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Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:Gateway Cloning; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51778 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtMIR390a-B/c
Vector Backbone Description: Backbone Size:8754; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51775 Copy
Species: Arabidopsis thaliana
Genetic Insert: AtTAS1c-B/c
Vector Backbone Description: Backbone Size:2580; Vector Backbone:pENTR; Vector Types:GATEWAY-compatible entry clone; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24647477
Proper citation: RRID:Addgene_51774 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54382 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54381 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54388 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54386 Copy
Vector Backbone Description: Backbone Marker:Icon Genetics; Vector Backbone:Xpre-K (pICH50505) ; Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54384 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54393 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54392 Copy
Vector Backbone Description: Backbone Marker:CAMBIA; Vector Backbone:Xpre2-K (pCAMBIA); Vector Types:plant expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:24551083
Comments: binary expression vector. LIII/LV backbone.
Proper citation: RRID:Addgene_54391 Copy
Species: Pseudomonas putida strain CBB5
Genetic Insert: ndmA
Vector Backbone Description: Backbone Marker:igem parts registry; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:23654268
Comments: The sequence of this plasmid is updated from the GenBank entry for pDCAF3 (KC619530.1) to include an IS5 insertion that occurred in the promoter region of the caffeine demethylation genes during passaging of the strain and a base change in the sequence of the gst9 gene. This plasmid still functions as described in the publication for pDCAF3.
Vector constitutively expresses N-demethylase genes under the control of the BBa_J23100 promoter (http://parts.igem.org/Part:BBa_J23100).
N-demethylases are only functional when grown at 30°C and require iron supplementation in minimal media. See:
Summers, R. M., Louie, T. M., Yu, C.-L., Gakhar, L., Louie, K.C., and Subramanian, M. (2012) Novel, highly specific Ndemethylases enable bacteria to live on caffeine and related purine alkaloids. J. Bacteriol. 194, 2041−2049.
For the quantification of caffeine (or other methylxanthine) content it is recommended to grow cells in M9 minimal medium supplemented with 2 g/L glucose and 2 g/L casein (M9GC).
Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions.
Proper citation: RRID:Addgene_65220 Copy
Species: Synthetic
Genetic Insert: Splitposon w/ KanR, LacI & PTac
Vector Backbone Description: Vector Backbone:pSB1C3; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25080493
Proper citation: RRID:Addgene_59947 Copy
Species: Oryza sativa
Genetic Insert: OsMIR390-B/c
Vector Backbone Description: Backbone Size:9620; Vector Backbone:pMDC123; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25809382
Proper citation: RRID:Addgene_61466 Copy
Species: Discosoma sp.
Genetic Insert: monomeric red fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61649 Copy
Species: Auquorea victoria
Genetic Insert: enhanced yellow fluorescent protein
Vector Backbone Description: Backbone Marker:Cambia, Canberra, Australia; Backbone Size:6000; Vector Backbone:pCAMBIA1300; Vector Types:Plant Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:25390188
Proper citation: RRID:Addgene_61655 Copy
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:7300; Vector Backbone:pGBKT7; Vector Types:Yeast Expression; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61703 Copy
Vector Backbone Description: Backbone Size:11775; Vector Backbone:pEarleyGate-202; Vector Types:Plant Expression, BiFC assay; Bacterial Resistance:Chloramphenicol and Kanamycin
Defining Citation: PMID:19843313
Proper citation: RRID:Addgene_61705 Copy
Species: Mus musculus
Genetic Insert: Olig2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pLP-IRES2-EGFP; Vector Types:Mammalian Expression, Cre/Lox, acceptor vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: mOlig2 was transferred from the donor vector (pDNR-mOlig2, Addgene plasmid #49397) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
Proper citation: RRID:Addgene_49399 Copy
Species: Homo sapiens
Genetic Insert: Sox2
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:5500; Vector Backbone:pLP-IRES2-EGFP; Vector Types:Mammalian Expression, Cre/Lox, acceptor vector; Bacterial Resistance:Chloramphenicol and Kanamycin
Comments: hSox2 was transferred from the donor vector (pDNR-hSox2, Addgene plasmid #49389) to the acceptor vector using Cre-mediated recombination at the LoxP sites.
Proper citation: RRID:Addgene_49391 Copy
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