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Species: Other
Genetic Insert: Rep2/Cap2
Vector Backbone Description: Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.
Proper citation: RRID:Addgene_104963 Copy
Species: Other
Genetic Insert: Rep2/Cap5
Vector Backbone Description: Vector Backbone:pUC19 with additional elements; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin
Comments: Contains the p5 promoter upstream of RepCap as well as the p5 promoter downstream of RepCap in the 3' orientation. This configuration of promoters has been shown to improve viral production yields.
Proper citation: RRID:Addgene_104964 Copy
Species: Other
Genetic Insert: Mus musculus Akap1(1-31) M16L: PTRSANS[GA]4ILSRM:TagBFP2: FKBP1A(3-108): [GGSA]4GG:L. monocytogenes PI-PLC(1-187)
Vector Backbone Description: Vector Backbone:pTagBFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32211893
Proper citation: RRID:Addgene_139309 Copy
Species: Other
Genetic Insert: SNIPR_GFP
Vector Backbone Description: Backbone Size:3224; Vector Backbone:pCOLAduet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32109416
Comments: SNIPR regulating GFPmut3b with an ASV degradation tag
Proper citation: RRID:Addgene_139463 Copy
Species: Other
Genetic Insert: SNIPR_lacZ
Vector Backbone Description: Backbone Size:3324; Vector Backbone:pCOLAduet; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32109416
Comments: SNIPR regulating a lacZ reporter protein
Proper citation: RRID:Addgene_139464 Copy
Species: Other
Genetic Insert: Target_mutGU33
Vector Backbone Description: Backbone Size:5527; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32109416
Comments: Non-cognate target for the SNIPR with a mismatch at base 33 leading to a G-U base pair with SNIPR
Proper citation: RRID:Addgene_139468 Copy
Species: Other
Genetic Insert: 5BoxB
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19383768
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146091 Copy
Species: Other
Genetic Insert: Rluc
Vector Backbone Description: Vector Backbone:pCI-neo; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:19383768
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146090 Copy
Species: Other
Genetic Insert: GFP52
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146099 Copy
Species: Other
Genetic Insert: GFP-PTC
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:18974281
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_146154 Copy
Species: Other
Genetic Insert: 6xbsMS2-random
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:27009120
Comments: Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148431 Copy
Species: Other
Genetic Insert: K0-Rluc
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: reporter for Ribosome Quality control Plasmids from this collection were made available to the research community and donated after the lab closed. Please note that the sequence provided by the depositing laboratory may be theoretical/predicted or based on Sanger sequencing results. There may be discrepancies between sequencing results obtained by Addgene and the depositor sequence. If an Addgene verified sequence is not available, please contact [email protected] before placing your order to request that our lab fully sequence the plasmid.
Proper citation: RRID:Addgene_148846 Copy
Species: Other
Genetic Insert: SARS-CoV-2 nsp6
Vector Backbone Description: Backbone Size:6525; Vector Backbone:pTwist_CMV_Hygro_6; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: NCBI reference sequence: YP_009725302.1
Proper citation: RRID:Addgene_152030 Copy
Species: Other
Genetic Insert: ICP0
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting mCherry for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22976303
Proper citation: RRID:Addgene_134560 Copy
Species: Other
Genetic Insert: ICP0
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting mCherry for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:22976303
Comments: Cherry-ICP0FxE-C3 contains a V886A difference compared to the reference sequence. The functional outcome is unknown but this enzyme is catalytically inert as designed.
Proper citation: RRID:Addgene_134562 Copy
Species: Other
Genetic Insert: YFP-ICP0
Vector Backbone Description: Vector Backbone:pLU; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22976303
Proper citation: RRID:Addgene_134563 Copy
Species: Other
Genetic Insert: YFP-ICP0
Vector Backbone Description: Vector Backbone:pLU; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:22976303
Comments: pLU-YFP-ICP0FxE contains A514P and V886A differences compared to YP_009137074.1. The functional consequences of these are unknown but the protein is catalytically inert by design.
Proper citation: RRID:Addgene_134564 Copy
Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting mCherry for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134567 Copy
Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134568 Copy
Species: Other
Genetic Insert: tTA-ER
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4700; Vector Backbone:pEYFP-C3; vector was modified by substituting CFP for YFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:20422051
Proper citation: RRID:Addgene_134569 Copy
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