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Species: Saccharomyces cerevisiae
Genetic Insert: Sgd1
Vector Backbone Description: Vector Backbone:pDONR221; Vector Types:Yeast Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29054886
Comments: Addgene NGS results found T307A, S332F, and V832F in the Sgd1 translation compared to the best-match NCBI reference sequence (GAA25198.1).
Proper citation: RRID:Addgene_100000 Copy
Species: Homo sapiens
Genetic Insert: 14-3-3 sigma human
Vector Backbone Description: Backbone Size:5369; Vector Backbone:pET28-his-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28931924
Comments: The 3C site was introduced immediately prior to 14-3-3 sequence to facilitate purification by subtractive IMAC.
Proper citation: RRID:Addgene_100093 Copy
Species: Homo sapiens
Genetic Insert: Fetal microtubule-associated protein tau
Vector Backbone Description: Backbone Size:5231; Vector Backbone:pET28-his-3C; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28575131
Comments: The 3C site is introduced in front of Tau sequence for easy purification by subtractive IMAC
Proper citation: RRID:Addgene_100092 Copy
Species: Homo sapiens
Genetic Insert: Pickles-2.34NES
Vector Backbone Description: Vector Backbone:pFX; Vector Types:Mammalian Expression, pFX vector is derived from pCMV vector.; Bacterial Resistance:Kanamycin
Defining Citation: PMID:27928132
Proper citation: RRID:Addgene_100028 Copy
Species: Neisseria wadsworthii
Genetic Insert: Concentrative nucleoside transporter
Vector Backbone Description: Vector Backbone:pET26; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28424521
Proper citation: RRID:Addgene_100164 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 13 of EMMA platform.
Proper citation: RRID:Addgene_100623 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 10 of EMMA platform.
Proper citation: RRID:Addgene_100620 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 1 of EMMA platform.
Proper citation: RRID:Addgene_100609 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 8b of EMMA platform.
Proper citation: RRID:Addgene_100618 Copy
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 3 of EMMA platform.
Proper citation: RRID:Addgene_100611 Copy
Species: Synthetic
Genetic Insert: tLOV
Vector Backbone Description: Backbone Size:3712; Vector Backbone:pTKEI-Dest; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28707884
Proper citation: RRID:Addgene_100574 Copy
Genetic Insert: AAVS1 sgRNA
Vector Backbone Description: Vector Backbone:gRNA_cloning_vector (#41824); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100554 Copy
Genetic Insert: EMX1 sgRNA
Vector Backbone Description: Vector Backbone:gRNA_cloning_vector (#41824); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100558 Copy
Genetic Insert: MYC sgRNA5
Vector Backbone Description: Vector Backbone:gRNA_cloning_vector (#41824); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100557 Copy
Genetic Insert: MYC sgRNA4
Vector Backbone Description: Vector Backbone:gRNA_cloning_vector (#41824); Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28737741
Proper citation: RRID:Addgene_100556 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector H-J
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 7 and 10 of EMMA platform.
Proper citation: RRID:Addgene_100647 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector Ha-K
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 8a and 11 of EMMA platform.
Proper citation: RRID:Addgene_100649 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector B-L
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 1 and 12 of EMMA platform.
Proper citation: RRID:Addgene_100645 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector A-C
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to fill in the gap between the backbone and parts placed in position 3 of EMMA platform.
Proper citation: RRID:Addgene_100640 Copy
Species: Synthetic
Genetic Insert: EMMA toolkit Assembly connector N-R
Vector Backbone Description: Vector Backbone:pSMART HC_Kan; Vector Types:Synthetic Biology; Bacterial Resistance:Kanamycin
Defining Citation: PMID:28418644
Comments: DNA element with no function. It is used as a bridge to connect parts placed in position 13 and 18 of EMMA platform.
Proper citation: RRID:Addgene_100658 Copy
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