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  • Organism:saccharomyces cerevisiae (facet)


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Plasmid Name Proper Citation Insert Name Organism Bacterial Resistance Defining Citation Comments Vector Backbone Description Relevant Mutation Record Last Update Mentions Count
pJLI-SUP35 (delta)2-5
 
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RRID:Addgene_1138 sup35 delta 2-5 mutant, 5' and 3' flanking regions Saccharomyces cerevisiae Ampicillin PMID:10448860 Backbone Size:4299; Vector Backbone:pRS306; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin last four repeats of sup35 N domain deleted, but rest of ORF intact. Also includes sup35 5' and 3' flanking regions 2026-08-15 01:02:15 0
pURD214
 
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RRID:Addgene_113871 sgRNA5 HXT13-15-16 sgRNA2-HXT2 Saccharomyces cerevisiae Ampicillin PMID:30285096 Backbone pROS10 is described in ""CRISPR/Cas9: a molecular Swiss army knife for simultaneous introduction of multiple genetic modifications in Saccharomyces cerevisiae. Mans R, van Rossum HM, Wijsman M, Backx A, Kuijpers NG, van den Broek M, Daran-Lapujade P, Pronk JT, van Maris AJ, Daran JM. FEMS Yeast Res. 2015 Mar;15(2). pii: fov004. doi: 10.1093/femsyr/fov004. Epub 2015 Mar 4. 10.1093/femsyr/fov004 PubMed 25743786 Backbone Marker:Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov004; Backbone Size:5577; Vector Backbone:pROS10 (#107924); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:02:16 0
pUDR418
 
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RRID:Addgene_113875 doble sgRNA11-STL1 Saccharomyces cerevisiae Ampicillin PMID:30285096 Backbone Marker:Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov004; Backbone Size:5577; Vector Backbone:pROS10 (#107924); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:02:16 0
pUDR220
 
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RRID:Addgene_113873 sgRNA8-HXT10 sgRNA7-HXT9-11-12 Saccharomyces cerevisiae Ampicillin PMID:30285096 Backbone Marker:Mans et al FEMS Yeast Res. 2015 Mar;15(2). pii: fov004; Backbone Size:6632; Vector Backbone:pROS14 (#107928); Vector Types:Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin 2026-08-15 01:02:16 0
pRS406 ATG9-GFP T119D
 
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RRID:Addgene_114668 ATG9 Saccharomyces cerevisiae Ampicillin PMID:27050455 Backbone Size:4297; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:24 0
pRS406 ATG9 stopGFP
 
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RRID:Addgene_114669 ATG9 Saccharomyces cerevisiae Ampicillin PMID:27050455 Backbone Size:4297; Vector Backbone:pRS406; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Changed the three linker base pairs after the last Arginine to stop codon TAA 2026-08-15 01:02:24 0
pGALSc104(A503V/A509D)
 
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RRID:Addgene_1148 HSP104 Saccharomyces cerevisiae Ampicillin PMID:14978213 Backbone Size:5703; Vector Backbone:pLA1 (pRS313:GAL1-10); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin A503V/A509D 2026-08-15 01:02:27 0
pGALSc104(G217S)
 
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1+ mentions
RRID:Addgene_1149 HSP104 Saccharomyces cerevisiae Ampicillin PMID:14978213 Backbone Size:5703; Vector Backbone:pLA1 (pRS313:GAL1-10); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin G217S 2026-08-15 01:02:28 1
pGALSc104(G217S/T499I)
 
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1+ mentions
RRID:Addgene_1151 HSP104 Saccharomyces cerevisiae Ampicillin PMID:14978213 Backbone Size:5703; Vector Backbone:pLA1 (pRS313:GAL1-10); Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin G217S/T499I 2026-08-15 01:02:31 1
104b-U1(WT)
 
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1+ mentions
RRID:Addgene_1153 HSP104 Saccharomyces cerevisiae Ampicillin PMID:14978213 Backbone Size:4895; Vector Backbone:pRS316; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:02:32 1
pRS424GFP-2xPH(Osh2)
 
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1+ mentions
RRID:Addgene_36095 2xPH(Osh2) fusion Saccharomyces cerevisiae Ampicillin PMID:21295699 Please note that the first Osh2 domain contains aa273-421 and the second Osh2 domains contains aa260-421 when compared to GenBank reference sequence NP_010265.1. According to this reference sequence the PH domain is aa 292–384. The additional residues surrounding the PH domain were intentionally cloned into this construct. Vector Backbone:pRS424; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:05 2
pCY 3010-02
 
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RRID:Addgene_36210 yeast-enhanced (yE) polylinker Saccharomyces cerevisiae Ampicillin PMID:22473760 Backbone Marker:Yeast Resource Center, University of Washington; Backbone Size:4875; Vector Backbone:pBS10; Vector Types:Yeast cassette for PCR and homologous recombination; Bacterial Resistance:Ampicillin 2026-08-15 01:14:06 0
pNRB65
 
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RRID:Addgene_36455 ARS501-LEU2 Saccharomyces cerevisiae Ampicillin This plasmid is for generating dmc1Δ::LEU2 alleles by single step transplacement. See the attached map for additional details. Backbone Size:3000; Vector Backbone:pNRB8; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:08 0
pJJH1308
 
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RRID:Addgene_36911 ScURA3 Saccharomyces cerevisiae Ampicillin PMID:22327349 Vector Backbone:L22; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:11 0
pJJH1338
 
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RRID:Addgene_36912 ScURA3 Saccharomyces cerevisiae Ampicillin PMID:22327349 Vector Backbone:L22-EGFP; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression, Lentiviral; Bacterial Resistance:Ampicillin 2026-08-15 01:14:11 0
pJJH1295
 
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1+ mentions
RRID:Addgene_36914 3x mCherry Saccharomyces cerevisiae Ampicillin PMID:22327349 Backbone Size:9951; Vector Backbone:pSinRep5; Vector Types:Mammalian Expression, Bacterial Expression, Yeast Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:11 2
pBJ1808 pRS416-COF1RFP
 
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RRID:Addgene_37103 COF1-RFP Saccharomyces cerevisiae Ampicillin PMID:20332110 Vector Backbone:pRS416-URA,Amp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Single RFP internal insertion at Cof147T48D with linker(AGAGDGAGL) at both sides. This construct contains endogenous COF1 5' 650 , intron, and 3' 250 bp. Cof1-RFP labels actin patches in yeast. 2026-08-15 01:14:12 0
pBJ1806 pRS413-COF1RFP
 
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RRID:Addgene_37101 COF1-RFP Saccharomyces cerevisiae Ampicillin PMID:20332110 Vector Backbone:pRS413-HIS,Amp; Vector Types:Yeast Expression; Bacterial Resistance:Ampicillin Single RFP internal insertion at Cof147T48D with linker(AGAGDGAGL) at both sides. This construct contains endogenous COF1 5' 650 , intron, and 3' 250 bp. Cof1-RFP labels actin patches in yeast. 2026-08-15 01:14:12 0
pET15b-eIF4E
 
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RRID:Addgene_37228 eIF4E Saccharomyces cerevisiae Ampicillin PMID:20864040 Backbone Marker:EMD bioscience; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin 2026-08-15 01:14:13 0
pET15b-eIF4E-S200C
 
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RRID:Addgene_37229 eIF4E Saccharomyces cerevisiae Ampicillin PMID:20864040 Backbone Marker:EMD bioscience; Backbone Size:5700; Vector Backbone:pET15b; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin S200C 2026-08-15 01:14:13 0

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