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Species: E. coli
Genetic Insert: E. coli RtcB
Vector Backbone Description: Backbone Marker:EMD / Novagen; Backbone Size:5230; Vector Backbone:pET-53-DEST; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:26001965
Comments: Use expression strain: BL21 (DE3) RIPL
Proper citation: RRID:Addgene_51282 Copy
Species: E. coli
Genetic Insert: FIS
Vector Backbone Description: Vector Backbone:pBAD24; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23893733
Proper citation: RRID:Addgene_51557 Copy
Species: E. coli
Genetic Insert: FIS
Vector Backbone Description: Backbone Marker:NEB; Vector Backbone:pTXB1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51564 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_52999 Copy
Species: E. coli
Genetic Insert: RhyB sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_53001 Copy
Species: E. coli
Genetic Insert: OxyS sRNA
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:21189298
Proper citation: RRID:Addgene_53006 Copy
Species: E. coli
Genetic Insert: mCherry
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin
Proper citation: RRID:Addgene_53032 Copy
Species: E. coli
Genetic Insert: fimE
Vector Backbone Description: Backbone Marker:Lim lab; Vector Backbone:unknown; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_53015 Copy
Species: E. coli
Genetic Insert: Streptavidin-Glutamate_Tag
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056174
Proper citation: RRID:Addgene_46367 Copy
Species: E. coli
Genetic Insert: ltDHFR(DD)
Vector Backbone Description: Backbone Marker:Garry Nolan ; Backbone Size:6943; Vector Backbone:pBMN; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:23991108
Comments: This N-terminal ltDHFR(DD) plasmid is the clone#22 in Table 1
Proper citation: RRID:Addgene_47076 Copy
Species: E. coli
Genetic Insert: etpBA
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4100; Vector Backbone:pBAD/myc-His A; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19707189
Comments: Please note that there is a minor mutation in etpB - E64G. This mutation should not affect function.
Proper citation: RRID:Addgene_53532 Copy
Species: E. coli
Genetic Insert: etpC
Vector Backbone Description: Backbone Size:3719; Vector Backbone:pACYC184; Vector Types:Bacterial Expression, pACYC184; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:19707189
Comments: Please note that there is a L to M mutation in etpC. This mutation should not affect function.
Proper citation: RRID:Addgene_53533 Copy
Species: e. coli
Genetic Insert: pLux
Vector Backbone Description: Backbone Size:1000; Vector Backbone:pLuxR; Vector Types:Unspecified; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24104480
Proper citation: RRID:Addgene_53544 Copy
Species: E. coli
Genetic Insert: αNTD
Vector Backbone Description: Vector Backbone:pBR322; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9121589
Comments: This plasmid is used in conjuction with the other Ann Hochschild Bacterial 2-hybrid system plasmids (Addgene plasmids 53730, 53731, 53732, 53733, and 53734) and must be used in the reporter strain, FW102 OL2–62 (Addgene item 53735). Please see the associated article for links to all of these plasmids:
http://www.addgene.org/browse/article/8393/
The following articles can provide more information on using the bacterial 2-hybrid system:
Dove, et al. 1997 PMID 9121589
Dove and Hochschild 1998 PMID 9499408
Deaconescu, et al. 2006 PMID 16469698
Deighan, et al. 2008 PMID 18832144
Kuznedelov, et al. 2002 PMID 11823642
Proper citation: RRID:Addgene_53731 Copy
Species: E. coli
Genetic Insert: BirA
Vector Backbone Description: Backbone Marker:Yves Durocher; Backbone Size:6000; Vector Backbone:pTT3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18296487
Proper citation: RRID:Addgene_64395 Copy
Species: E. Coli
Genetic Insert: Core Traptavidin-E6
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5400; Vector Backbone:pET21a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25111182
Proper citation: RRID:Addgene_59549 Copy
Species: e. coli
Genetic Insert: FLEX-mNaChBac-T2A-tdTomato
Vector Backbone Description: Backbone Size:5639; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25043046
Proper citation: RRID:Addgene_60658 Copy
Species: e. coli
Genetic Insert: mNaChBacMut-T2A-tdTomato
Vector Backbone Description: Backbone Size:5508; Vector Backbone:pCAG; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25043046
Proper citation: RRID:Addgene_60657 Copy
Species: e. coli
Genetic Insert: FLEX-mNaChBacMut-T2A-tdTomato
Vector Backbone Description: Backbone Size:5639; Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25043046
Proper citation: RRID:Addgene_60660 Copy
Species: E. Coli
Genetic Insert: LacI
Vector Backbone Description: Backbone Marker:Expressys; Backbone Size:4203; Vector Backbone:pZS5; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25035932
Proper citation: RRID:Addgene_60745 Copy
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