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| Plasmid Name | Proper Citation | Insert Name | Organism | Bacterial Resistance | Defining Citation |
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pAAV-CaMKIIa-oChIEF(E163A/T199C)-P2A-mKate2 Resource Report Resource Website 1+ mentions |
RRID:Addgene_51092 | oChIEF(E163A/T199C) | Synthetic | Ampicillin | The addition of the E163A and T199C mutations in oChIEF impart higher photocurrent amplitude while retaining fast kinetic properties. This variant is proposed as an alternative to ChETA variants that exhibit smaller photocurrents and more toxicity. | Backbone Marker:custom; Backbone Size:5100; Vector Backbone:pAAV-CaMKIIa-WPRE-HGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | E163A/T199C | 2026-08-15 01:16:14 | 1 | |
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AAV-EF1a-DIO-ChIEF(E162A/T198C)-P2A-dTomato-WPRE-BGHpA Resource Report Resource Website |
RRID:Addgene_51095 | ChIEF(E162A/T198C) | Synthetic | Ampicillin | The addition of the E162A and T198C mutations in ChIEF impart higher photocurrent amplitude while retaining fast kinetic properties. This variant is proposed as an alternative to ChETA variants that exhibit smaller photocurrents and more toxicity. | Backbone Marker:custom; Backbone Size:1047; Vector Backbone:pAAV-EF1a-DIO-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | E162A/T198C | 2026-08-15 01:16:14 | 0 | |
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pICSL11024 (pICH47732::NOSp-NPTII-OCST) Resource Report Resource Website 10+ mentions |
RRID:Addgene_51144 | NOSp-NPTII-OCST | Synthetic | Ampicillin | Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH47732; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:14 | 22 | |||
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pICSL11017 (pICH47732::NOSp-BAR-NOST) Resource Report Resource Website 1+ mentions |
RRID:Addgene_51145 | NOSp-BAR-NOST | Synthetic | Ampicillin | Backbone Marker:Sylvestre Marillonnet; Vector Backbone:pICH47732; Vector Types:; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:11 | 1 | |||
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AAV-oChEF-citrine Resource Report Resource Website |
RRID:Addgene_50975 | ChEF-citrine | Synthetic | Ampicillin | PMID:19254539 | WPRE is inserted after the citrine before SV40 polyA. | Backbone Size:4599; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin | ChEF gene is mammalian codon optimized | 2026-08-15 01:16:10 | 0 |
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AAV-oChIEF-tdTomato Resource Report Resource Website 1+ mentions |
RRID:Addgene_50977 | ChIEF-tdTomato | Synthetic | Ampicillin | PMID:19254539 | Backbone Size:4599; Vector Backbone:AAV2; Vector Types:AAV; Bacterial Resistance:Ampicillin | ChIEF gene is mammalian codon optimized | 2026-08-15 01:16:13 | 8 | |
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pRS316-RGR-GFP-mHH Resource Report Resource Website |
RRID:Addgene_51057 | RGR-GFP | Synthetic | Ampicillin | PMID:24373158 | Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:13 | 0 | ||
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pRS316-RGR-GFP-mm Resource Report Resource Website |
RRID:Addgene_51059 | RGR-GFP | Synthetic | Ampicillin | PMID:24373158 | Backbone Size:4887; Vector Backbone:pRS316; Vector Types:Bacterial Expression, Yeast Expression, CRISPR; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:10 | 0 | ||
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AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato Resource Report Resource Website 10+ mentions |
RRID:Addgene_51083 | GCaMP6f | Synthetic | Ampicillin | The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled. Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid. | Backbone Marker:custom; Backbone Size:5320; Vector Backbone:pAAV-EF1a-DIO-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:11 | 10 | ||
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AAV-EF1a-DIO-GCaMP6s-P2A-nls-dTomato Resource Report Resource Website 10+ mentions |
RRID:Addgene_51082 | GCaMP6s | Synthetic | Ampicillin | The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled. Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid. | Backbone Marker:custom; Backbone Size:5320; Vector Backbone:pAAV-EF1a-DIO-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:14 | 10 | ||
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AAV-hSyn1-GCaMP6f-P2A-nls-dTomato Resource Report Resource Website 10+ mentions |
RRID:Addgene_51085 | GCaMP6f | Synthetic | Ampicillin | The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled. Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid. | Backbone Marker:Modified from Karl Deisseroth; Backbone Size:4590; Vector Backbone:pAAV-hSyn1-WPRE-HGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:14 | 17 | ||
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AAV-CaMKIIa-GCaMP6f-P2A-nls-dTomato Resource Report Resource Website 1+ mentions |
RRID:Addgene_51087 | GCaMP6f | Synthetic | Ampicillin | The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled. Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid. | Backbone Marker:custom; Backbone Size:5095; Vector Backbone:pAAV-CaMKIIa-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:11 | 6 | ||
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AAV-CaMKIIa-GCaMP6s-P2A-nls-dTomato Resource Report Resource Website 1+ mentions |
RRID:Addgene_51086 | GCaMP6s | Synthetic | Ampicillin | The addition of the P2A-nls-dTomato allows for easy identification of GCaMP6 expressing cells exhibiting nuclear red fluorescence that does not significantly overlap with the cytosolic GCaMP signal. The GCaMP and fluorophore are physically uncoupled. Permissions were obtained from Douglas Kim and the Clontech licensing office for depositing this new plasmid. | Backbone Marker:custom; Backbone Size:5095; Vector Backbone:pAAV-CaMKIIa-WPRE-BGHpA; Vector Types:Mammalian Expression, AAV; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:11 | 2 | ||
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pLenti.CAG.H2B-Dendra2.W Resource Report Resource Website 1+ mentions |
RRID:Addgene_51005 | H2B-Dendra2 | Synthetic | Ampicillin | Please note that the H2B sequence in this plasmid was derived from H2B-GFP (Addgene plasmid# 11680) and contains the same differences (D26G and V119I) compared to GenBank reference sequences, such as NP_066402.2. | Vector Backbone:pLenti.CAG; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:10 | 6 | ||
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3xFLAG-dCas9/pMXs-neo Resource Report Resource Website 1+ mentions |
RRID:Addgene_51260 | 3xFLAG-dCas9 | Synthetic | Ampicillin | PMID:25051498 | The coding sequence of 3xFLAG-dCas9 can be cleaved with Pac I and Not I. Construction strategy of gRNA retroviral vectors 1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I). 2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors. Vectors & Sites of insertion pSIR-neo (Addgene #51128): eg. [Xho I + Hind III] pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I pSIR-hCD2 (Addgene #51143): eg. EcoR I | Backbone Marker:Hodaka Fujii; Backbone Size:6238; Vector Backbone:pMXs-neo; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin | human codon-optimized, D10A + H840A | 2026-08-15 01:16:15 | 1 |
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Pyronic/pcDNA3.1(-) Resource Report Resource Website 10+ mentions |
RRID:Addgene_51308 | Pyronic | Synthetic | Ampicillin | PMID:24465702 | Backbone Marker:Invitrogen; Backbone Size:5419; Vector Backbone:pcDNA3.1(-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:12 | 15 | ||
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3xFLAG-dCas9/pMXs-puro Resource Report Resource Website 1+ mentions |
RRID:Addgene_51240 | 3xFLAG-dCas9 | Synthetic | Ampicillin | PMID:25051498 | The coding sequence of 3xFLAG-dCas9 can be cleaved with Pac I and Not I. Construction strategy of gRNA retroviral vectors 1. Cleave gBlock from a gRNA vector constructed using gRNA cloning vector (Addgene #41824) with appropriate restriction enzymes (eg. [Xho I + Hind III], EcoR I). 2. Insert the cleaved gBlock into pSIR-based self-inactivating retroviral vectors. Vectors & Sites of insertion pSIR-neo (Addgene #51128): eg. [Xho I + Hind III] pSIR-GFP (Addgene #51134): eg. [Xho I + Hind III], EcoR I pSIR-DsRed-Express2 (Addgene #51135): eg. [Xho I + Hind III], EcoR I pSIR-hCD2 (Addgene #51143): eg. EcoR I | Backbone Marker:Hodaka Fujii; Backbone Size:5791; Vector Backbone:pMXs-puro; Vector Types:Mammalian Expression, Retroviral, CRISPR; Bacterial Resistance:Ampicillin | human codon-optimized, D10A + H840A | 2026-08-15 01:16:12 | 6 |
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pcDNA3-mNeptune2 Resource Report Resource Website |
RRID:Addgene_51309 | mNeptune2 | Synthetic | Ampicillin | PMID:24633408 | Backbone Marker:Invitrogen; Backbone Size:5425; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:12 | 0 | ||
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pcDNA3-mNeptune2.5 Resource Report Resource Website 1+ mentions |
RRID:Addgene_51310 | mNeptune2.5 | Synthetic | Ampicillin | PMID:24633408 | Backbone Marker:Invitrogen; Backbone Size:5425; Vector Backbone:pcDNA3; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin | 2026-08-15 01:16:15 | 5 | ||
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2NQA Resource Report Resource Website |
RRID:Addgene_51330 | 2NQA | Synthetic | Kanamycin | http://www.thesgc.org/structures/2NQA/ | Vector Backbone:pET28a-LIC; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin | Truncation: See Comments | 2026-08-15 01:16:16 | 0 |
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