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  • Bacterial Resistance:bleocin (zeocin) (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pBudORF2-CH
 
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1+ mentions
RRID:Addgene_51289 L1 ORF2 Homo sapiens Bleocin (Zeocin) PMID:21572950 This plasmid was created using the codon optimized L1RP as a source for the ORF2 coding sequence. Backbone Marker:Invitrogen; Backbone Size:4595; Vector Backbone:pBudCE4.1; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:16:15 2
pCri-15b
 
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RRID:Addgene_61336 Green fluorescent protein Bleocin (Zeocin) PMID:25386923 Vector Backbone:pPICZA; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:17:41 0
pBoPyV2a-S22
 
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RRID:Addgene_62356 Full genome of BoPyV2a isolate S22 Viruses (Polyomaviridae) Bleocin (Zeocin) PMID:25568187 Genome can be liberated by digestion with SacII Backbone Marker:Christopher Buck lab, Addgene plasmid 24755; Backbone Size:2131; Vector Backbone:pFunnyfarm; Vector Types:Viral clone; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:17:52 0
pBoPyV3-S22
 
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RRID:Addgene_62368 Full genome of BoPyV3 isolate S22 Viruses (Polyomaviridae) Bleocin (Zeocin) PMID:25568187 Genome can be liberated by digestion with EcoRI Backbone Marker:Christopher Buck lab, Addgene plasmid 24755; Backbone Size:2131; Vector Backbone:pFunnyfarm; Vector Types:Viral clone; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:17:52 0
pBoPyV3-S23
 
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RRID:Addgene_62369 Full genome of BoPyV3 isolate S23 Viruses (Polyomaviridae) Bleocin (Zeocin) PMID:25568187 Genome can be liberated by digestion with EcoRI Backbone Marker:Christopher Buck lab, Addgene plasmid 24755; Backbone Size:2131; Vector Backbone:pFunnyfarm; Vector Types:Viral clone; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:17:52 0
pCoofy42
 
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RRID:Addgene_55190 Bleocin (Zeocin) PMID:23410102 C-terminal tags are separated by stop codons. Depending on the LP2 primer used in SLIC, the desired C-terminal tag(s) can be fused to the target gene. Use the one of the following linearization primers pairs for SLIC cloning. Choose one LP1 forward vector primer and one LP2 reverse vector primer: LP1 forward vector primer: SLIC Primer (3C site) 5' GGGCCCCTGGAACAGAACTTCCAG 3' LP2 - select an LP2 primer below depending on which C-terminal tags you want to include fused to your gene of interest: none SLIC Primer 5' CGCCATTAACCTGATGTTCTGGGG 3' 10His- SLIC Primer 5`GAGCATCATCATCATCACCAC 3' StrepOne - SLIC Primer 5`AGCGCTTGGAGCCACCCGCAG 3' S-Tag - SLIC Primer 5`AAAGAAACCGCTGCTGCTAAATTCG 3' HPC4 - SLIC Primer 5`GAGGACCAGGTGGACCCCCGG 3' Æ54CPD - SLIC Primer 5`GGCAGCGGCAAGATCCTGCAC 3' Test each preparation of the plasmid by transforming it into non-resistant cells (ex. DH5a) to ensure negative selection by ccdB kills all the transformed cells as expected. Backbone Marker:Life Technologies; Backbone Size:4871; Vector Backbone:pPICZalpha C; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:16:47 0
pIZ-Flag6His-BmAgo3
 
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RRID:Addgene_50559 BmAgo3 Bombyx mori Bleocin (Zeocin) PMID:19460866 Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pIZ/V5-His; Vector Types:Insect Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:16:07 0
p8RwB
 
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1+ mentions
RRID:Addgene_48733 dsRed-Express Discosoma sp Bleocin (Zeocin) PMID:17603495 This plasmid is nearly identical to pRwB (http://www.addgene.org/48734), except that it contains a 2kb stuffer region to bring its size closer to the ~8kb native papillomavirus genome. For more information on using this plasmid, please see the following website: http://home.ccr.cancer.gov/Lco/target.htm Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:15:53 2
pRwB
 
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1+ mentions
RRID:Addgene_48734 dsRed-Express Discosoma sp Bleocin (Zeocin) PMID:19073722 Note that this plasmid is substantially smaller than the ~8kb native papillomavirus genome. There is a second version of this plasmid with a 2kb stuffer region that is available here: http://www.addgene.org/48733 . Further information can be found here: http://home.ccr.cancer.gov/Lco/target.htm Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:15:53 1
M-tdTom-SP
 
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1+ mentions
RRID:Addgene_48677 TAL binding site w/ GTCCCCTCCACCCCACAGTG protospacer, SP-PAM, and tdTomato reporter. Compatible with S. pyogenes Cas9 Synthetic Bleocin (Zeocin) PMID:24076762 Vector Backbone:Unknown; Vector Types:CRISPR; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:15:53 2
C321.ΔA.exp
 
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10+ mentions
RRID:Addgene_49018 Recoded E. coli with all UAG codons and RF1 removed (UAG termination abolished); decreased mutation rate; 37C growth tolerated E. coli Bleocin (Zeocin) PMID:24136966 E. coli MG1655 Delta(ybhB-bioAB)::zeoR Delta prfA; all 321 UAG codons changed to UAA This strain is mutS+, which causes a normal spontaneous mutation frequency of ~1E-10. This strain is auxotrophic for d-biotin. The genome sequence of a similar strain (C321.deltaA) was deposited at NCBI (accession # CP006698.1). Vector Backbone:E. coli genome; Vector Types:Bacterial Expression; Bacterial Resistance:Bleocin (Zeocin) See genbank file 2026-08-15 01:15:55 31
pfwB
 
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1+ mentions
RRID:Addgene_37329 GFP Aequorea victoria Bleocin (Zeocin) PMID:15709003 Vector Backbone:custom; Vector Types:Mammalian Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:14:14 4
AtPIN1
 
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RRID:Addgene_117088 AtPIN1 Arabidopsis thaliana Bleocin (Zeocin) Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:50 0
AtPIN2
 
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RRID:Addgene_117089 AtPIN2 Arabidopsis thaliana Bleocin (Zeocin) Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:50 0
MtPT4-eGFP
 
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RRID:Addgene_117095 MtPT4-eGFP Other Bleocin (Zeocin) Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:50 0
MtPT4
 
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RRID:Addgene_117096 MtPT4 Other Bleocin (Zeocin) Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:50 0
AtNPY1
 
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RRID:Addgene_117090 AtNPY1 Arabidopsis thaliana Bleocin (Zeocin) Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:50 0
SLAC-deltaCdeltaN
 
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RRID:Addgene_117103 SLAC-deltaCdeltaN Arabidopsis thaliana Bleocin (Zeocin) Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:50 0
SLAC-deltaC
 
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RRID:Addgene_117102 SLAC-deltaC Arabidopsis thaliana Bleocin (Zeocin) Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:51 0
SLAH3
 
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RRID:Addgene_117105 SLAH3 Arabidopsis thaliana Bleocin (Zeocin) Vector Backbone:pPICZc; Vector Types:Yeast Expression; Bacterial Resistance:Bleocin (Zeocin) 2026-08-15 01:02:51 0

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