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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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aav-CAG-FLEX-tdNfsB(F124W)-mCherry (eNTR) Resource Report Resource Website |
RRID:Addgene_113762 | tdNfsB(F124W)-mCherry | E. coli | Ampicillin | PMID:30111097 | Sternson Lab recommendations for propagation of AAV-FLEX plasmids: "These vectors are prone to recombination. This is a well known issue with these AAV vectors and is due to the inverted terminal repeats (ITRs) required for AAV production. To minimize recombination, we propagate these plasmids in NEB Stable cells. Also, to minimize recombination, cells should be cultured at 30 C. Note that these cultures will grow slowly (20 h for minipreps). Better yields and culture times are obtained with 2xYT as the media. This is strongly recommended. Because recombination may still happen occasionally, we do a panel of restriction digestions to assess whether the ITRs are in tact. Separate digestions with PvuII, Sma1, and SnaB1 should be performed." | Backbone Marker:Scott Sternson; Vector Backbone:AAV2; Vector Types:Mammalian Expression, AAV, Cre/Lox; Bacterial Resistance:Ampicillin | F124W | 2026-08-15 01:02:15 | 0 |
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pET29b-clbO Resource Report Resource Website |
RRID:Addgene_114153 | clbO | E. coli | Kanamycin | PMID:26890481 | Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:19 | 0 | ||
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pET28a-clbK Resource Report Resource Website |
RRID:Addgene_114152 | clbK | E. coli | Kanamycin | PMID:26890481 | Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:20 | 0 | ||
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pET28a-clbH-C-A2-PCP Resource Report Resource Website |
RRID:Addgene_114157 | clbH | E. coli | Kanamycin | PMID:28805802 | Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Only contains the C-A2-PCP domains of ClbH | 2026-08-15 01:02:19 | 0 | |
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pET28a-clbB Resource Report Resource Website |
RRID:Addgene_114155 | clbB | E. coli | Kanamycin | PMID:28805802 | Vector Backbone:pET28a; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:20 | 0 | ||
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pET29b-clbD Resource Report Resource Website |
RRID:Addgene_114146 | ClbD | E. coli | Kanamycin | PMID:26890481 | Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:19 | 0 | ||
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pET29b-clbF Resource Report Resource Website |
RRID:Addgene_114148 | ClbF | E. coli | Kanamycin | PMID:26890481 | Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:19 | 0 | ||
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pET29b-clbE Resource Report Resource Website |
RRID:Addgene_114147 | ClbE | E. coli | Kanamycin | PMID:26890481 | Vector Backbone:pET29b; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:19 | 0 | ||
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pSKB3.EG10680 Resource Report Resource Website |
RRID:Addgene_11436 | OsmC | E. coli | Kanamycin | PMID:15103136 | Please note that the plasmid pSKB3.EG10680 contains a Kanamycin resistance marker, and the BL21 bacterial strain contains a plasmid, pSJS1244, that confers spectinomycin resistance and allows for expression of genes with rare codons. Addgene provides this plasmid in the DH5alpha bacterial strain. For expression, please use BL21(DE3).Star/pSJS1244 | Backbone Size:5386; Vector Backbone:pSKB3; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:22 | 0 | |
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pcDNA3.1 mycBioID Resource Report Resource Website 50+ mentions |
RRID:Addgene_35700 | BirA | E. coli | Ampicillin | PMID:22412018 | Backbone Marker:InVitrogen; Backbone Size:5400; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | R118G (highly promiscuous form) | 2026-08-15 01:14:03 | 93 | |
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pcDNA3.1 MCS-BirA(R118G)-HA Resource Report Resource Website 50+ mentions |
RRID:Addgene_36047 | BirA(R118G)-HA | E. coli | Ampicillin | PMID:22412018 | Backbone Marker:Invitrogen; Backbone Size:5333; Vector Backbone:pcDNA3.1; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | R118G (highly promiscuous form) | 2026-08-15 01:14:08 | 64 | |
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pBEST-p15A-PLlacO1-lacI-T500 Resource Report Resource Website |
RRID:Addgene_45393 | lac repressor | e. coli | Ampicillin | PMID:20576148 | Backbone Marker:Promega; Backbone Size:4486; Vector Backbone:pBEST-Luc; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:23 | 0 | ||
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L5-LexAp65-L2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_41437 | LexAp65 | E. coli | Kanamycin | Backbone Marker:Invitrogen; Backbone Size:2551; Vector Backbone:pDONR P5-P2; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:45 | 1 | |||
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pCAG-AILR-LplA-ER Resource Report Resource Website |
RRID:Addgene_42574 | LplA(AILR) with ER-retention sequence KDEL | E. coli | Ampicillin | PMID:23181687 | Backbone Size:4261; Vector Backbone:pCAG; Vector Types:; Bacterial Resistance:Ampicillin | Compared to wild-type LplA: tryptophan 37 to alanine; threonine 57 to isoleucine; phenylalanine 147 to leucine; histidine 267 to arginine | 2026-08-15 01:14:55 | 0 | |
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pEIAV-SIN6.1 CZW Resource Report Resource Website |
RRID:Addgene_44172 | LacZ | E. coli | Ampicillin | PMID:16397505 | Backbone Marker:Promega Corporation; Backbone Size:2700; Vector Backbone:pCI; Vector Types:Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:13 | 0 | ||
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pEIAV-SIN6.1 CBLacZ-LucWm1A Resource Report Resource Website |
RRID:Addgene_44167 | LacZ | E. coli | Ampicillin | PMID:23319179 | Backbone Marker:Promega Corporation; Backbone Size:2700; Vector Backbone:pCI; Vector Types:Lentiviral, Luciferase; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:13 | 0 | ||
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pSCC2 Resource Report Resource Website |
RRID:Addgene_39993 | EcoRI endonuclease & EcoRI methylase | E. coli | Ampicillin | PMID:6088551 | pKC30 plasmid derivation is described in Nature (1981) 292, 128-132. Please note that this plasmid may require a unique bacterial strain, so make sure to confirm that you can also obtain the appropriate growth strain. Please contact us at [email protected] or contact our distributors if you have any questions. | Backbone Marker:n/a; Backbone Size:3000; Vector Backbone:pKC30; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | none | 2026-08-15 01:14:32 | 0 |
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pLMB51 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40083 | gusA | E. coli | Tetracycline | PMID:22820336 | GenBank accession number JQ895026 Multiple-cloning site (MCS) of pLMB51: GATTACCTCA GTCTAGAGCT CGAGAAGCTT GGATCCATGG TACCCTACA | Vector Backbone:RP4; Vector Types:Bacterial Expression; Bacterial Resistance:Tetracycline | 2026-08-15 01:14:33 | 1 | |
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pAT253 Resource Report Resource Website 1+ mentions |
RRID:Addgene_40943 | GFP-LacI** | E. coli | Ampicillin | PMID:21724830 | The plasmid pAFS135 (Straight, Sedat, and Murray, 1998) contains GFP(S65T, V163A)-LacI(P3Y, del C-terminal 11 aa)-SV40 NLS under the control of a HIS3 promoter fragment. To construct pAT123, the HIS3 marker of AFS135 was replaced with a ScaI-DraIII fragment from RS405 containing LEU2 (a kind gift from Karine Dubrana and Susan Gasser). The plasmid pAT222 (Addgene plasmid #40942) was obtained by site-directed mutagenesis of pAT123 to create GFP-LacI**, using the primers (base mutations in lowercase): 5’- GTGGCACAgCAACTGGCGGaCAAAggtggcggaTCGTTGCTGATTGGCGTTGCCtCCT-3’ and 5’- AGGaGGCAACGCCAATCAGCAACGAtccgccaccTTTGtCCGCCAGTTGcTGTGCCAC- 3’. The LacI** variant was then subcloned into pAFS135 using NotI and XhoI restriction sites to create plasmid pAT253. | Backbone Marker:Andrew W. Murray Lab, UCSF (Straight, Sedat, & Murray, 1998. PMID: 9813090); Backbone Size:4600; Vector Backbone:pAFS135; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin | LacI**/LacI2G(4 mutations) (see comment below); lacI-I12 mutation (P3Y); C-terminal 11 amino acids deleted to prevent tetramerization | 2026-08-15 01:14:40 | 1 |
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pHL1561 Resource Report Resource Website |
RRID:Addgene_37571 | CsrA | E. coli | Kanamycin | PMID:23878244 | Backbone Marker:Lim Lab; Vector Backbone:pZ with p15a origin; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Kanamycin | 2026-08-15 01:14:15 | 0 |
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