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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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Paxillin_PSCFP2_ mEOS2 Resource Report Resource Website |
RRID:Addgene_114192 | Paxillin | Gallus gallus | Kanamycin | Vector Backbone:pmEOS2-Paxillin; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:02:20 | 0 | |||
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pBa.NgCam.GFP Resource Report Resource Website |
RRID:Addgene_45061 | NgCam | Gallus gallus | Ampicillin | PMID:10839364 | NgCam is very GC rich and difficult to sequence and PCR. pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains. Addgene's sequencing results found a few nucleotide differences when compared to the full plasmid sequence provided by the depositing laboratory. According to the depositor, these differences are not a concern for the function of the plasmid. | Backbone Size:6755; Vector Backbone:pBa-eGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:20 | 0 | |
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pCAGGS-Hoxa7 Resource Report Resource Website |
RRID:Addgene_45554 | Hoxa7 | Gallus gallus | Ampicillin | PMID:23359544 | Backbone Size:4800; Vector Backbone:pCAGGs; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:25 | 0 | ||
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TN-XXL pRSETB Resource Report Resource Website |
RRID:Addgene_45796 | TN-XXL | Gallus gallus | Ampicillin | PMID:19160515 | TN-XXL is composed of two fluorescent proteins: cyan fluorescent protein (CFP) as the FRET donor and cpCitrine as the FRET acceptor. These proteins are linked by the calcium-sensitive double C-terminal lobe of troponin C (TnC). After the binding of free calcium, TnC undergoes a reversible conformational change that leads to energy transfer from the donor to the acceptor fluorophore, resulting in a drop in CFP fluorescence and an increase in cpCitrine fluorescence. | Backbone Marker:Invitrogen; Backbone Size:2900; Vector Backbone:pRSETB; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:26 | 0 | |
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pET15b/GgVcl 1-1066 (full length) Resource Report Resource Website 1+ mentions |
RRID:Addgene_46171 | Vcl | Gallus gallus | Ampicillin | PMID:15195105 | Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:29 | 1 | ||
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pEXP5-NT-AlleyCat Resource Report Resource Website |
RRID:Addgene_41715 | mutated C-terminal portion of calmodulin gene (M76-K148) | Gallus gallus | Ampicillin | PMID:21482808 | Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | F92E | 2026-08-15 01:14:47 | 0 | |
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pEXP5-NT-7th R Resource Report Resource Website |
RRID:Addgene_42573 | mutated calmodulin gene | Gallus gallus | Ampicillin | PMID:23630096 | Backbone Marker:Invitrogen; Backbone Size:2745; Vector Backbone:pEXP5-NT/TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | changed Phenylalanine 92 to Glutamine acid, Isoleucine 85 to Leucine, Alanine 88 to Glutamine, Histidine 107 to Isoleucine, Leucine 112 to Arginine, Methionine 124 to Leucine, Alanine 128 to Threonine, Methionine 144 to Arginine | 2026-08-15 01:14:55 | 0 | |
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pET15b/ECFP-GgVcl 884-1066 (Vt) Resource Report Resource Website 1+ mentions |
RRID:Addgene_46180 | Vcl 884-1066 | Gallus gallus | Ampicillin | CFP subcloned into 15b/V884-1066 (Addgene plasmid 46176) via a PCR strategy which placed Nde sites at the ends of the CFP cDNA and added the spacer region in EGFPC1/V1-1066 at the Cterminus of CFP. | Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | aa 884-1066 (tail domain) | 2026-08-15 01:15:29 | 1 | |
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pET15b/EYFP-GgVcl 884-1066-ECFP FRET Resource Report Resource Website |
RRID:Addgene_46179 | Vcl 884-1066 | Gallus gallus | Ampicillin | Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | aa 884-1066 (Vt, tail domain) | 2026-08-15 01:15:30 | 0 | ||
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pET15b/GgVcl V1-851 (aka Vh) Resource Report Resource Website |
RRID:Addgene_46172 | Vcl 1-851 | Gallus gallus | Ampicillin | PMID:15728584 | Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site. | Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | aa 1-851 (aka Vh) head domain | 2026-08-15 01:15:30 | 0 |
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pET15b/GgVcl V1-258 (aka VD1) Resource Report Resource Website |
RRID:Addgene_46173 | Vcl 1-258 | Gallus gallus | Ampicillin | PMID:15728584 | Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site. | Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | aa 1-258 (VD1) | 2026-08-15 01:15:29 | 0 |
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pET15b/GgVcl 884-1066 (aka Vt) Resource Report Resource Website |
RRID:Addgene_46176 | Vcl 884-1066 | Gallus gallus | Ampicillin | PMID:10373448 | Using in-frame primers containing 5′-NdeI and 3′-XhoI sites, appropriate regions of the chicken vinculin cDNA sequence were amplified by PCR followed by addition of 3′-adenosine overhangs with Taq polymerase. The PCR products were ligated into the TOPO II plasmid (Invitrogen) and then subcloned into pET15b His tag expression vector (Novagen, Madison, WI) using NdeI and XhoI digestion of the multiple cloning site. | Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | aa 884-1066 (tail domain) | 2026-08-15 01:15:29 | 0 |
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pET15b/GgVcl 1-851 A50I mutation Resource Report Resource Website |
RRID:Addgene_46174 | Vcl 1-851 A50I | Gallus gallus | Ampicillin | PMID:16608855 | See Addgene plasmid 46172 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene). | Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | aa 1-851 A50I mutation (talin binding domain mutant in vinculin head domain) | 2026-08-15 01:15:29 | 0 |
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pET15b/GgVcl 1-258 A50I mutation Resource Report Resource Website |
RRID:Addgene_46175 | Vcl 1-258 A50I | Gallus gallus | Ampicillin | PMID:16608855 | See Addgene plasmid 46173 for wild-type cloning information. The A50I mutation was introduced by QuikChange mutagenesis (Stratagene). | Backbone Marker:EMD; Backbone Size:5700; Vector Backbone:pET-15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin | aa 1-258 (VD1) A50I mutation; talin binding deficient | 2026-08-15 01:15:30 | 0 |
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pmCIT-CFPGgVcl 1-419 control 2 FRET Resource Report Resource Website |
RRID:Addgene_46283 | ECFP + Vcl 1-419 | Gallus gallus | Kanamycin | PMID:17074767 | Citrine proved to be a more stable FP in vivo than YFP; CIT was used to make a second generation vinculin FRET probe (first generation control is Addgene plasmid 46277). | Backbone Marker:Craig lab; Vector Backbone:pmCitrine-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | aa 1-419 | 2026-08-15 01:15:30 | 0 |
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pmECFPN3/GgVcl 1-1066 (CFP at C term) Resource Report Resource Website |
RRID:Addgene_46280 | Vcl 1-1066 | Gallus gallus | Kanamycin | PMID:15883197 | A206K was introduced into pECFP-N3/V1-1066 by QC to generate pmECFP/V1066 (vinculin-monomeric ECFP) | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pECFP-N3; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 0 | |
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pmECFPC1/GgVcl 1-1066 (CFP at N term) Resource Report Resource Website |
RRID:Addgene_46281 | Vcl 1-1066 | Gallus gallus | Kanamycin | PMID:15883197 | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pECFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 0 | ||
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p401F/GgVcl 1-1066 (N term FLAG tag) Resource Report Resource Website |
RRID:Addgene_46284 | Vcl 1-1066 | Gallus gallus | Ampicillin | Backbone Marker:described in N. Kioka. 1999. JCB 144:59-69; Vector Backbone:p401F; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:15:30 | 0 | |||
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pEYFPC1/GgVcl 1-1066 (YFP at N term) Resource Report Resource Website |
RRID:Addgene_46279 | Vcl 1-1066 | Gallus gallus | Kanamycin | PMID:15883197 | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEYFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 2026-08-15 01:15:30 | 0 | ||
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pEGFPC1/GgVcl 1-258 A50I mutation Resource Report Resource Website 1+ mentions |
RRID:Addgene_46271 | Vcl 1-258 (aka VD1) A50I mutation | Gallus gallus | Kanamycin | PMID:16608855 | A50I mutation introduced into EGFP/V1-258 (Addgene plasmid 46270) by QCPCR. | Backbone Marker:Clonetech; Backbone Size:4700; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin | 1-258 (VD1 domain) A50I mutation | 2026-08-15 01:15:30 | 1 |
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