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Genetic Insert: E. coli Conjugation genes
Vector Backbone Description: Vector Backbone:RK24; Vector Types:; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25188631
Comments: LB Lennox with Tetracycline to maintain plasmid, at 32°C or below. Strain has a biotin auxotrophy, but this should not affect growth in rich media.
Proper citation: RRID:Addgene_51950 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:9092628
Comments: AUG --> UUG start codon in lacZ
Proper citation: RRID:Addgene_63861 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a -1 frameshift mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63875 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63870 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a UAG nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63871 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63872 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:8415679
Comments: This plasmid carries a UAA nonsense mutation near the 5' end of lacZ.
Proper citation: RRID:Addgene_63873 Copy
Species: Escherichia coli
Genetic Insert: lacZ
Vector Backbone Description: Backbone Size:4500; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:9092628
Comments: AUG --> GUG start codon in lacZ
Proper citation: RRID:Addgene_63866 Copy
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Proper citation: RRID:Addgene_59399 Copy
Vector Backbone Description: Backbone Marker:Erb Lab; Backbone Size:6558; Vector Backbone:pTE100; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Tetracycline
Defining Citation: PMID:25105793
Proper citation: RRID:Addgene_59398 Copy
Genetic Insert: rfp
Vector Backbone Description: Backbone Size:7569; Vector Backbone:pCM62; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:24219429
Proper citation: RRID:Addgene_59775 Copy
Species: Synthetic
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pFG018; Vector Types:Bacterial Expression, CRISPR, Synthetic Biology; Bacterial Resistance:Tetracycline
Proper citation: RRID:Addgene_62817 Copy
Species: Escherichia coli
Genetic Insert: KanR
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:4361; Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23656183
Proper citation: RRID:Addgene_55750 Copy
Species: Xanthamonas
Genetic Insert: NS3
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Comments: Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_50611 Copy
Species: Xanthamonas
Genetic Insert: NS9
Vector Backbone Description: Backbone Marker:n/a; Backbone Size:3007; Vector Backbone:pTC14; Vector Types:Unspecified; Bacterial Resistance:Tetracycline
Comments: Note: Restriction digests show that this pNS series of plasmids is actually about 2.5kb, not 3.1kb as the sequence-based map below suggests. All important features are present in the plasmid and the construct should function as reported despite this size discrepancy.
Proper citation: RRID:Addgene_50617 Copy
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7535; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928
Proper citation: RRID:Addgene_48100 Copy
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7535; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928
Proper citation: RRID:Addgene_48097 Copy
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7535; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928
Proper citation: RRID:Addgene_48098 Copy
Genetic Insert: E. coli lacZ
Vector Backbone Description: Backbone Marker:Andreas Kaczmarczyk; Backbone Size:7300; Vector Backbone:pQF; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:23995928
Proper citation: RRID:Addgene_48094 Copy
Species: M. barkeri
Genetic Insert: tRNA synthetase
Vector Backbone Description: Backbone Size:6290; Vector Backbone:pDule; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline
Defining Citation: PMID:21678540
Proper citation: RRID:Addgene_49086 Copy
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