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Species: Synthetic
Genetic Insert: oChIEF(E163A/T199C)
Vector Backbone Description: Backbone Marker:custom; Backbone Size:5100; Vector Backbone:pAAV-CaMKIIa-WPRE-HGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The addition of the E163A and T199C mutations in oChIEF impart higher photocurrent amplitude while retaining fast kinetic properties. This variant is proposed as an alternative to ChETA variants that exhibit smaller photocurrents and more toxicity.
Proper citation: RRID:Addgene_51092 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:8089; Vector Backbone:pSIR; Vector Types:Mammalian Expression, Retroviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:25051498
Proper citation: RRID:Addgene_51135 Copy
Species: Synthetic
Genetic Insert: ChIEF(E162A/T198C)
Vector Backbone Description: Backbone Marker:custom; Backbone Size:1047; Vector Backbone:pAAV-EF1a-DIO-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: The addition of the E162A and T198C mutations in ChIEF impart higher photocurrent amplitude while retaining fast kinetic properties. This variant is proposed as an alternative to ChETA variants that exhibit smaller photocurrents and more toxicity.
Proper citation: RRID:Addgene_51095 Copy
Species: Homo sapiens
Genetic Insert: ubc13
Vector Backbone Description: Backbone Marker:Novagen; Backbone Size:5800; Vector Backbone:PETSUMO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22367539
Proper citation: RRID:Addgene_51131 Copy
Species: Homo sapiens
Genetic Insert: Cin85
Vector Backbone Description: Backbone Marker:Origene; Backbone Size:6600; Vector Backbone:pCMV6-AN-GFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments:
Proper citation: RRID:Addgene_51137 Copy
Species: Synthetic
Genetic Insert: NOSp-NPTII-OCST
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH47732; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51144 Copy
Species: Homo sapiens
Genetic Insert: optimized sgRNA
Vector Backbone Description: Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24360272
Comments: gRNA target sequence GTGGCGTGACCTGTGGATGCTG
Proper citation: RRID:Addgene_51025 Copy
Species: Homo sapiens
Genetic Insert: Optimized sgRNA
Vector Backbone Description: Vector Backbone:pSico; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24360272
Comments: gRNA target sequence GTTAGGGTTAGGGTTAGGGTTA
Proper citation: RRID:Addgene_51024 Copy
Species: Synthetic
Genetic Insert: NOSp-BAR-NOST
Vector Backbone Description: Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH47732; Vector Types:; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_51145 Copy
Genetic Insert: oChD-citrine
Vector Backbone Description: Backbone Size:4599; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19254539
Comments: WPRE is inserted after the citrine before SV40 polyA.
Proper citation: RRID:Addgene_50976 Copy
Species: Synthetic
Genetic Insert: ChEF-citrine
Vector Backbone Description: Backbone Size:4599; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19254539
Comments: WPRE is inserted after the citrine before SV40 polyA.
Proper citation: RRID:Addgene_50975 Copy
Species: Synthetic
Genetic Insert: ChIEF-tdTomato
Vector Backbone Description: Backbone Size:4599; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19254539
Proper citation: RRID:Addgene_50977 Copy
Species: Escherichia coli
Genetic Insert: AcrR
Vector Backbone Description: Vector Backbone:pET21a; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24316737
Comments: UCSF Case No. SF11-016-2; U.S. Patent Application 13/489,205. GeneArt inserted the synthesized fragment into a pET21a vector.
Proper citation: RRID:Addgene_51148 Copy
Species: Homo sapiens
Genetic Insert: RALB
Vector Backbone Description: Vector Backbone:pLA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056301
Proper citation: RRID:Addgene_50989 Copy
Species: Homo sapiens
Genetic Insert: RALB
Vector Backbone Description: Vector Backbone:pLA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056301
Proper citation: RRID:Addgene_50983 Copy
Species: Homo sapiens
Genetic Insert: RALB
Vector Backbone Description: Vector Backbone:pLA; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24056301
Proper citation: RRID:Addgene_50982 Copy
Species: Homo sapiens
Genetic Insert: sgRNA library (enriched for ribosomal targets)
Vector Backbone Description: Backbone Marker:based on pLX304 (Root lab); Backbone Size:7500; Vector Backbone:pLX-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336569
Comments: The library is supplied as pooled DNA. You will receive 1 microgram of DNA in a volume of ~20 microliters.
IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
The backbone of this library is pLX-sgRNA (http://www.addgene.org/50662). See map below.
Proper citation: RRID:Addgene_51045 Copy
Species: Homo sapiens
Genetic Insert: sgRNA library (enriched for kinase targets)
Vector Backbone Description: Backbone Marker:based on pLX304 (Root lab); Backbone Size:7500; Vector Backbone:pLX-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336569
Comments: The library is supplied as pooled DNA. You will receive 1 microgram of DNA in a volume of ~20 microliters.
IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
The backbone of this library is pLX-sgRNA (http://www.addgene.org/50662). See map below.
Proper citation: RRID:Addgene_51044 Copy
Species: Homo sapiens
Genetic Insert: sgRNA library (enriched for nuclear targets)
Vector Backbone Description: Backbone Marker:based on pLX304 (Root lab); Backbone Size:7500; Vector Backbone:pLX-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336569
Comments: The library is supplied as pooled DNA. You will receive 1 microgram of DNA in a volume of ~20 microliters.
IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
The backbone of this library is pLX-sgRNA (http://www.addgene.org/50662). See map below.
Proper citation: RRID:Addgene_51047 Copy
Species: Homo sapiens
Genetic Insert: sgRNA library (enriched for genes of unknown function)
Vector Backbone Description: Backbone Marker:based on pLX304 (Root lab); Backbone Size:7500; Vector Backbone:pLX-sgRNA; Vector Types:Mammalian Expression, Lentiviral, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:24336569
Comments: The library is supplied as pooled DNA. You will receive 1 microgram of DNA in a volume of ~20 microliters.
IMPORTANT NOTE: Though each sub-pool is enriched for its respective target sgRNAs, they are not exclusive and elements of the entire library may be present in each sub-pool.
The backbone of this library is pLX-sgRNA (http://www.addgene.org/50662). See map below.
Proper citation: RRID:Addgene_51043 Copy
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