-generation sequencing identified sequence discrepancies relative to the Genbank file linked under Supplemental Documents. Plasmid is expected to function as described in the associated publication.
Proper citation: RRID:Addgene_117497 Copy
Vector Backbone Description: Backbone Marker:pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein; Backbone Size:5800; Vector Backbone:pYUB1062; Vector Types:Bacterial Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30319771
Comments: This is a shuttle vector, designed for expression of the inserted gene in Mycobacterium smegmatis as well as allowing cloning and expressions using E. coli.
This plasmid was constructed by Anajana Radhakrishnan from the pYUB1062 vector. pYUB1062 was donated by William R. Jacobs from the Department of Microbiology and Immunology at the Albert Einstein College of Medicine, New York, USA.
References: this paper has been accepted in RSC Advances:
A GFP-strategy for efficient recombinant protein overexpression and purification in Mycobacterium smegmatis: Anjana Radhakrishnan, Christopher M Furze, Mohd Syed Ahangar, Elizabeth Fullam
Proper citation: RRID:Addgene_117695 Copy
Vector Backbone Description: Backbone Size:4649; Vector Backbone:pMEG_BB3_YL18H_AC; Vector Types:Bacterial Expression, Yeast Expression; Bacterial Resistance:Hygromycin
Defining Citation: PMID:30698703
Comments: Contains 34 bp GoldenMOCS-Yali Linker AB
Proper citation: RRID:Addgene_117828 Copy
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