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SciCrunch Registry is a curated repository of scientific resources, with a focus on biomedical resources, including tools, databases, and core facilities - visit SciCrunch to register your resource.
https://cell-innovation.nig.ac.jp/maser/Tools/visualization_top_en.html
One stop platform for NGS big data from analysis to visualization. There are about 400 analysis pipelines integrated on Maser. List of all analysis pipelines, including descriptions and approximate execution times, can be found on page for ‘All pipelines’ in the User Guide.. Regist custom genome software registers custom genomes to Genome Explorer (IN: FASTA).
Proper citation: regist custom genome (RRID:SCR_015999) Copy
http://www.stowers-institute.org/research/core/molecular-biology
Core services include DNA sequencing, site directed mutagenesis, genome engineering (TALENs & CRISPRS), plasmid preparations and distributing clones/vectors from in house collections. Supports real time quantitative PCR through instrument training, troubleshooting and experimental design. Stowers researchers also have access to Illumina Next Generation Sequencing technology. Constructs libraries, performs sequencing and assists with high throughput genome sequencing, RNA-seq and ChIP-seq projects. Utilizes liquid handling and colony manipulation robots to automate many of services. Provides automation expertise and collaborate with researchers on custom automation projects.
Proper citation: Stowers Institute for Medical Research Molecular Biology Core Facility (RRID:SCR_017776) Copy
Core provides services and houses samples collected over the last 30 years. Biorepository processes, archives, and retrieves biological samples for genomic research. Offers variety of sample processing options including but not limited to automated DNA extraction using Autogen FLEXSTAR+ instrument, automated DNA/RNA extraction using Qiagen QIASymphony, peripheral blood mononuclear cell (PBMC) isolation, serum, plasma and buffy coat isolation, creation of blood cards, DNA/RNA quantitation and qualitation, whole genome amplification, cell-line lymphoblast immortalization and primary fibroblast tissue culture. Offers sample solutions tos uit customized needs.Services also include DNA/RNA Extraction,Sample archiving, retrieval and allocation,Unique and custom labels printing,DNA/RNA quantitation and qualitation,Tissue culture,Whole genome amplification.
Proper citation: University of Miami Hussman Institute for Human Genomics Biorepository Core Facility (RRID:SCR_017816) Copy
Core Facility offers services utilizing Illumina Novaseq X Plus, Pacific Biosciences Revio, ONT Promethion, and 10x Genomics platforms. The core has extensive knowledge of DNA/RNA library preparation for short-read, long-read and single cell sequencing. Sample preparation is fully automated on Perkin Elmer robotic workstations and tracked via the Clarity LIMS. Services include, but are not limited to, whole genome, exome and custom capture protocols, as well as, bulk RNAseq, small RNAseq, and single cell RNA sequencing.
Proper citation: University of Miami Hussman Institute for Human Genomics Sequencing Core Facility (RRID:SCR_017828) Copy
http://gif.biotech.iastate.edu/
Provides help with grant review, information on data management plans,suggestion best practices for bionformatics analyses, advise on experimental design for Next Gen Sequencing (NGS) projects.Services include:Genome assembly and annotation,Transcriptome assembly and annotation,SNP/InDel calling,RNA-Seq analysis,ChiP-seq,Introgression mapping,novel gene discovery,Personalized GBrowse instances for data visualization,Access to high performance computing, Custom big data projects.
Proper citation: Iowa State University Genome Informatics Core Facility (RRID:SCR_017790) Copy
Core provides next-generation sequencing capabilities using Illumina MiSeq. Helps with experimental design, quality control analysis, library preparation, and data analysis. MiSeq desktop sequencer allows to access applications such as targeted gene sequencing, metagenomics, small genome sequencing, targeted gene expression, amplicon sequencing, and HLA typing.MiSeq is capable of delivering up to 15 Gb of output with 25 million sequencing reads and 2x300 basepair read lengths.
Proper citation: Loyola University Genomics Core Facility (RRID:SCR_017857) Copy
Formerly Center for Genome Research and Biocomputing Core Facility. Functions and facilities include services in genomics, functional genomics, genotyping and imaging.Biocomputing facilities with computing infrastructure, which includes managed cloud and shared resources, data analyses and training are customized to individual needs, including genome assembly and annotation, analysis of RNAseq, GBS, and metagenomics data, and GPU-enabled deep learning analyses.
Proper citation: Oregon State University Center for Quantitative Life Sciences Core Facility (RRID:SCR_018373) Copy
https://github.com/Rinoahu/SwiftOrtho
Software tool for orthology analysis to identify orthologs, paralogs and co orthologs for genomes. Used to perform homology classification across genomes of different species in large genomic datasets.
Proper citation: SwiftOrtho (RRID:SCR_017122) Copy
https://github.com/fenderglass/Flye
Software package as de novo assembler for single molecule sequencing reads. Used for assembling long, error prone reads such as those produced by PacBio and Oxford Nanopore Technologies, for fast and accurate genome reconstructions. Available for Linux and MacOS platforms.
Proper citation: Flye (RRID:SCR_017016) Copy
https://github.com/im3sanger/dndscv
Software R package as suite of dN/dS methods to quantify selection in cancer and somatic evolution. Contains functions to quantify dN/dS ratios for missense, nonsense and essential splice mutations, at level of individual genes, groups of genes or at whole genome level. Used to detect cancer driver genes on datasets.
Proper citation: dndSCV (RRID:SCR_017093) Copy
http://www.cbs.dtu.dk/services/RNAmmer/
Software package to predict ribosomal RNA genes in full genome sequences by utilising two levels of Hidden Markov Models. Consistent and rapid annotation of ribosomal RNA genes.
Proper citation: RNAmmer (RRID:SCR_017075) Copy
Genotype and phenotype database for maize images based on BioDIG. Supports multiple reference genomes and has been integrated with the MaizeGDB Genome Browser to make custom tracks showing mutant phenotypes within their genomic context. Allows for custom tagging of images to highlight regions related to the phenotypes. This is accomplished through an interface allowing users to create links from images to genomic coordinates and to curate and search images by gene model ID, gene symbol, and gene name.
Proper citation: Maize Database of Images and Genomes (RRID:SCR_016987) Copy
https://github.com/ruanjue/wtdbg2.git
Software tool as de novo sequence assembler for long noisy reads produced by PacBio or Oxford Nanopore Technologies. It assembles raw reads without error correction and then builds consensus from intermediate assembly output. Desiged to assemble huge genomes in very limited time.
Proper citation: WTDBG (RRID:SCR_017225) Copy
https://github.com/theaidenlab/3d-dna.git
Software tool as 3D de novo assembly (3D DNA) pipeline. Used to help generate HI-C assembly.
Proper citation: 3D de novo assembly (RRID:SCR_017227) Copy
https://github.com/josephryan/matemaker
Software tool to make artificial mate pairs from long sequences for scaffolding.
Proper citation: matemaker (RRID:SCR_017199) Copy
Software tool for analyzing repetitive DNA found in genome sequences. Software package for identification and classification of genomic repeats. Used for identifying patterns of local alignments induced by certain classes of repeats.
Proper citation: PILER (RRID:SCR_017333) Copy
https://github.com/BDI-pathogens/phyloscanner
Software tool for analysing pathogen genetic diversity and relationships between and within hosts at once, in windows along genome. Inferring transmission from within and between host pathogen genetic diversity.
Proper citation: phyloscanner (RRID:SCR_017400) Copy
Software tool to organize, retrieve, and share genome analysis resources. Reference genome assembly asset manager. In addition to genome indexes, can manage any files related to reference genomes, including sequences and annotation files. Includes command line interface and server application that provides RESTful API, so it is useful for both tool development and analysis.
Proper citation: refgenie (RRID:SCR_017574) Copy
https://github.com/santeripuranen/SpydrPick
Software command line tool for performing direct coupling analysis of aligned categorical datasets. Used for analysis at scale of pan genomes of many bacteria. Incorporates correction for population structure, which adjusts for phylogenetic signal in data without requiring explicit phylogenetic tree.
Proper citation: SpydrPick (RRID:SCR_018176) Copy
https://github.com/santeripuranen/SuperDCA
Software tool for global direct coupling analysis of input genome alignments. Implements variant of pseudolikelihood maximization direct coupling analysis, with emphasis on optimizations that enable its use on genome scale. May be used to discover co evolving pairs of loci.Used for genome wide epistasis analysis.
Proper citation: SuperDCA (RRID:SCR_018175) Copy
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