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Species: Homo sapiens
Genetic Insert: PTCH1 (hedgehog dependent promoter)
Vector Backbone Description: Backbone Size:2775; Vector Backbone:pMuLE ENTR MCS R4-R3; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31860685
Comments: Winklmayr M, Schmid C, Laner-Plamberger S, Kaser A, Aberger F, Eichberger T, et al. Non-consensus GLI binding sites in Hedgehog target gene regulation. BMC molecular biology. 2010;11(1):2
Proper citation: RRID:Addgene_113864 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of constitutively-inactive ARF6. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11386 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of constitutively-active ARF1. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11385 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of ARF6. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11382 Copy
Species: Danio rerio
Genetic Insert: he1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2640; Vector Backbone:pDONR P2R-P3; Vector Types:Zebrafish Danio expression; Bacterial Resistance:Kanamycin
Comments: Cloned from the construct provided by Jeff Mumm's Lab, pTol2 5xUAS:mRFP-NTRmut;HE-mCherry
Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93
Proper citation: RRID:Addgene_113881 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac2 with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11393 Copy
Species: Homo sapiens
Genetic Insert: cell division cycle 42
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Cdc42 with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11392 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac1(N17) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11395 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac1 with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11391 Copy
Species: Danio rerio
Genetic Insert: he1a
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2618; Vector Backbone:pDONR P2R-P3; Vector Types:Zebrafish Danio expression; Bacterial Resistance:Kanamycin
Comments: Cloned from the construct provided by Jeff Mumm's Lab, pTol2 5xUAS:YFP-p2A-NTRmutgi:HE:eYFP
Reference for the he1a promoter was first described in:
Xie et al., "Silencer-delimited transgenesis: NRSE/RE1 sequences promote neural-specific transgene expression
in a NRSF/REST-dependent manner."
BMC Biology 2012 10:93
https://doi.org/10.1186/1741-7007-10-93
Proper citation: RRID:Addgene_113879 Copy
Genetic Insert: CBF (notch dependent promoter)
Vector Backbone Description: Backbone Size:2665; Vector Backbone:pMuLE ENTR MCS L3-L2; Vector Types:Mammalian Expression, Gateway Cloning; Bacterial Resistance:Kanamycin
Defining Citation: PMID:31860685
Proper citation: RRID:Addgene_113868 Copy
Species: Homo sapiens
Genetic Insert: Adenylate Cyclase 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pAmCyan1-c1; Vector Types:Mammalian Expression, Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29903911
Comments: Please acknowledge the Principal Investigator, cite the article in which the plasmids were described.
Proper citation: RRID:Addgene_113905 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a YFP fusion to the C-terminus of ARF6. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP variant.
Proper citation: RRID:Addgene_11389 Copy
Species: Homo sapiens
Genetic Insert: golgi associated, gamma adaptin ear containing, ARF binding protein 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a YFP fusion to the N-terminus of the mutated GGA1 NGAT domain. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP variant.
Proper citation: RRID:Addgene_11388 Copy
Species: Homo sapiens
Genetic Insert: ADP Ribosylation Factor 6
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:16669702
Comments: Encodes a CFP fusion to the C-terminus of constitutively-active ARF6. CFP is the monomeric (A207K) version of ECFP.
Proper citation: RRID:Addgene_11387 Copy
Species: Homo sapiens
Genetic Insert: cell division cycle 42
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Cdc42(N17) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11400 Copy
Species: Homo sapiens
Genetic Insert: ras-related C3 botulinum toxin substrate 1
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15169870
Comments: Expresses Rac1(H40, 61L) with an N-terminal YFP marker. YFP is the monomeric (A207K) version of Citrine, a pH-insensitive YFP.
Proper citation: RRID:Addgene_11403 Copy
Species: Synthetic
Genetic Insert: gRNA
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:; Bacterial Resistance:Kanamycin
Defining Citation: PMID:29765036
Proper citation: RRID:Addgene_114005 Copy
Species: Synthetic
Genetic Insert: human codon optimized AsCas12a with C-terminal NLS and His-tag
Vector Backbone Description: Backbone Marker:Schier Lab (Addgene plasmid # 47327); Vector Backbone:pET-28b-Cas9-His; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114069 Copy
Species: Synthetic
Genetic Insert: entry vector for LbCas12a crRNAs
Vector Backbone Description: Backbone Marker:Joung Lab (Addgene plasmid # 42230); Vector Backbone:DR247; Vector Types:in vitro transcription; Bacterial Resistance:Kanamycin
Defining Citation: PMID:30742127
Proper citation: RRID:Addgene_114068 Copy
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