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Species: SARS-CoV-2
Genetic Insert: nsp10
Vector Backbone Description: Vector Backbone:pCOLADuet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198326
Comments: Unlike SARS-CoV-2 nsp10 that gets expressed in the context of a larger polypeptide, this SARS-CoV-2 nsp10 gets expressed from a 5' ATG, and thus might contain a methionine at the N-terminus that is not present in the nsp5-cleaved nsp10 in cells.
Proper citation: RRID:Addgene_169158 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-nsp5CS[VATLQ]-nsp14
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198326
Comments: We have included the predicted cleavage site for nsp5 found between nsp13 and nsp14 (VATLQ) between the 3xFlag tag and nsp14 in this construct, which should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
Proper citation: RRID:Addgene_169159 Copy
Species: SARS-CoV-2
Genetic Insert: 3xFlag-His-nsp5CS-nsp9
Vector Backbone Description: Vector Backbone:pLIB (biGBac); Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34198327
Comments: Baculovirus generation using Tn7 transposition (Bac-to-Bac).
Proper citation: RRID:Addgene_169177 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Nsp3 Mac1
Vector Backbone Description: Backbone Size:5358; Vector Backbone:pET22b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33853786
Proper citation: RRID:Addgene_169209 Copy
Species: SARS-CoV-2
Genetic Insert: 14His-SUMO-nsp7
Vector Backbone Description: Vector Backbone:pK27Sumo; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:34198323
Proper citation: RRID:Addgene_169186 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170190 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170191 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170194 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170202 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 RBD
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33248226
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170203 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 S (spike) B.1.429
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170200 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 S (spike) D614G
Vector Backbone Description: Vector Backbone:pDest-303; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32504802
Comments: DNA sequence optimized for mammalian expression
Proper citation: RRID:Addgene_170201 Copy
Species: SARS-CoV-2
Genetic Insert: Nucleocapsid
Vector Backbone Description: Vector Backbone:pHAGE; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.09.10.459410v2 for bioRxiv preprint.
Proper citation: RRID:Addgene_170466 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike
Vector Backbone Description: Backbone Size:4971; Vector Backbone:pCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33969320
Proper citation: RRID:Addgene_170389 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 NSP3 K977Q
Vector Backbone Description: Vector Backbone:pDON207; Vector Types:Mammalian Expression; Bacterial Resistance:Gentamicin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180057 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike T1027I
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180058 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike A570D
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180064 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike K417N
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180060 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 Spike L241-L242-A243-
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180070 Copy
Species: SARS-CoV-2
Genetic Insert: SARS-CoV-2 N S235F
Vector Backbone Description: Vector Backbone:pDON223; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:34709727
Proper citation: RRID:Addgene_180071 Copy
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