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  • Bacterial Resistance:ampicillin and gentamicin (facet)

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pTSN6
 
Resource Report
Resource Website 		RRID:Addgene_131318 Full-length tetO array (XbaI-NheI fragment of pLAU44) Synthetic Ampicillin and Gentamicin PMID:31821884 TetO arrays are unstable and dual selection with Ampicillin and Gentamycin are essential for the maintenance of the array. Confirm full-length plasmid by restriction digest and Sanger sequencing of any clones prior to use. Some minor sequence discrepancies located in the TetO repeat array exist between Sanger and NGS based data. These are not of functional concern for use of the plasmid. Backbone Size:6803; Vector Backbone:pMF272; Vector Types:Unspecified; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:05:16 0
pFL
 
Resource Report
Resource Website
1+ mentions 		RRID:Addgene_110739 Ampicillin and Gentamicin PMID:17117155 Backbone Size:5450; Vector Backbone:pFL; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:01:43 2
pBFC0625
 
Resource Report
Resource Website 		RRID:Addgene_186691 tnsA Vibrio cholerae Ampicillin and Gentamicin PMID:34873292 Clone Tn barcodes using the 2xBsmBI cloning site. Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:23:44 0
pBFC1207
 
Resource Report
Resource Website 		RRID:Addgene_186694 tnsA Vibrio cholerae Ampicillin and Gentamicin PMID:34873292 Clone guides using the 2xBsaI cloning site. Clone Tn barcodes using the 2xBsmBI cloning site. This vector is identical to pBFC0619, but it includes a dual restriction site (AsiSI+SbfI) flanking the Tn right end to reduce donor vector during ET-Seq. Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:23:44 0
pBFC0694
 
Resource Report
Resource Website 		RRID:Addgene_186693 tnsB Scytonema hofmanni Ampicillin and Gentamicin PMID:34873292 Clone Tn barcodes using the 2xBsmBI cloning site. Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:23:43 0
pBFC0619
 
Resource Report
Resource Website 		RRID:Addgene_186690 tnsA Vibrio cholerae Ampicillin and Gentamicin PMID:34873292 Clone guides using the 2xBsaI cloning site. Clone Tn barcodes using the 2xBsmBI cloning site. Vector Backbone:R6K conditional origin, bla, RP4 oriT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:23:47 0
pAbaMCi-GFP-BsaI_pJMP2748
 
Resource Report
Resource Website 		RRID:Addgene_208888 dCas9, sgRNA expression cassette, sfGFP Ampicillin and Gentamicin PMID:38126769 Vector Backbone:R6K; Vector Types:Bacterial Expression, pir dependent origin of replication; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:28:28 0
pFastBac1_F734Y-hDPP9_6His
 
Resource Report
Resource Website 		RRID:Addgene_254905 Dipeptidyl peptidase 9 Homo sapiens Ampicillin and Gentamicin PMID:39552560 Backbone Size:4683; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin Changed Phenylalanine 734 to Tyrosine. 2026-08-15 01:36:05 0
pFastBac1_F253Y/F734-hDPP9-6His
 
Resource Report
Resource Website 		RRID:Addgene_254906 Dipeptidyl peptidase 9 Homo sapiens Ampicillin and Gentamicin PMID:39552560 Backbone Size:4683; Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin Changed phenylalanine 253 to tyrosine and phenylalanine 734 to tyrosine 2026-08-15 01:36:05 0
pMB.002
 
Resource Report
Resource Website 		RRID:Addgene_228668 Ampicillin and Gentamicin PMID:39814169 Spacer Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin GentR R98K 2026-08-15 01:31:31 0
pMB.BIG1a
 
Resource Report
Resource Website 		RRID:Addgene_228675 Ampicillin and Gentamicin PMID:39814169 Spacer with PmeI cleavage site and biGBac linker sequence A&B Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin GentR R98K 2026-08-15 01:31:31 0
pMB.BIG1b
 
Resource Report
Resource Website 		RRID:Addgene_228676 Ampicillin and Gentamicin PMID:39814169 Spacer with PmeI cleavage site and biGBac linker sequence B&C Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin GentR R98K 2026-08-15 01:31:31 0
pMB.BIG1d
 
Resource Report
Resource Website 		RRID:Addgene_228678 Ampicillin and Gentamicin PMID:39814169 Spacer with PmeI cleavage site and biGBac linker sequence D&E Vector Backbone:pFastBac1; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin GentR R98K 2026-08-15 01:31:31 0
pTn7-SCOUT21.RY
 
Resource Report
Resource Website 		RRID:Addgene_201006 mcherry Synthetic Ampicillin and Gentamicin PMID:38715147 Ec100D pir+ genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell. This strain is naturally resistant to streptomycin Backbone Marker:Philip Poole lab; Backbone Size:5442; Vector Backbone:pTn7-SCOUT21; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:29:41 0
pTn7-SCOUT11.B
 
Resource Report
Resource Website 		RRID:Addgene_201005 TagBFP Synthetic Ampicillin and Gentamicin PMID:38715147 Ec100D pir+ genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell. This strain is naturally resistant to streptomycin Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:29:41 0
pFastBac dual_Human full length CDK7(W132R, S164D, T170E)
 
Resource Report
Resource Website 		RRID:Addgene_217013 CDK7(W132R, S164D, T170E) Homo sapiens Ampicillin and Gentamicin PMID:38870939 Vector Backbone:pFastBac dual; Vector Types:Insect Expression; Bacterial Resistance:Ampicillin and Gentamicin CDK7(W132R, S164D, T170E) 2026-08-15 01:29:53 0
pUC18T-mini-Tn7T-Gm-Pc-mNeonGreen
 
Resource Report
Resource Website 		RRID:Addgene_205022 mNeonGreen Synthetic Ampicillin and Gentamicin PMID:29449848 Backbone Marker:Herbert Schweizer; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin mNeonGreen is codon optimized for expression in P. fluorescens 2026-08-15 01:27:45 0
pTn7-SCOUT11.R
 
Resource Report
Resource Website 		RRID:Addgene_201003 mCherry Synthetic Ampicillin and Gentamicin PMID:38715147 Ec100D pir+ genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir+(DHRF) Maintains plasmids at ~15 copies per cell. This strain is naturally resistant to streptomycin The plasmid appears to be prone to multimerization. Multimerization often does not impact plasmid function, and the depositor has confirmed that the plasmid performs as expected in their hands, but may reduce transformation efficiencies. Backbone Marker:Philip Poole lab; Backbone Size:5139; Vector Backbone:pTn7-SCOUT11; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:30:46 0
pTn7-SCOUT11
 
Resource Report
Resource Website 		RRID:Addgene_200991 Ampicillin and Gentamicin PMID:38715147 Ec100D pir-116 genotype: F- mcrA Δ(mrr-hsdRMS-mcrBC) ϕ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ- rpsL nupG pir-116(DHRF) Maintains plasmids at ~250 copies per cell. This strain is naturally resistant to streptomycin The plasmid appears to be prone to multimerization. Multimerization often does not impact plasmid function, and the depositor has confirmed that the plasmid performs as expected in their hands, but may reduce transformation efficiencies. Backbone Marker:Philip Poole lab; Backbone Size:5135; Vector Backbone:pTn7-SCOUT10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:30:46 0
pUC18T-mini-Tn7T-Gm-CC
 
Resource Report
Resource Website 		RRID:Addgene_246943 Type IF CRISPR-Cas system from Pseudomonas aeruginosa PA14 Pseudomonas aeruginosa PA14 Ampicillin and Gentamicin PMID:40904105 Synthetic Type IF CRISPR-Cas system based on the sequence from Pseudomonas aeruginosa PA14 with a minimised version of the CRISPR 2 array containing a non-targeting spacer with BbsI restriction sites for cloning. Native promoter regions were cloned as the intergenic region between the CRISPR components and upstream genes. MluI, NsiI, NotI, NcoI, AscI restriction sites were added to make the system modular. Backbone Marker:Herbert Schweizer (Addgene #63121); Backbone Size:4500; Vector Backbone:pUC18T-mini-Tn7T-Gm; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Ampicillin and Gentamicin 2026-08-15 01:35:06 0

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