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Species: Homo sapiens
Genetic Insert: ubc13
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5800; Vector Backbone:PETSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22367539
Proper citation: RRID:Addgene_51131 Copy
Species: Homo sapiens
Genetic Insert: Cin85
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:6600; Vector Backbone:pCMV6-AN-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments:
Proper citation: RRID:Addgene_51137 Copy
Species: Homo sapiens
Genetic Insert: optimized sgRNA
Vector Backbone Description: Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24360272
Comments: gRNA target sequence GTGGCGTGACCTGTGGATGCTG
Proper citation: RRID:Addgene_51025 Copy
Species: Homo sapiens
Genetic Insert: Optimized sgRNA
Vector Backbone Description: Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24360272
Comments: gRNA target sequence GTTAGGGTTAGGGTTAGGGTTA
Proper citation: RRID:Addgene_51024 Copy
Species: Homo sapiens
Genetic Insert: MID2
Vector Backbone Description: Backbone Marker:Cox lab; Vector Backbone:pCMV-N2-6Myc; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11806752
Comments: Please note: hMID2 begins at residue 19 of the NCBI reference sequence.
Proper citation: RRID:Addgene_51035 Copy
Species: Homo sapiens
Genetic Insert: RALB
Vector Backbone Description: Vector Backbone:pLA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056301
Proper citation: RRID:Addgene_50989 Copy
Species: Homo sapiens
Genetic Insert: RALB
Vector Backbone Description: Vector Backbone:pLA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056301
Proper citation: RRID:Addgene_50983 Copy
Species: Homo sapiens
Genetic Insert: RALB
Vector Backbone Description: Vector Backbone:pLA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056301
Proper citation: RRID:Addgene_50982 Copy
Species: Homo sapiens
Genetic Insert: sgRNA library (enriched for ribosomal targets)
Vector Backbone Description: Backbone Marker:based on pLX304 (Root lab); Backbone Size:7500; Vector Backbone:pLX-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336569
Comments: The library is supplied as pooled DNA. You will receive 1 microgram of DNA in a volume of ~20 microliters.
IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
The backbone of this library is pLX-sgRNA (http://www.addgene.org/50662). See map below.
Proper citation: RRID:Addgene_51045 Copy
Species: Homo sapiens
Genetic Insert: sgRNA library (enriched for kinase targets)
Vector Backbone Description: Backbone Marker:based on pLX304 (Root lab); Backbone Size:7500; Vector Backbone:pLX-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336569
Comments: The library is supplied as pooled DNA. You will receive 1 microgram of DNA in a volume of ~20 microliters.
IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
The backbone of this library is pLX-sgRNA (http://www.addgene.org/50662). See map below.
Proper citation: RRID:Addgene_51044 Copy
Species: Homo sapiens
Genetic Insert: sgRNA library (enriched for nuclear targets)
Vector Backbone Description: Backbone Marker:based on pLX304 (Root lab); Backbone Size:7500; Vector Backbone:pLX-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336569
Comments: The library is supplied as pooled DNA. You will receive 1 microgram of DNA in a volume of ~20 microliters.
IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
The backbone of this library is pLX-sgRNA (http://www.addgene.org/50662). See map below.
Proper citation: RRID:Addgene_51047 Copy
Species: Homo sapiens
Genetic Insert: sgRNA library (enriched for genes of unknown function)
Vector Backbone Description: Backbone Marker:based on pLX304 (Root lab); Backbone Size:7500; Vector Backbone:pLX-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336569
Comments: The library is supplied as pooled DNA. You will receive 1 microgram of DNA in a volume of ~20 microliters.
IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
The backbone of this library is pLX-sgRNA (http://www.addgene.org/50662). See map below.
Proper citation: RRID:Addgene_51043 Copy
Species: Homo sapiens
Genetic Insert: ARAF
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABEpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24569458
Proper citation: RRID:Addgene_51078 Copy
Species: Homo sapiens
Genetic Insert: H2B-EYFP
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4733; Vector Backbone:pEYFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
Proper citation: RRID:Addgene_51002 Copy
Species: Homo sapiens
Genetic Insert: CRAF
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABEpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51126 Copy
Species: Homo sapiens
Genetic Insert: H2B-EGFP
Vector Backbone Description: Backbone Size:8344; Vector Backbone:pLenti.Syn(0.5).eGFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2.
Proper citation: RRID:Addgene_51004 Copy
Species: Homo sapiens
Genetic Insert: CRAF
Vector Backbone Description: Backbone Size:5200; Vector Backbone:pBABEpuro; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51127 Copy
Species: Homo sapiens
Genetic Insert: homeobox A9
Vector Backbone Description: Backbone Marker:Dr. Richard Mulligan; Backbone Size:12152; Vector Backbone:pINDUCER21 (ORF-EG); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24094326
Comments: NOTE: The map and depositor sequence are of the empty backbone ONLY. Addgene does not have sequence for the fully assembled plasmid. Sequence for much of the insert can be found in Addgene's sequencing results.
Proper citation: RRID:Addgene_51302 Copy
Species: Homo sapiens
Genetic Insert: v-ets avian erythroblastosis virus E26 oncogene homolog
Vector Backbone Description: Backbone Marker:Dr. Richard Mulligan; Backbone Size:12152; Vector Backbone:pINDUCER21 (ORF-EG); Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24094326
Comments: NOTE: The map and depositor sequence are of the empty backbone ONLY. Addgene does not have sequence for the fully assembled plasmid. Sequence for much of the insert can be found in Addgene's sequencing results.
Proper citation: RRID:Addgene_51301 Copy
Species: Homo sapiens
Genetic Insert: cofilin 1
Vector Backbone Description: Backbone Marker:R.Y. Tsien, PNAS 2002 v99 pp7877–7882; Vector Backbone:pmRFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24391794
Comments: This cofilin plasmid contains some silent mutations to make it resistant to an siRNA used in the Bamburg lab. The mutations change the wildtype sequence (AAG TCT TCA ACG CCA GAG GAG) into AAG AGC TCA ACC CCA GAG GAG.
Proper citation: RRID:Addgene_51279 Copy
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