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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pFS16 Pmyo-3 NPP-9 mChBioFLAG Resource Report Resource Website |
RRID:Addgene_45099 | npp-9 | Caenorhabditis elegans | Ampicillin | PMID:22219512 | Backbone Marker:Promega; Backbone Size:2891; Vector Backbone:pGEM-T Easy; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:21 | 0 | ||
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pNH002 Resource Report Resource Website |
RRID:Addgene_42145 | N-TAP TAG::[(G4S)3]::mTFP1 | Caenorhabditis elegans | Ampicillin | PMID:23281894 | Nb. CDS terminates with two in-frame stop codons [TAA-TAG] | Backbone Marker:GeneOracle; Backbone Size:2172; Vector Backbone:pGOv5; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:51 | 0 | |
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pNH009 Resource Report Resource Website |
RRID:Addgene_42146 | N-TAP-tag::mTFP1 | Caenorhabditis elegans | Ampicillin | PMID:23281894 | Nb. CDS terminates with two in-frame stop codons [TAA-TAG] | Backbone Marker:GeneOracle; Backbone Size:2172; Vector Backbone:pGOv5; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:51 | 0 | |
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pNH013 Resource Report Resource Website |
RRID:Addgene_42147 | mTFP1 | Caenorhabditis elegans | Ampicillin | PMID:23281894 | Nb. CDS terminates with two in-frame stop codons [TAA-TAG] | Backbone Marker:GeneOracle; Backbone Size:2172; Vector Backbone:pGOv5; Vector Types:Synthetic Biology; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:51 | 0 | |
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pNH094 Resource Report Resource Website |
RRID:Addgene_42167 | mTFP1-RT-::[(G4S)3]::C-TAP Tag | Caenorhabditis elegans | Chloramphenicol and Tetracycline | PMID:23281894 | Single-copy vector under non-induced condition. Copy-number-inducible with arabinose enables transient induction of copy-number for DNA isolation, etc. | Backbone Marker:Eipcentre; Backbone Size:8353; Vector Backbone:pCC1Fos; Vector Types:; Bacterial Resistance:Chloramphenicol and Tetracycline | 2026-08-15 01:14:51 | 0 | |
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pEG268 Resource Report Resource Website |
RRID:Addgene_40075 | Mex-5 (S458A) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Alternate plasmid name: pMAL c2X (GW) + MEX-5 (S458A) | Backbone Marker:New England Biolabs (Modified); Backbone Size:6700; Vector Backbone:pMal c2X (GW); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S458A | 2026-08-15 01:14:33 | 0 |
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pEG593 Resource Report Resource Website |
RRID:Addgene_40119 | Mex-5 (ATG through 648 bp of 3'UTR) R274E K318E (RNAi res) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: pCG150 + 4.4kb+Dendra+ gen MEX-5 R274E K318E (RNAi res) +3’UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | R274E, K318E, Exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:33 | 0 |
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pEG607 Resource Report Resource Website |
RRID:Addgene_40193 | Mex-5 | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen- Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. GFP::MEX-5 transgenes were constructed in the same way as Dendra::MEX-5 constructs except a pDONR201-GFP/TEV/FLAG entry clone was used and wild-type sequence of MEX-5 exon 2 was used. Alternate plasmid name: mex-5 prom::GFP/TEV/FLAG::MEX-5::mex-5 3'UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:14:34 | 0 | |
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pFM114 Resource Report Resource Website |
RRID:Addgene_40160 | par-3N+S251A | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-3N+S251A Note: During the Addgene quality control process, it was determined that this construct originally encoded a par-3 fragment containing amino acid residues 1 through 408, but a premature stop codon limits the protein to amino acid residue 364. | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N terminal portion of protein (aa 1-364); S251A | 2026-08-15 01:14:34 | 0 |
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pFM115 Resource Report Resource Website |
RRID:Addgene_40161 | par-3C | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-3C | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | C terminal portion of protein (aa850-1286) | 2026-08-15 01:14:34 | 0 |
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pFM113 Resource Report Resource Website |
RRID:Addgene_40159 | par-3N | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-3N Note: During the Addgene quality control process, it was determined that this construct originally encoded a par-3 fragment containing amino acid residues 1 through 408, but a premature stop codon limits the protein to amino acid residue 364. | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | N terminal portion of protein (aa1-364) | 2026-08-15 01:14:34 | 0 |
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pFM112 Resource Report Resource Website |
RRID:Addgene_40158 | par-1C:FLAG | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-1C:FLAG | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | Contains aa 966-1192 | 2026-08-15 01:14:34 | 0 |
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pFM108 Resource Report Resource Website |
RRID:Addgene_40154 | par-2 RNAi resistant, S241A | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2RR S241A | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | S241A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM107 Resource Report Resource Website |
RRID:Addgene_40153 | par-2 RNAi resistant, 7SA | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2 7SA 7SA mutations are: S190A, S197A, S241A, S265A, S295A, S301A, and S335A. | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 7SA; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM106 Resource Report Resource Website |
RRID:Addgene_40152 | par-2 RNAi resistant, R163A | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2RR R163A | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | R163A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM105 Resource Report Resource Website |
RRID:Addgene_40151 | par-2 RNAi resistant, K162A | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pGEX6p1-par-2RR K162A | Backbone Marker:GE; Backbone Size:4900; Vector Backbone:pGEX-6p-1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | K162A; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM052 Resource Report Resource Website |
RRID:Addgene_40146 | par-2 RNAi resistant (C56S) | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pID3.01-par-2RR C56S | Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | C56S; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:34 | 0 |
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pFM038 Resource Report Resource Website |
RRID:Addgene_40141 | par-2 RNAi resistant (R183-5A + 7SA) | Caenorhabditis elegans | Ampicillin | PMID:21983565 | Alternate plasmid name: pID3.01-par-2RR R183-5A + 7SA 7SA mutations are: S190A, S197A, S241A, S265A, S295A, S301A, and S335A Note: A deletion of amino acid residues 502-523 near the C-terminus of the protein was discovered during the Addgene quality control process and is present in this plasmid. | Backbone Marker:Addgene plasmid 22720; Backbone Size:18033; Vector Backbone:pID3.01; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | R183-5A + 7SA; base pairs 6-523 of par-2 were recoded to create the RNAi-resistant par-2 transgene. | 2026-08-15 01:14:33 | 0 |
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pEG634 Resource Report Resource Website |
RRID:Addgene_40121 | Mex-5 (aa244-468) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: pCG150 + 4.4kb+Dendra+ gen MEX-5 aa245-STOP (RNAi res) +3’UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | contains aa244-468 | 2026-08-15 01:14:33 | 0 |
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pEG628 Resource Report Resource Website |
RRID:Addgene_40120 | Mex-5 (aa1-244) (RNAi resistant) | Caenorhabditis elegans | Ampicillin | PMID:21925318 | Dendra::MEX-5 constructs were constructed as follows. A 4.4 kb mex-5 promoter fragment based on Tenlen et al. (2008) was cloned into pDONRP4P1R (Invitrogen). Dendra2/TEV/S-peptide (Gallo et al., 2010) was cloned into pDONR201. A MEX-5 genomic fragment from the start ATG through 648 bp of 3'UTR was cloned into pDONRP2RP3. Exon 2 of mex-5 was recoded to be RNAi-resistant (Gen-Script) so as to allow depletion of endogenous MEX-5/6 without depletion of the transgene. These constructs were assembled into pCG150 using three-way Gateway system (LR reaction) (Invitrogen) (Merritt et al., 2008). Mutations were made by recombinant PCR and all inserts were sequenced verified. Alternate plasmid name: pCG150 + 4.4kb+Dendra+ gen MEX-5 aa1-245 (RNAi res) +3’UTR | Backbone Marker:Addgene plasmid 17247; Backbone Size:6351; Vector Backbone:pCG150; Vector Types:Worm Expression, Gateway Cloning; Bacterial Resistance:Ampicillin | contains aa1-244, Exon 2 recoded to be RNAi-resistant | 2026-08-15 01:14:33 | 0 |
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