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Species: Synthetic
Genetic Insert: Endlinker/terminator Part 6
Vector Backbone Description: Vector Backbone:pLVC-ELT-6; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-ELT-6. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113990 Copy
Species: Synthetic
Genetic Insert: Super-folder GFP
Vector Backbone Description: Vector Backbone:pL0M-SC-sfGFP; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pL0M-SC-sfGFP. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113995 Copy
Species: Synthetic
Genetic Insert: IPTG-inducible promoter
Vector Backbone Description: Vector Backbone:pL0M-PU-pLac; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pL0M-PU-pLac. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113994 Copy
Species: Synthetic
Genetic Insert: IPTG-inducible T7RNAP promoter
Vector Backbone Description: Vector Backbone:pL0M-PU-pT7lacO; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pL0M-PU-pT7lacO. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113993 Copy
Species: Synthetic
Genetic Insert: Level 2 golden gate cloning vector
Vector Backbone Description: Vector Backbone:pLVC-P1-GG; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-P1-GG cloning site Lv2. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113981 Copy
Species: Synthetic
Genetic Insert: Kanamycin/neomycin
Vector Backbone Description: Vector Backbone:pLVC-P2-neo; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-P2-neo. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113982 Copy
Species: Synthetic
Genetic Insert: Endlinker/terminator Part 4
Vector Backbone Description: Vector Backbone:pLVC-ELT-4; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:30692983
Comments: pLVC-ELT-4. Backbone from Invitrogen for synthesised parts. Please visit https://www.biorxiv.org/content/early/2018/08/16/392423 for bioRxiv preprint.
Proper citation: RRID:Addgene_113988 Copy
Species: Homo sapiens
Genetic Insert: JARID2
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114443 Copy
Species: Homo sapiens
Genetic Insert: PALI1
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114442 Copy
Species: Homo sapiens
Genetic Insert: LCOR
Vector Backbone Description: Backbone Marker:Invitrogen; Vector Backbone:pCR8/GW/TOPO; Vector Types:Gateway Entry Cloning; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29628311
Proper citation: RRID:Addgene_114441 Copy
Species: Mus musculus
Genetic Insert: Microtubule binding domain
Vector Backbone Description: Vector Backbone:pBART27 R4R3; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:29739839
Comments: Addgene QC results discovered an IS1 inserted into the plasmid backbone. Please review the sequence for further information.
Proper citation: RRID:Addgene_115222 Copy
Species: Homo sapiens
Genetic Insert: PARK7C53A
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31536960
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115179 Copy
Species: Homo sapiens
Genetic Insert: PARK7E163K
Vector Backbone Description: Backbone Marker:Thermo Scientific; Backbone Size:2790; Vector Backbone:pENTR223; Vector Types:gateway cloning into appropriate destination vectors; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:31536960
Comments: Derived from HsCD00383508 from Harvard PlasmidID. Mutagenesis performed using overlap-extension PCR in combination with Restriction digestion/ligation.
Proper citation: RRID:Addgene_115181 Copy
Species: Synthetic
Genetic Insert: QQR ZFN
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: QQR ZFN reference:
Even-Faitelson L, Samach A, Melamed-Bessudo C, Avivi-Ragolsky N, Levy AA (2011) Localized egg-cell expression of effector proteins for targeted modification of the Arabidopsis genome. The Plant Journal 68 (5):929-937. doi:10.1111/j.1365-313X.2011.04741.x
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
Proper citation: RRID:Addgene_44463 Copy
Species: Synthetic
Genetic Insert: AtCRU3 T494 TALENs
Vector Backbone Description: Backbone Size:13812; Vector Backbone:pHEX2; Vector Types:Plant Transformation; Bacterial Resistance:Spectinomycin
Comments: pZHY013 reference:
Zhang Y, Zhang F, Li X, Baller JA, Qi Y, Starker CG, Bogdanove AJ, Voytas DF (2012) TALENs enable efficient plant genome engineering. Plant Physiology. doi:10.1104/pp.112.205179
Please refer to the references provided below for matters relating to ownership of pHEX2 for anything other than academic use.
pHEX2 reference:
Hellens R, Allan A, Friel E, Bolitho K, Grafton K, Templeton M, Karunairetnam S, Gleave A, Laing W (2005) Transient expression vectors for functional genomics, quantification of promoter activity and RNA silencing in plants. Plant Methods 1 (1):13. doi:10.1186/1746-4811-1-13
pART27 (used to derive pHEX2) reference:
Gleave AP (1992) A versatile binary vector system with a T-DNA organisational structure conducive to efficient integration of cloned DNA into the plant genome. Plant Mol Biol 20:1203-1207
Proper citation: RRID:Addgene_44464 Copy
Species: Synthetic
Genetic Insert: Citrine-PTS1
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:10404; Vector Backbone:pMpGWB102; Vector Types:Plant Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23421791
Proper citation: RRID:Addgene_44592 Copy
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pCDFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_41135 Copy
Genetic Insert: MBP
Vector Backbone Description: Backbone Marker:Merck; Vector Backbone:pCDFduet-1; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin
Proper citation: RRID:Addgene_41134 Copy
Genetic Insert: LacZ + BsaI restriction sites
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2447; Vector Backbone:pCR8; Vector Types:; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:23388034
Comments: For more information on Yamamoto TALEN Add-On Plasmids please refer to: http://www.addgene.org/TALEN/Yamamotolab/
Proper citation: RRID:Addgene_43849 Copy
Species: Homo sapiens
Genetic Insert: USP44
Vector Backbone Description: Backbone Size:9600; Vector Backbone:pDEST-MYC; Vector Types:Mammalian Expression; Bacterial Resistance:Spectinomycin
Defining Citation: PMID:17443180
Proper citation: RRID:Addgene_43901 Copy
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