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Instrument for cell sorting by BD Biosciences where positive air pressure forces sample cell through an optically gel-coupled cuvette . Hydrodynamic focusing guides particles in a single-file stream through the cuvette, where laser light intercepts the stream at the sample interrogation point.Fluorescence activated cell sorting instrument. Flow cytometer as high speed fixed alignment benchtop cell sorter. Enables multicolor analysis of up to 13 fluorescent markers and two scatter parameters at time.
Proper citation: BD Biosciences FACSAria III Cell Sorter (RRID:SCR_016695) Copy
https://www.leicabiosystems.com/us/histology-equipment/cryostats/leica-cm3050-s/
Instrument designed for cryosectioning in biomedical, neuro-anatomical and pharmaceutical research.
Proper citation: Leica CM3050 S Research Cryostat (RRID:SCR_016844) Copy
https://www.thermofisher.com/order/catalog/product/3730XL#/3730XL
96-capillary DNA analyzer that performs DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Has multiple automation features. Performs sequencing and fragment analysis applications including resequencing, microsatellite analysis, AFLP, LOH, SSCP, SNP screening and SNP validation.
Proper citation: Applied Biosystems 3730xl Capillary Genetic Sequencer (RRID:SCR_018059) Copy
Femto Pulse System separates large molecular weight DNA fragments and detects nucleic acids into femtogram range.Fragment analyzer that provides pulsed field power supply which allows large molecular weight DNA fragments to be separated and nucleic acids detected into femtogram range. Ability to separate high molecular weight DNA up to 165 kb and detect nucleic acids down to 50 fg per uL input concentration makes system ideal for long-read NGS QC, gDNA, small RNA, or cfDNA analysis from low concentration samples.
Proper citation: Agilent Femto Pulse System (RRID:SCR_018058) Copy
https://www.thermofisher.com/order/catalog/product/3730S#/3730S
Medium to high throughput genetic analyzer that performs DNA fragment analysis applications such as microsatellites, AFLP, SNP analysis, mutation detection and traditional DNA sequencing. Upgradeable to 96 capillaries.
Proper citation: Applied Biosystems 3730 Genetic Analyzer (RRID:SCR_018052) Copy
Refridgerated centrifuge with 18 place capacity rotor that accomdates low to medium capacity in 1.5 or 2.0 mL tubes and Microtainer. Speed allows for standard molecular biology applications. Compact size and quiet operation.
Proper citation: Eppendorf 5418 R Centrifuge (RRID:SCR_018050) Copy
https://www.bio-rad.com/en-us/product/cfx384-touch-real-time-pcr-detection-system?ID=LJB22YE8Z
Real Time PCR instrument in 384-well format that performs high-throughput data acquisition. System utilizes temperature control and sensitive, 4-color detection optics to deliver qPCR results for singleplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on integrated LCD touch screen, or use CFX Maestro Software to design experiment and analyze results from connected computer.
Proper citation: Bio-Rad CFX384 Real-Time Detection System (RRID:SCR_018057) Copy
Flow cytometry system that performs reliable performance, accuracy, and ease-of-use for clinical diagnostic services. System has 10 color capability, optical enhancements, and 4-3-3 configuration deliver high sensitivity and resolution for accurate results.
Proper citation: BD Scientific FACSCanto Flow Cytometer (RRID:SCR_018055) Copy
MRI system that performs preclinical and molecular MR imaging. MRI CryoProbe technology combined with ultra high field USR magnets deliver high spatial resolution in-vivo. Any small animal MR imaging application in life science, biomedical and preclinical research can be conducted.
Proper citation: Bruker 9.4T Biospec Biospec Magnetic Resonance Imaging (RRID:SCR_018054) Copy
https://www.bio-rad.com/en-us/product/cfx96-touch-real-time-pcr-detection-system?ID=LJB1YU15
Real Time PCR detection system that combines optical technology with temperature control. Six channel, five colors and one FRET channel, real-time PCR instrument for singlexplex or multiplex reactions. Quickly set up runs and monitor amplification traces in real time on integrated LCD touch screen, or use included CFX Maestro Software to design experiment and analyze results from connected computer.
Proper citation: Bio-Rad CFX96 Real-Time PCR Detection System (RRID:SCR_018064) Copy
https://www.selectscience.net/products/zeiss-lsm-710-for-fluorescence-imaging/?prodID=84549#tab-2
Inverted microscope that enables confocal microscopy in cell and developmental biology.
Proper citation: Zeiss LSM 710 Confocal Inverted Microscope (RRID:SCR_018063) Copy
https://www.biocompare.com/Product-Reviews/41153-LSM-510-META-From-Zeiss/
Versatile laser scanning confocal microscope system that is available in both upright or inverted format. Equipped with ICS-Optics. Selection of dry, water immersion and oil immersion objectives are available. ICS-Optics are infinity color corrected.
Proper citation: Zeiss LSM 510 Confocal Microscope (RRID:SCR_018062) Copy
https://www.sonybiotechnology.com/us/instruments/sh800s-cell-sorter/
Benchtop cell sorter that sorts wide range of cell sizes for many applications using microfluidic sorting chips. Integrated with comprehensive fluidics controls and advanced automation for set-up, acquisition, sort and analysis. System software supports sorting into tubes and 96- and 384-well plates. Software generates FCS 3.0 and FCS 3.1 files that can be exported to third party analysis tools. For non-clinical research use only. For applications in which cross-contamination is concern, optional e-beam sterilized consumables can be used to replace sample line and sorting chip. Optional class A2 Level II biosafety cabinet provides protection for personnel and products. Biosafety cabinet meets international standards.Optical design offers up to four collinear excitation lasers (488 nm, 405nm, 561nm and 638nm) and six fluorescence detectors. The six free-form PMTs enable detection of fluorescence signals from any laser based on filter selection.
Proper citation: Sony Biotechnology SH800S Cell Sorter (RRID:SCR_018066) Copy
Paraffin embedding station that provides constant, reproducible paraffin flow at ten different flow rate settings. All instrument functions, including automatic starting time, are individually programmable at press of button. Compact dimensions, ease of operation, and high standards of convenience. Separately-heated paraffin dispensing system with integrated filter provides constant, reproducible paraffin flow at ten different flow rate settings.
Proper citation: Leica EG1160 Embedding Station (RRID:SCR_018033) Copy
https://www.labx.com/item/molecular-devices-molecular-dynamics-storm-860-molecular/3896212
Multifunction phosphoimager fluorimager laser scanner that collects fluorescent or storage phosphor images from gels, membranes and phosphor screens. It is equipped with blue (450 nm) and red (635 nm) lasers, can scan at resolutions down to 50 microns. It is able to quantify radioactive gels for commonly used isotopes such as 32P, 33P, 125I, 35S, and 14C. There are three detection modes: phosphor screen mode, red/blue fluorescence, chemifluorescence.
Proper citation: Molecular Dynamics Storm 860 Molecular Imager (RRID:SCR_018032) Copy
https://www.thermofisher.com/order/catalog/product/4315931#/4315931
Genetic analyzer that performs fragment analysis and rapid sequencing applications. Usage per array is 100 runs with use of Applied Biosystems reagents.
Proper citation: Applied Biosystems 3130xl Capillary Array (RRID:SCR_018048) Copy
https://bionano.com/saphyr-systems/
Whole genome imaging instrument by Bionano Genomics for high speed, high throughput structural variant detection and analysis with exceptional sensitivity and specificity. Detects structural variations ranging from 500 bp to megabase pairs in length. For mosaic samples or heterogeneous cancer samples, detects all types of structural variants down to 5% Variant Allele Fraction. Calls repeats and complex rearrangements.
Proper citation: Saphyr (RRID:SCR_017992) Copy
https://www.thelabworldgroup.com/ge-typhoon-9410-microarray-imager
Variable mode imager that operates in three different modes, including phosphorescence, fluorescence, and chemiluminescence. Can scan in visible range using reflected light. Scanning resolution ranges from 1000um down to 10um. Scanning software is linked to ImageQuant TL 7.0 analysis software. ImageQuant is capable of quantifying Typhoon images such as those from Northern or Western blots. Images can be saved in various formats and imported into Powerpoint slides. Instrument has three excitation lasers (532nm, 633nm, 488nm). Instrument utilizes 536SP, 670BP, 520BP emission filters for fluorescence. For DNA, RNA and protein samples you may choose from: storage phosphor auto-radiography, direct blue-excited fluorescence (457-488 nm), direct green-excited fluorescence (532 nm), direct red-excited fluorescence (633 nm), and chemiluminescence.
Proper citation: GE Healthcare 9410 Variable Mode Imager (RRID:SCR_018047) Copy
https://www.leicabiosystems.com/histology-equipment/microtomes/products/leica-rm2265/
Automated rotary microtome that sections tissue samples. Specimen feed system with zero-backlash and maintenance-free cross roller guides and stepper motor operated coarse feed system are located in dust-proof plastic housing. Equipped with safety handwheel with handle that can be centered, as well as mechanical handwheel lock. In manual operation retraction can be adjusted. In motorized operation retraction value varies with selected sectioning speed.
Proper citation: Leica Biosystems RM2265 Fully Automated Rotary Microtome (RRID:SCR_018041) Copy
https://www.leicabiosystems.com/histology-equipment/microtomes/leica-rm2125-rts/
Manual rotary microtome that sections tissue samples. Features include precise feed mechanism, powerBase that ensures stable operation for vibration-free sectioning, advanced knife holder and specimen clamp securely align cutting edge to block, user-selectable coarse feed wheel turn direction and retraction functions, hand wheel, in-built safety guards and rounded shape with arm rests for fatigue-free sectioning,quick-exchange specimen clamp and orientation system for object head allow rapid set up for any block, and two trim steps.
Proper citation: Leica RM2125 RTS Manual Microtome (RRID:SCR_018040) Copy
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