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Genetic Insert: dCas9
Vector Backbone Description: Vector Backbone:pCAH01SpR; Vector Types:CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:42030398
Comments: Please visit https://doi.org/10.1101/2025.05.28.656465 for bioRxiv preprint.
Proper citation: RRID:Addgene_251729 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: NrdJ-1C - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUTI site and fused to the C-terminal part of the split intein NrdJ-1.When co-expressed with pIN086 and pIN200, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255795 Copy
Species: Xenorhabdus nematophila ATCC 19061
Genetic Insert: SspGyrBC - XtpS
Vector Backbone Description: Vector Backbone:pCDF; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Comments: This plasmid encodes the termination unit of the non-ribosomal peptide synthetase (NRPS) called xenotetrapeptide synthetase (XtpS), split at an XUC site and fused to the C-terminal part of the split intein SspGyrB.When co-expressed with pIN097 and pIN202, the three fragments splice into a functional NRPS that produces the non-ribosomal peptide (NRP) called xenotetrapeptide (XTP; cyclo[Leu-D-Val-Val-D-Val]; CC(C)C[C@H]1C(=O)N[C@@H](C(=O)N[C@H](C(=O)N[C@@H](C(=O)N1)C(C)C)C(C)C)C(C)C; 410.6 g/mol).
For successful NRP production, the three plasmids must be co-transformed into an E. coli strain that expresses a broad-spectrum phosphopantetheinyl transferase, such as MtaA (DH10B::mtaA) or Sfp (BAP1), which is required for the post-translational activation of the T domains.
Expression is controlled by an araBAD promoter, inducible with 0.2% arabinose.
Proper citation: RRID:Addgene_255797 Copy
Vector Backbone Description: Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:modified pTF505; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:35690588
Proper citation: RRID:Addgene_187875 Copy
Vector Backbone Description: Backbone Marker:Keunsub Lee; Backbone Size:6149; Vector Backbone:modified pTF505; Vector Types:Bacterial Expression, CRISPR; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:35690588
Proper citation: RRID:Addgene_187874 Copy
Genetic Insert: eGFP
Vector Backbone Description: Backbone Marker:Ephraim Aliu; Backbone Size:6494; Vector Backbone:pEA106; Vector Types:Bacterial Expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:35690588
Proper citation: RRID:Addgene_187873 Copy
Species: S. elongatus PCC7942
Genetic Insert: synpcc7942_1377 (spoT)
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29241543
Proper citation: RRID:Addgene_100915 Copy
Species: E. coli
Genetic Insert: RelA fragment from E.coli
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29241543
Proper citation: RRID:Addgene_100916 Copy
Species: S. elongatus PCC7942
Genetic Insert: synpcc7942_1377 (spoT)
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:29241543
Proper citation: RRID:Addgene_100914 Copy
Species: S. elongatus PCC7942
Genetic Insert: kaiBC S431A T432A with p0050 promoter
Vector Backbone Description: Backbone Size:5700; Vector Backbone:pAM1303; Vector Types:Cyanobacteria cloning vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:28430105
Proper citation: RRID:Addgene_101837 Copy
Species: Synthetic
Genetic Insert: g3c
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30127387
Proper citation: RRID:Addgene_116893 Copy
Species: Synthetic
Genetic Insert: g3c
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30127387
Proper citation: RRID:Addgene_116891 Copy
Species: Synthetic
Genetic Insert: g3c
Vector Backbone Description: Vector Backbone:ColE1; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:30127387
Proper citation: RRID:Addgene_116892 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-S7942NS1-RK2BOM, CV-aadA_nt, CV-ccdB-SwaI.
Vector Backbone Description: Backbone Size:5008; Vector Backbone:S7942NS1-RK2BOM_aadA_nt_ccdB-SwaI; Vector Types:; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:25074377
Proper citation: RRID:Addgene_120084 Copy
Genetic Insert: pBS MCS
Vector Backbone Description: Backbone Size:6194; Vector Backbone:pAM4787; Vector Types:shuttle vector; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:27902432
Proper citation: RRID:Addgene_120086 Copy
Species: Synechococcus elongatus PCC 7942
Genetic Insert: Plasmid assembled from 4 CYANO-VECTOR modular devices: CV-pANS, CV-oribom, CV-aadA, PconII-LTRBS-yemGFP.
Vector Backbone Description: Backbone Size:7032; Vector Backbone:pBR322-aadA; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:27902432
Proper citation: RRID:Addgene_120088 Copy
Species: Synechocystis sp. PCC 6803 and Synechococcus elongatus PCC 7942
Genetic Insert: pAM4787 with pSYSA-TA1 cassette cloned into the SacI restriction site.
Vector Backbone Description: Backbone Size:7774; Vector Backbone:pAM4787; Vector Types:other; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:27902432
Proper citation: RRID:Addgene_120089 Copy
Species: Broad range of bacteria
Genetic Insert: Plasmid assembled from 3 CYANO-VECTOR modular devices: CV-RSF1010Y25F, CV-aadA, CV-PconII-LTRBS-GFPmut2
Vector Backbone Description: Backbone Size:8069; Vector Backbone:RSF1010Y25F_aadA_PconII-LTRBS-GFPmut2; Vector Types:Other; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:25074377
Proper citation: RRID:Addgene_120079 Copy
Species: Synthetic
Genetic Insert: turboGFP codon-optimised for plants (P. plumata) with intron, U5 small nuclear ribonucleoprotein component (A. thaliana)
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, turboGFP (P. plumata) codon-optimised for plants with intron, U5 small nuclear ribonucleoprotein component (A. thaliana) for transient and stable expression in plants. (Moved to CDS1 position from pICSL50016; Engler et al., 2014)
Proper citation: RRID:Addgene_123182 Copy
Species: Synthetic
Genetic Insert: luciferase gene from Renilla reniformis with an plant intron and codon optimized for plants
Vector Backbone Description: Backbone Marker:Weber et al., 2011 (DOI:10.1371/journal.pone.0016765); Backbone Size:2247; Vector Backbone:pICH41308; Vector Types:Plant Expression, Luciferase, part for plant expression; Bacterial Resistance:Spectinomycin and Streptomycin
Defining Citation: PMID:31263474
Comments: Golden Gate (MoClo - Weber et al., 2011), CDS1 position, Renilla luciferase with an intron for transient and stable expression in plants. Codon optimized for plants.
Proper citation: RRID:Addgene_123181 Copy
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