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http://actimetrics.com/products/limelight/

A video tracking system designed for high-throughput behavioral experiments. LimeLight can track up to 4 arenas at once and can collect images at up to 30 frames per second for one animal or up to 8 frames per second when tracking 4 arenas at once. The tracking system has 2 different hardware configurations: the 1-camera system can be used to record 1 to 4 animals at once, while for multiple animals, each one is placed in a separate arena in one quadrant of the image. The software contains productivity features such as flexible arena setup, user-defined behavior scoring, stimulus control, and various analytical functions for quantifying behavior. The program is designed for experiments such as Open Field, Plus Maze, Radial Arm Maze, Zero Maze, Novel Object Recognition, Conditioned Place Preference, and Barnes Maze.

Proper citation: Limelight (RRID:SCR_014254) Copy   

•   Source: SciCrunch Registry

https://www.bio-rad.com/en-us/product/ze5-cell-analyzer?ID=OC62Q015

BioRad ZE5 was formerly known as the Propel Labs YETI Cytometer with configurations to meet broad range of experimental complexities and throughput needs. Accessible for novice flow cytometry users yet flexible enough for most experienced flow cytometry professionals.

Proper citation: Bio-Rad ZE5 Cell Analyzer (RRID:SCR_019712) Copy   

•   Source: SciCrunch Registry

https://edspace.american.edu/openbehavior/project/automated-mouse-homecage-two-bottle-choice-test/

Project related to assessing preferences by mice among fluids in their homecages.Provided system includes homecage fitted apparatus for automated, photobeam based detection of licks in two bottle choice task. Used for automated mouse homecage two bottle choice test.

Proper citation: Automated mouse homecage two bottle choice test project (RRID:SCR_021457) Copy   

•   Source: SciCrunch Registry

https://edspace.american.edu/openbehavior/project/rad/

Project related to tracking and recording activity in rodent home cages.Provides device that utilizes passive infrared (PIR) sensor to detect movement in cages and logs data to microSD card. Device is wireless and battery powered, and can record data for about 3 weeks on charge.

Proper citation: Rodent Activity Detector project (RRID:SCR_021451) Copy   

•   Source: SciCrunch Registry

https://edspace.american.edu/openbehavior/project/homecage-2bottle-choice/

Project related to studies of liquid ingestive behaviors used in neuroscience to investigate reward related behavior, metabolism, and circadian biology. Provides automated home cage sipper device for monitoring liquid ingestive behavior in rodents developed by Washington University, St. Louis scientists. Homecage fitted apparatus for automated, photobeam based detection of licks in two bottle choice task.

Proper citation: Automated Home Cage Rodent Two bottle Choice Test project (RRID:SCR_021445) Copy   

•   Source: SciCrunch Registry

https://timothyspringer.org/files/tas/files/biacore3000-instrument.pdf

Biacore 3000 processing unit is an established, label-free system for detailed studies of biomolecular interactions. The system delivers comprehensive characterization of the interaction, answers questions about the rate constants, affinity, specificity, and determines the active concentration of components. The ability to recover and transfer interaction partners directly to MALDI targets for identification and further characterization makes the system highly applicable to fast identification of unknown interactants.

Proper citation: Biacore 3000 Real Time Biomolecular Interaction Analyzer (RRID:SCR_018044) Copy   

•   Source: SciCrunch Registry

https://www.microscopyu.com/museum/model-smz1500-stereomicroscope

SMZ1500 stereoscopic zoom microscope is system with apochromatic objectives, zoom ratio of 15 to 1, range of magnifications from 0.75x to 11.25x for total magnifying capability of 3.75x up to 540x, and is best for in depth imaging.Zoom knob allows to zoom from 1x to 11x at 1x-intervals without interrupting observations. Other facets include high eyepoint eyepieces with built-in diopter adjustments, wide field of view, and built-in aperture diaphragm for achieving optimal depth of focus and improved photomicrography.Provides options of either low eye-level viewing tube or tilting eyepiece tube, with or without eye-level risers. Three different diascopic stands with built-in power supplies may also be utilized with instrument, or standard stand that features low profile for increased clearance.

Proper citation: Nikon SMZ1500 Zoom Stereoscope (RRID:SCR_018605) Copy   

•   Source: SciCrunch Registry

http://edboyden.org/05.09.boyden.html

Laser tool that enables neurons to be optically silenced by pulses of yellow light, the light-activated chloride pump halorhodopsin (Halo), in a paper entitled Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers. The quest to determine how precise neural activity patterns mediate computation, behavior, and pathology would be greatly aided by a set of tools for reliably activating and inactivating genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having earlier adapted a light-activated cation channel, 1channelrhodopsin-2 (ChR2), for allowing neurons to be stimulated by blue light, we searched for a complementary tool that would enable optical neuronal inhibition, driven by light of a second color. Here we report that targeting the 1codon-optimized form of the light-driven chloride pump halorhodopsin from the archaebacterium Natronomas pharaonis (hereafter abbreviated Halo) to genetically-specified neurons enables them to be silenced reliably, and reversibly, by millisecond-timescale pulses of yellow light. We show that trains of yellow and blue light pulses can drive high-fidelity sequences of hyperpolarizations and depolarizations in neurons simultaneously expressing yellow light-driven Halo and blue light-driven ChR2, allowing for the first time manipulations of neural synchrony without perturbation of other parameters such as spiking rates. The Halo/ChR2 system thus constitutes a powerful toolbox for multichannel photoinhibition and photostimulation of virally or transgenically targeted neural circuits without need for exogenous chemicals, enabling systematic analysis and engineering of the brain, and quantitative bioengineering of excitable cells.

Proper citation: Channelrhodopsin-2 enables optical activation of neurons (RRID:SCR_008833) Copy   

•   Source: SciCrunch Registry

http://www.med.upenn.edu/molecular/core_morphology.shtml

Core facility that provides histological services, equipment usage, and technical expertise to digestive and liver research projects.

Proper citation: University of Pennsylvania Center for Molecular Studies in Digestive and Liver Diseases Molecular Pathology and Imaging Core (RRID:SCR_015618) Copy   

•   Source: SciCrunch Registry

http://tools.thermofisher.com/content/sfs/manuals/cms_040970.pdf

Automated PCR instrument for automated amplification of nucleic acids with Polymerase Chain Reaction. It has reaction volumes of up to 50 uL and sample temperature range of 4 to 99.9 C.

Proper citation: Applied Biosystems GeneAmp 9700 PCR Thermocycler System (RRID:SCR_018436) Copy   

•   Source: SciCrunch Registry

https://dna.uga.edu/

Core laboratory for nucleic acid sequencing and bioinformatics. Used for research support, education, and training. Services include genomic techniques and applications, sequencing technologies, and bioinformatics analyses, writting letters of support for grant applications submitted to funding agencies. GGBC operates multiple platforms for short-, long-, and single-molecule sequencing reads (i.e., Illumina MiSeq and NextSeq, PacBio Sequel, and Oxford Nanopore MinIon).

Proper citation: Georgia Genomics and Bioinformatics Core at the University of Georgia (RRID:SCR_010994) Copy   

•   Source: SciCrunch Registry

http://www.rockefeller.edu/bioimaging/

Provide members of University and their visitors with wide spectrum of optical microscopy equipment and extensive training in its use.

Proper citation: Rockefeller University Bio-Imaging Resource Center Core Facility (RRID:SCR_017791) Copy   

•   Source: SciCrunch Registry

https://biophy.uchicago.edu/

Core provides access to biophysics equipment, integral to research community at University of Chicago.Provides tools, training, and assistance for quantitative analysis of macromolecules and their interactions. Instruments include Seahorse XFe-96 Extracellular Flux Analyzer,Jasco J-1500 CD Spectrometer,Synergy Neo HST Plate Reader,Wyatt DAWN HELEOS II SLS,Wyatt DynaPro Plate Reader DLS,Wyatt DynaPro NanoStar DLS,HORIBA Fluorolog-3,Microcal iTC200,Molecular Imager,Bio-Rad ProteOn XPR to Biacore 8K,Agilent 8453 Spectrophotometer. Core provides staff assistance in conducting experiments.

Proper citation: Chicago University Biophysics Core Facility (RRID:SCR_017915) Copy   

•   Source: SciCrunch Registry

https://www.brown.edu/research/projects/superfund/cores/core-d

Core provides equipment and technical expertise for evaluation of molecular and morphological changes in cells, tissues, and organs following exposure to complex environmental contaminants. Provides equipment, including automated tissue processor, paraffin embedding center, two automated microtomes, cryostat, vibratome for soft-tissue sectioning, multiheaded light microscope with projection capabilities, system for laser capture microdissection, and slide scanner with analysis software for identification and quantification of morphological structures.Offers expertise in histopathological and immunocytochemial methods, including fixation, dehydration, embedding, sectioning, histological staining, immunolabeling, high-resolution imaging, and quantitative image analysis. Services in sample preparation, offers assistance in imaging and image analysis and provides consultation for ongoing or future research projects, training program for students and investigators who use centrally available equipment.

Proper citation: Brown University Molecular Pathology Core Facility (RRID:SCR_017898) Copy   

•   Source: SciCrunch Registry

https://www.igb.illinois.edu/corefacilities

Core for biological microscopy and image analysis. Offers high-end equipment,user training,ongoing support, including experiment design and data interpretation,twenty-four hour access Services,3D Printing, Transfer Files, Image Analysis,Histology,Instrument Training.

Proper citation: University of Illinois at Urbana-Champaign Core Facilities at IGB (RRID:SCR_017938) Copy   

•   Source: SciCrunch Registry

https://research.unimelb.edu.au/facilities-and-resources/research-infrastructure/biological-optical-microscopy-platform

Core provides access to fluorescence microscopy equipment including Deltavision OMX Blaze Super Resolution (3D-SIM/SMLM), Leica SP8 inverted confocal, La Vision Ultramicroscope II Lightsheet Microscope,Nikon Andor WD spinning disk, Nikon A1R inverted confocal x2, Olympus FV-MPERS Multi Photon,Olympus FV3000 inverted confocal (live cell) ISS Fast-FLIM, Perkin Elmer Operetta High Content Imaging, Zeiss LSM800 upright Airyscan, Zeiss LSM880 upright Airyscan fast, Zeiss Elyra/LSM880 inverted (live cell), super resolution (Airyscan/SMLM). Staff can help with image analysis, super resolution microscopy, fluorescence lifetime imaging microscopy, high content imaging and light sheet microscopy techniques.

Proper citation: University of Melbourne Biological Optical Microscopy Platform (BOMP) Core Facility (RRID:SCR_018888) Copy   

•   Source: SciCrunch Registry

https://www.sutter.com/manuals/P-2000_OpMan.pdf

Microprocessor controlled, CO2 laser based micropipette puller.Default configuration allows fabrication of micropipettes for intracellular recording, patch clamping, microinjection and microperfusion.Can store up to 100 separate programs, each consisting of up to 8 command lines. The five parameters set in each command line allow exquisite control of the micropipette taper geometry. Allows the use of wide range of glass diameters from 0.125 to 1.2 mm.

Proper citation: Sutter P-2000 Laser Micropipette Puller (RRID:SCR_018640) Copy   

•   Source: SciCrunch Registry

https://site.research.utexas.edu/cbrs/

Facility provides access to and operational support for easy-to-operate equipment for sample preparation and analysis, including gel imagers, plate readers, and qPCRs. They provide training on instrument operation and grant access for independent use after completed training.

Proper citation: University of Texas at Austin Shared Instrumentation Facility (RRID:SCR_026271) Copy   

•   Source: SciCrunch Registry

http://www.stanford.edu/group/brainsinsilicon/neurogrid.html

A specialized hardware platform that will perform cortex-scale emulations while offering software-like flexibility. With sixteen 12x14 sq-mm chips (Neurocores) assembled on a 6.5x7.5 sq-in circuit board that can model a slab of cortex with up to 16x256x256 neurons - over a million! The chips are interconnected in a binary tree by 80M spike/sec links. An on-chip RAM (in each Neurocore) and an off-chip RAM (on a daughterboard, not shown) softwire vertical and horizontcal cortical connections, respectively. It provides an affordable option for brain simulations that uses analog computation to emulate ion-channel activity and uses digital communication to softwire synaptic connections. These technologies impose different constraints, because they operate in parallel and in serial, respectively. Analog computation constrains the number of distinct ion-channel populations that can be simulatedunlike digital computation, which simply takes longer to run bigger simulations. Digital communication constrains the number of synaptic connections that can be activated per secondunlike analog communication, which simply sums additional inputs onto the same wire. Working within these constraints, Neurogrid achieves its goal of simulating multiple cortical areas in real-time by making judicious choices.

Proper citation: Neurogrid (RRID:SCR_005024) Copy   

•   Source: SciCrunch Registry

http://www.sartorius.com/

Commercial organization that provides laboratory and process technologies and equipment. Their products and services help customers around the globe implement complex and quality-critical processes in biopharmaceutical production and laboratory environments in a time- and cost-efficient way. Sartorius operates its own production facilities in Europe, Asia and America, and also has sales offices and local representatives in more than 110 countries.

Proper citation: Sartorius (RRID:SCR_003935) Copy   

•   Source: SciCrunch Registry