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Species: S. mediterranea
Genetic Insert: Protein arginine methyltransferase 1 homolog
Vector Backbone Description: Backbone Marker:Jim Collins, Newmark lab; Vector Backbone:pJC53.2; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22318224
Comments: There are several mismatches between Addgene's quality control sequence and the available NCBI sequence. Depositing lab believes these mismatches will not affect plasmid function.
Proper citation: RRID:Addgene_35694 Copy
Species: S. mediterranea
Genetic Insert: Protein arginine methyltransferase 5
Vector Backbone Description: Backbone Marker:Jim Collins, Newmark lab; Vector Backbone:pJC53.2; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:22318224
Comments: There are several mismatches between Addgene's quality control sequence and the best match available NCBI sequence (AEX33822.1). Depositing lab believes these mismatches will not affect plasmid function.
Proper citation: RRID:Addgene_35692 Copy
Species: Burkholderia xenovorans
Genetic Insert: coxM1′::lacZ reporter system with higher-affinity RcoM1 binding site at the "e" position AND RcoM1 with C-term 6xHIS
Vector Backbone Description: Vector Backbone:pUX3242 and pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3242 (pUX2996 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – higher-affinity binding site at the “e” position (“e↑” binding site = 5′-TCCTACAGTTCACGCACGT-3′) – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
The strain is CmR, pUX3242 is KanR and SpecR and pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37177 Copy
Genetic Insert: coxM1′::lacZ reporter system with RcoM binding sites "a-f" spaced at 21bp AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3254 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3254 (coxM1′::lacZ reporter system with binding sites “a-f” with a deletion between the “c” and “d” binding motifs such that the individual binding motifs “a,” “b,” “c,” “d,” “e,” and “f” are spaced at 21-bp intervals) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3254 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37178 Copy
Genetic Insert: coxM1′::lacZ reporter system of pUX3009 with an improved −35/ −10 RNAP binding region AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3272 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3272 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” and an improved −35/−10 RNAP binding region (5′-TTGACA-(18N)-TATCCT-3′) ) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3272 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37179 Copy
Genetic Insert: coxM1′::lacZ reporter system of pUX3009 with “e” binding site “TTnnnG” motif changed to “GGnnnG” AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3297 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3297 (pUX3009 based coxM1′::lacZ reporter system with binding sites “d + e + f” with the “e” binding site “TTnnnG” motif changed to “GGnnnG;”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3297 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37180 Copy
Genetic Insert: coxM1′::lacZ reporter system with RcoM binding site "f" AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3008 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3008 (coxM1′::lacZ reporter system with binding site “f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3008 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37169 Copy
Genetic Insert: coxM1′::lacZ reporter system AND Burkholderia xenovorans RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX2996 and pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX2996 (pUX2970 with a coxM1′::lacZ reporter system such that ...vector DNA – BamHI (unique site) – “e + f” binding region – coxM1′(with unique SalI site)::lacZ-> tfd <-KanR (with 5′-end unique XhoI site) – vector DNA <-SpR...) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, plasmid pUX2996 is KanR and SpecR and plasmid is pUX2410 is AmpR. Can be grown in media containing Amp + Kan.
For strain usage see attached pdf. Please note that this strain has not been tested functionally by Addgene.
Proper citation: RRID:Addgene_37167 Copy
Species: Burkholderia xenovorans
Genetic Insert: ~1200-bp fragment from Burkholderia xenovorans LB400 cloned into pEXT20
Vector Backbone Description: Vector Backbone:pUX2442; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:18326575
Comments: This strain contains Roberts lab plasmid pUX2442 which contains ~1200bp fragment from Burkholderia xenovorans LB400 with introduced terminal HindIII and SalI sites [such that HindIII – (<-rcoM1
– intergenic region – coxM1′->) – SalI] cloned into pEXT20 with the lacZ + KanR cassette from pKOK6 cloned at the SalI site such that coxM1′::lacZ->tfd<-KanR. Suitable template for amplification of the Burkholderia xenovorans RcoM1 binding region + reporter cassette.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37164 Copy
Genetic Insert: coxM1′::lacZ reporter system with RcoM binding sites "d + e + f" AND RcoM1 with C-term 6xHIS tag
Vector Backbone Description: Vector Backbone:pUX3009 AND pUX2410; Vector Types:; Bacterial Resistance:Ampicillin and Kanamycin
Comments: This is Roberts lab strain UQ5853 (Addgene #37159) containing Roberts lab plasmids pUX3009 (coxM1′::lacZ reporter system with binding sites “d + e + f”) AND pUX2410 (for expression of Burkholderia xenovorans RcoM1 protein + C-terminal 6×His tag).
Strain is CmR, pUX3009 is KanR and SpecR, pUX2410 is AmpR. Can be grown in Amp + Kan.
For strain usage see attached pdf.
Proper citation: RRID:Addgene_37171 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-dmd-4, 3’ RACE product
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23652002
Proper citation: RRID:Addgene_44692 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-dmd-1, 3’ RACE partial product
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23652002
Proper citation: RRID:Addgene_44689 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-dmd-1, full ORF, spice form 4
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23652002
Proper citation: RRID:Addgene_44688 Copy
Species: Schmidtea mediterranea
Genetic Insert: Smed-dmd-1, full ORF, spice form 1
Vector Backbone Description: Backbone Size:3240; Vector Backbone:pJC53.2; Vector Types:T/A Cloning Vector for dsRNA generation and the generation of sense and anti-sense probes; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23652002
Comments: The Addgene QC sequencing was unable to verify bp 1-14 of the depositor sequence.
Proper citation: RRID:Addgene_45028 Copy
Species: Mus musculus
Genetic Insert: alpha Klotho
Vector Backbone Description: Backbone Size:5710; Vector Backbone:pCR3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:26881702
Proper citation: RRID:Addgene_45532 Copy
Genetic Insert: FRT-kan-FRT
Vector Backbone Description: Backbone Marker:M. Koob (University of Wisconsin, Madison); Vector Backbone:pANTSγ; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:10829079
Comments: This is a template plasmids for frt-flanked kan cassette. The kan cassette originally came from pCP15.
pKD3 and pKD4 are identical except for the region between the FRT sites, so they create an identical scar that has stop codons in all six reading frames. As drawn in the map, this scar has an idealized ribosome binding site and start codon for downstream (rightward) gene expression.
Proper citation: RRID:Addgene_45605 Copy
Species: Synthetic
Genetic Insert: I-AAAT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42966 Copy
Species: Synthetic
Genetic Insert: I-AACA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_42967 Copy
Species: Synthetic
Genetic Insert: I-CGCG
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43065 Copy
Species: Synthetic
Genetic Insert: I-CGCT
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:3973; Vector Backbone:pCRII-TOPO; Vector Types:Bacterial Cloning; Bacterial Resistance:Ampicillin and Kanamycin
Defining Citation: PMID:23246482
Proper citation: RRID:Addgene_43066 Copy
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