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Species: Rattus norvegicus
Genetic Insert: mRuby2-P2A-GCaMP6m
Vector Backbone Description: Vector Backbone:pAAV; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27284193
Comments: The discrepancies between the full sequence and Addgene's QC sequence should not have any functional consequence.
Proper citation: RRID:Addgene_51473 Copy
Species: Rattus norvegicus
Genetic Insert: Chimeric Synaptophysin-mCherry-Luciferase
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24529383
Proper citation: RRID:Addgene_51819 Copy
Species: Rattus norvegicus
Genetic Insert: MIBP1
Vector Backbone Description: Backbone Size:4800; Vector Backbone:pCAGGS; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22294689
Proper citation: RRID:Addgene_51870 Copy
Species: Rattus norvegicus
Genetic Insert: MIBP1
Vector Backbone Description: Backbone Marker:Promega; Backbone Size:4000; Vector Backbone:pCI; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11872163
Comments: Makino, R., Akiyama, K., Yasuda, J., Mashiyama, S., Honda, S., Sekiya, T., & Hayashi, K. (1994). Cloning and characterization of a c-myc intron binding protein (MIBP1). Nucleic acids research, 22(25), 5679-5685.
Proper citation: RRID:Addgene_51872 Copy
Species: Rattus norvegicus
Genetic Insert: CLC3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:11997263
Comments: Insert represents a portion of the 5’UTR and the full open reading frame of ClC-3 beginning at nucleotide 251 according to GenBank Accession number D17521.
Proper citation: RRID:Addgene_52423 Copy
Species: Rattus norvegicus
Genetic Insert: CLC3
Vector Backbone Description: Backbone Marker:Clontech; Vector Backbone:pEGFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:15057754
Comments: Insert represents a portion of the 5’UTR and the full open reading frame of ClC-3 beginning at nucleotide 251 according to GenBank Accession number D17521.
Proper citation: RRID:Addgene_52424 Copy
Species: Rattus norvegicus
Genetic Insert: GLUT4
Vector Backbone Description: Backbone Marker:Bogan lab, unpublished; Backbone Size:5783; Vector Backbone:pB; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11416153
Comments: Plasmid is also called pB-G7. We use FACS to select infected cells that express GFP. There is a short linker between GLUT4 and GFP.
Proper citation: RRID:Addgene_52872 Copy
Species: Rattus norvegicus
Genetic Insert: Kir4.1
Vector Backbone Description: Vector Backbone:pZac 2.1; Vector Types:Mammalian Expression, AAV, Adeno-Associated Virus (); Bacterial Resistance:Ampicillin
Defining Citation: PMID:24686787
Proper citation: RRID:Addgene_52874 Copy
Species: Rattus norvegicus
Genetic Insert: PSD-95
Vector Backbone Description: Backbone Marker:Evrogen; Backbone Size:4733; Vector Backbone:pTagRFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: HindII and EcoRI were used to cut PSD-95 from PSD-95-pGW1 (received from David Bredt) and ligate it into pTagRFP-N. PSD-95 tyrosine 12 was mutated to glutamate (Y12E) by extending a complementary set of primers (PSD95Y12E-forward: 5’-GAC AAC CAA GAA AGA GCG CTA CCA AGA TGA AGA CAC G-3’, PSD95Y12E-reverse: 5'-CGT GTC TTC ATC TTG GTA GCG CTC TTT CTT GGT TGT C-3') along the whole template plasmid using Q5 high-fidelity DNA polymerase (NEB). DpnI was subsequently used to digest non-mutated, methylated template DNA prior to transformation.
Proper citation: RRID:Addgene_52672 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN4
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 493–511 from the start sequence of rat α-actinin-4 (the sense chain was 5’-gactatccaggagatgcagttcaagagactgcatctcctggatagtctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52679 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN1
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 476–494 from the start sequence of rat α-actinin-1 (the sense chain was 5’-gatctccaacgtcaacaagttcaagagacttgttgacgttggagatctttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52676 Copy
Species: Rattus norvegicus
Genetic Insert: ACTN2
Vector Backbone Description: Backbone Marker:Hell lab, Addgene plasmid #52675; Vector Backbone:pSIL-EGFP; Vector Types:RNAi; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19028690
Comments: EGFP is a transfection marker, not a fusion.
RNAi targeted at 224–242 from the start sequence of rat α-actinin-2 (the sense chain was 5’-tggcctgatggatcatgagttcaagagactcatgatccatcaggccatttttt-3’. The 19-nucleotide sense sequence and the inverted antisense sequence were connected by a 9-nucleotide spacer to allow stem-loop formation. At the 3’ end, a penta-thymidine motif provided a polymerase III termination site. siRNAs were cloned into pSilencer™ vector for expression of the respective sequences under the U6 promotor.
Proper citation: RRID:Addgene_52677 Copy
Species: Rattus norvegicus
Genetic Insert: GAL4(1-147)-CREB-S133A
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4767; Vector Backbone:pcDNAI-AMP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7958915
Proper citation: RRID:Addgene_46770 Copy
Species: Rattus norvegicus
Genetic Insert: GAL4(1-147)-CREB
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:4767; Vector Backbone:pcDNAI-AMP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:7958915
Proper citation: RRID:Addgene_46769 Copy
Species: Rattus norvegicus
Genetic Insert: soluble guanylate cyclase 1alpha3
Vector Backbone Description: Backbone Marker:Homemade; Backbone Size:6642; Vector Backbone:pMA3211; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22637478
Comments: Please note that though there are some differences between Addgene's quality control sequence and the depositor's assembled sequence, this plasmid should function as reported.
Proper citation: RRID:Addgene_46874 Copy
Species: Rattus norvegicus
Genetic Insert: soluble guanylate cyclase1 beta 3
Vector Backbone Description: Backbone Size:5684; Vector Backbone:pMA1662; Vector Types:Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22637478
Proper citation: RRID:Addgene_46877 Copy
Species: Rattus norvegicus
Genetic Insert: MAP4K3
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5446; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:11384986
Proper citation: RRID:Addgene_47402 Copy
Species: Rattus norvegicus
Genetic Insert: SAPAP1
Vector Backbone Description: Vector Backbone:pCMV5; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:9115257
Proper citation: RRID:Addgene_47940 Copy
Species: Rattus norvegicus
Genetic Insert: Syntaxin 1A
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53236 Copy
Species: Rattus norvegicus
Genetic Insert: VAMP2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:23536066
Proper citation: RRID:Addgene_53233 Copy
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