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SciCrunch Registry is a curated repository of scientific resources, with a focus on biomedical resources, including tools, databases, and core facilities - visit SciCrunch to register your resource.
https://www.kentscientific.com/products/somnoflo/
Compact, standalone vaporizer that uses either ambient air with internal air pump or compressed gas. It delivers only anesthetic required by animal according to its weight with flows down to 0.05 LPM. Low flow isoflurane anesthesia machine.
Proper citation: SomnoFlow Low Flow Electronic Vaporizer (RRID:SCR_021629) Copy
https://www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-dsp-overview/
Integrated commercial system comprising hardware, software and nCounter chemistry that enables simultaneous, highly multiplex spatial profiling of proteins or RNA in FFPE tissues. DSP platform quantifies abundance of protein or RNA by counting unique indexing oligonucleotides assigned to each target of interest. Used to rapidly and quantitatively assess biological implications of heterogeneity within tissue samples.
Proper citation: NanoString GeoMx Digital Spatial Profiler (RRID:SCR_021660) Copy
https://meyerinst.com/library/NanoZoomer_RS_Brochure_Catalog.pdf
High speed, high resolution digital scanner with network features. User friendly full slide scanner designed to process up to 210 slides automatically.
Proper citation: NanoZoomer 2.0-HT (RRID:SCR_021658) Copy
System includes automated cell counter for trypan blue viability for cell lines and bright field imaging and pattern recognition software to quickly and accurately identify and count individual cells. Cell count, concentration, diameter, and % viability are automatically calculated and reported.
Proper citation: Cellometer Auto T4 (RRID:SCR_021656) Copy
https://www.nanostring.com/products/ncounter-analysis-system/ncounter-systems-overview/
System includes hardware and software. Options include nCounter SPRINT,nCounter MAX or nCounter FLEX. Platform used for multiplex analysis of up to 800 RNA, DNA, or protein targets. System can be combined with GeoMx Digital Spatial Profiler (DSP) to enable high-plex, spatially-resolved RNA, and protein quantification.
Proper citation: NanoString nCounter Analysis System (RRID:SCR_021712) Copy
https://www.cellscale.com/products/microtester/
System includes MicroTester G2 device and software. MicroTester G2 is mechanical test device that can be used to test properties of variety of tissues and materials. Main components of device are load application microbeam, actuators, imaging system, and fluid bath/test chamber. MicroTester G2 software is divided into two modules: data collection module and review and analysis module.
Proper citation: MicroTester G2 system (RRID:SCR_022034) Copy
https://www.coolled.com/products/pe-300ultra/
Highly controllable fluorescence microscopy Illumination System which offers broad spectrum LED illumination for imaging most common fluorophores. System offers precise control over wavelength irradiance and shuttering. Used for LED microscope lighting.
Proper citation: CoolLED pE-300ultra (RRID:SCR_021972) Copy
https://www.alveolelab.com/our-products/primo-micropatterning/
Photopatterning device for performing micropatterning, microfabrication and hydrogel polymerization.Part of PRIMO system.
Proper citation: Alveole PRIMO photopatterning module (RRID:SCR_021941) Copy
https://biomedical-instruments.com/pdf/Narishige%20PC-10.pdf
Vertical pipette puller to create microelectrodes from borosilicate capillaries.
Proper citation: Narishige PC 10 Puller (RRID:SCR_022057) Copy
Horizontal pipette puller to pull and polish microelectrodes from borosilicate capillaries.
Proper citation: DMZ Universal Electrode Puller (RRID:SCR_022056) Copy
https://biomedical-instruments.com/pdf/Narishige%20MF-830.pdf
Powerful microscope mounted to produce microelectrodes for patch clamping.
Proper citation: Narishige MF 830 Microgrinder (RRID:SCR_022055) Copy
https://www.bio-rad.com/en-us/product/t100-thermal-cycler?ID=LGTWGIE8Z
Small compact 96well thermal cycler. PCR system features intuitive touch screen and thermal gradient technology to optimize reaction in single run.
Proper citation: BioRad T100 Thermal Cycler (RRID:SCR_021921) Copy
https://www.visualsonics.com/product/imaging-systems/vevo-3100
Vevo 3100 micro ultrasound imaging system reduces speckle noise and artifacts while preserving and enhancing critical information for small animal in vivo studies. System helps you visualize your data at high resolution.
Proper citation: Vevo 3100 Imaging System (RRID:SCR_022152) Copy
Designed to eliminate complexities of microscopy, EVOS XL Core system captures high-quality brightfield cell images right at your benchtop within minute. Instrument for monitoring cell cultures, either within hood or in cell culture room.
Proper citation: EVOS XL Core Imaging System (RRID:SCR_022190) Copy
https://jwst.nasa.gov/content/observatory/instruments/nircam.html
Instrument aboard the James Webb Space Telescope. It has two major tasks, as imager from 0.6 to 5 micron wavelength, and as wavefront sensor to keep 18-section mirrors functioning as one. Imager covers infrared wavelength range 0.6 to 5 microns. NIRCam detects light from the earliest stars and galaxies in process of formation, population of stars in nearby galaxies, as well as young stars in Milky Way and Kuiper Belt objects. NIRCam is equipped with coronagraphs, instruments that allow astronomers to take pictures of very faint objects around central bright object, like stellar systems.
Proper citation: Near Infrared Camera (RRID:SCR_022113) Copy
https://mass-spec.stanford.edu/instruments
System includes Thermo Orbitrap Eclipse Tribid mass spectrometer and Waters M-Class Acquity nanoUPLC. This platform features sequential acquisition capabilities and multiple fragmentation types, enabling complex, in depth proteomic experiments.
Proper citation: Thermo Orbitrap Eclipse nanoLC/MS system (RRID:SCR_022212) Copy
https://www.olympus-lifescience.com/data/olympusmicro/brochures/pdfs/ix71.pdf?rev=EABE
Research inverted system microscope. Olympus IX2 inverted microscope combined with UIS2 optical system. Used for live cell experiments.
Proper citation: Olympus IX71 Microscope (RRID:SCR_022185) Copy
http://edboyden.org/05.09.boyden.html
Laser tool that enables neurons to be optically silenced by pulses of yellow light, the light-activated chloride pump halorhodopsin (Halo), in a paper entitled Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers. The quest to determine how precise neural activity patterns mediate computation, behavior, and pathology would be greatly aided by a set of tools for reliably activating and inactivating genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having earlier adapted a light-activated cation channel, 1channelrhodopsin-2 (ChR2), for allowing neurons to be stimulated by blue light, we searched for a complementary tool that would enable optical neuronal inhibition, driven by light of a second color. Here we report that targeting the 1codon-optimized form of the light-driven chloride pump halorhodopsin from the archaebacterium Natronomas pharaonis (hereafter abbreviated Halo) to genetically-specified neurons enables them to be silenced reliably, and reversibly, by millisecond-timescale pulses of yellow light. We show that trains of yellow and blue light pulses can drive high-fidelity sequences of hyperpolarizations and depolarizations in neurons simultaneously expressing yellow light-driven Halo and blue light-driven ChR2, allowing for the first time manipulations of neural synchrony without perturbation of other parameters such as spiking rates. The Halo/ChR2 system thus constitutes a powerful toolbox for multichannel photoinhibition and photostimulation of virally or transgenically targeted neural circuits without need for exogenous chemicals, enabling systematic analysis and engineering of the brain, and quantitative bioengineering of excitable cells.
Proper citation: Channelrhodopsin-2 enables optical activation of neurons (RRID:SCR_008833) Copy
Benchtop genetic analyzer. Electrophoresis system for DNA and RNA sample quality control. It can be used for next generation sequencing or Biobank workflow.
Proper citation: Agilent 4200 TapeStation System (RRID:SCR_018435) Copy
http://tools.thermofisher.com/content/sfs/manuals/cms_040970.pdf
Automated PCR instrument for automated amplification of nucleic acids with Polymerase Chain Reaction. It has reaction volumes of up to 50 uL and sample temperature range of 4 to 99.9 C.
Proper citation: Applied Biosystems GeneAmp 9700 PCR Thermocycler System (RRID:SCR_018436) Copy
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