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http://edboyden.org/05.09.boyden.html

Laser tool that enables neurons to be optically silenced by pulses of yellow light, the light-activated chloride pump halorhodopsin (Halo), in a paper entitled Multiple-color optical activation, silencing, and desynchronization of neural activity, with single-spike temporal resolution. Temporally precise, noninvasive control of activity in well-defined neuronal populations is a long-sought goal of systems neuroscience. We adapted for this purpose the naturally occurring algal protein Channelrhodopsin-2, a rapidly gated light-sensitive cation channel, by using lentiviral gene delivery in combination with high-speed optical switching to photostimulate mammalian neurons. We demonstrate reliable, millisecond-timescale control of neuronal spiking, as well as control of excitatory and inhibitory synaptic transmission. This technology allows the use of light to alter neural processing at the level of single spikes and synaptic events, yielding a widely applicable tool for neuroscientists and biomedical engineers. The quest to determine how precise neural activity patterns mediate computation, behavior, and pathology would be greatly aided by a set of tools for reliably activating and inactivating genetically targeted neurons, in a temporally precise and rapidly reversible fashion. Having earlier adapted a light-activated cation channel, 1channelrhodopsin-2 (ChR2), for allowing neurons to be stimulated by blue light, we searched for a complementary tool that would enable optical neuronal inhibition, driven by light of a second color. Here we report that targeting the 1codon-optimized form of the light-driven chloride pump halorhodopsin from the archaebacterium Natronomas pharaonis (hereafter abbreviated Halo) to genetically-specified neurons enables them to be silenced reliably, and reversibly, by millisecond-timescale pulses of yellow light. We show that trains of yellow and blue light pulses can drive high-fidelity sequences of hyperpolarizations and depolarizations in neurons simultaneously expressing yellow light-driven Halo and blue light-driven ChR2, allowing for the first time manipulations of neural synchrony without perturbation of other parameters such as spiking rates. The Halo/ChR2 system thus constitutes a powerful toolbox for multichannel photoinhibition and photostimulation of virally or transgenically targeted neural circuits without need for exogenous chemicals, enabling systematic analysis and engineering of the brain, and quantitative bioengineering of excitable cells.

Proper citation: Channelrhodopsin-2 enables optical activation of neurons (RRID:SCR_008833) Copy   

•   Source: SciCrunch Registry

https://icahn.mssm.edu/research/human-immune-monitoring-center

Core provides team of experts in the characterization of immune profiles and treatment responses across a diverse range of diseases, such as cancer, autoimmunity, allergy, and neuro-immune disorders. Offers equipment in the field for research at Genomic, cellular and proteomic analysis. Develops assays to balance innovation with protocols and operating procedures to ensure data quality and reproducibility.

Proper citation: Icahn School of Medicine at Mount Sinai Human Immune Monitoring Center Core Facility (RRID:SCR_027571) Copy   

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https://site.research.utexas.edu/cbrs/

Facility provides access to and operational support for easy-to-operate equipment for sample preparation and analysis, including gel imagers, plate readers, and qPCRs. They provide training on instrument operation and grant access for independent use after completed training.

Proper citation: University of Texas at Austin Shared Instrumentation Facility (RRID:SCR_026271) Copy   

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https://biophy.uchicago.edu/

Core provides access to biophysics equipment, integral to research community at University of Chicago.Provides tools, training, and assistance for quantitative analysis of macromolecules and their interactions. Instruments include Seahorse XFe-96 Extracellular Flux Analyzer,Jasco J-1500 CD Spectrometer,Synergy Neo HST Plate Reader,Wyatt DAWN HELEOS II SLS,Wyatt DynaPro Plate Reader DLS,Wyatt DynaPro NanoStar DLS,HORIBA Fluorolog-3,Microcal iTC200,Molecular Imager,Bio-Rad ProteOn XPR to Biacore 8K,Agilent 8453 Spectrophotometer. Core provides staff assistance in conducting experiments.

Proper citation: Chicago University Biophysics Core Facility (RRID:SCR_017915) Copy   

•   Source: SciCrunch Registry

https://www.brown.edu/research/projects/superfund/cores/core-d

Core provides equipment and technical expertise for evaluation of molecular and morphological changes in cells, tissues, and organs following exposure to complex environmental contaminants. Provides equipment, including automated tissue processor, paraffin embedding center, two automated microtomes, cryostat, vibratome for soft-tissue sectioning, multiheaded light microscope with projection capabilities, system for laser capture microdissection, and slide scanner with analysis software for identification and quantification of morphological structures.Offers expertise in histopathological and immunocytochemial methods, including fixation, dehydration, embedding, sectioning, histological staining, immunolabeling, high-resolution imaging, and quantitative image analysis. Services in sample preparation, offers assistance in imaging and image analysis and provides consultation for ongoing or future research projects, training program for students and investigators who use centrally available equipment.

Proper citation: Brown University Molecular Pathology Core Facility (RRID:SCR_017898) Copy   

•   Source: SciCrunch Registry

https://www.igb.illinois.edu/corefacilities

Core for biological microscopy and image analysis. Offers high-end equipment,user training,ongoing support, including experiment design and data interpretation,twenty-four hour access Services,3D Printing, Transfer Files, Image Analysis,Histology,Instrument Training.

Proper citation: University of Illinois at Urbana-Champaign Core Facilities at IGB (RRID:SCR_017938) Copy   

•   Source: SciCrunch Registry

https://biomedicalsciences.unimelb.edu.au/departments/department-of-biochemistry-and-pharmacology/research/services/bomp

Core provides access to fluorescence microscopy equipment including Deltavision OMX Blaze Super Resolution (3D-SIM/SMLM), Leica SP8 inverted confocal, La Vision Ultramicroscope II Lightsheet Microscope,Nikon Andor WD spinning disk, Nikon A1R inverted confocal x2, Olympus FV-MPERS Multi Photon,Olympus FV3000 inverted confocal (live cell) ISS Fast-FLIM, Perkin Elmer Operetta High Content Imaging, Zeiss LSM800 upright Airyscan, Zeiss LSM880 upright Airyscan fast, Zeiss Elyra/LSM880 inverted (live cell), super resolution (Airyscan/SMLM). Staff can help with image analysis, super resolution microscopy, fluorescence lifetime imaging microscopy, high content imaging and light sheet microscopy techniques.

Proper citation: University of Melbourne Biological Optical Microscopy Platform (BOMP) Core Facility (RRID:SCR_018888) Copy   

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http://www.pstnet.com/hardware.cfm?ID=92

MRI Simulator that provides a realistic approximation of an actual MRI scanner to allow habituation and training of participants in an environment less daunting than a real scanner. Special populations such as children, the elderly, and psychiatric patients, are often prone to claustrophobia and anxiety in the bore of a magnet, and consequently have a much higher rate of terminating the experiment or scan session before its completion. Some centers that have dealt with these populations estimate a 50%-80% failure rate. With the use of the MRI Simulator this failure rate can often be reduced below 5%, improving cost effectiveness.

Proper citation: PSTNet: MRI Simulator (RRID:SCR_002460) Copy   

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http://www.pstnet.com/hardware.cfm?ID=90

MRI Digital Projection System that uses Digital Light Processing (DLP) technology providing microsecond pixel rise times, outstanding contrast with all-digital fiber optic control that allows you to project crystal clear, sharp images. Includes: * High resolution (1024x768) DLP Projector with RF filtered enclosure, custom lens assembly, digital video (DVI) over fiber, high flow fans, internal thermal sensor * Control room device to perform DVI to Fiber conversion, remotely power down the projector, and allow use of projector remote control from control room * 30 meter fiber optic cable that runs between the projector and projector control station * Heavy duty, magnet compatible, projector stand (assembly required) * Heavy duty, magnet compatible mirror stand with mirror (assembly required) * High resolution, lenticular pitch rear projection screen for high quality image reproduction * Optional VGA to DVI converter (native DVI video cards on Windows or Macintosh recommended)

Proper citation: MRI Digital Projection System (RRID:SCR_002486) Copy   

•   Source: SciCrunch Registry

http://www.sartorius.com/

Commercial organization that provides laboratory and process technologies and equipment. Their products and services help customers around the globe implement complex and quality-critical processes in biopharmaceutical production and laboratory environments in a time- and cost-efficient way. Sartorius operates its own production facilities in Europe, Asia and America, and also has sales offices and local representatives in more than 110 countries.

Proper citation: Sartorius (RRID:SCR_003935) Copy   

•   Source: SciCrunch Registry

http://www.stanford.edu/group/brainsinsilicon/neurogrid.html

A specialized hardware platform that will perform cortex-scale emulations while offering software-like flexibility. With sixteen 12x14 sq-mm chips (Neurocores) assembled on a 6.5x7.5 sq-in circuit board that can model a slab of cortex with up to 16x256x256 neurons - over a million! The chips are interconnected in a binary tree by 80M spike/sec links. An on-chip RAM (in each Neurocore) and an off-chip RAM (on a daughterboard, not shown) softwire vertical and horizontcal cortical connections, respectively. It provides an affordable option for brain simulations that uses analog computation to emulate ion-channel activity and uses digital communication to softwire synaptic connections. These technologies impose different constraints, because they operate in parallel and in serial, respectively. Analog computation constrains the number of distinct ion-channel populations that can be simulatedunlike digital computation, which simply takes longer to run bigger simulations. Digital communication constrains the number of synaptic connections that can be activated per secondunlike analog communication, which simply sums additional inputs onto the same wire. Working within these constraints, Neurogrid achieves its goal of simulating multiple cortical areas in real-time by making judicious choices.

Proper citation: Neurogrid (RRID:SCR_005024) Copy   

•   Source: SciCrunch Registry

http://www.bioscreen.fi

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on February 18,2025. Biosceen C is a system designed for automating routine microbiology work. It uses a unique micro-plate format (10x10 wells), so called Honeycomb format, which is especially well suited for highly accurate temperature control. It features two covered honeycomb plates making it possible to run 200 samples simultaneously. Its unique patented incubator system features temperature control maintaining the set temperature with a 0,1 degree centigrade accuracy, while avoiding condensation of liquid on the inside of the micro plate lid. It can cool down the samples 6 degrees centigrade below the ambient temperature and its working range is from 1 to 60 degrees in steps of 0,1 degree centigrade. Sponsors: Oy Growth Curves Ab Ltd is a privately held company established in 2002 by a former Labsystems executive. It aquired the Bioscreen product line from Thermo Labsystems and has continued to maintain the product excellence Bioscreen is known for. Today the company is controlled by a new generation of owner operators, dedicated to continue developing Bioscreen to meet the demands of today''s users Keywords: Microbiology, Automation, Honeycomb, Incubation, System, Temperature, Technology, Supplier,

Proper citation: Bioscreen: C MBR (RRID:SCR_007172) Copy   

•   Source: SciCrunch Registry

http://www.magventure.com

A complete line of non-invasive magnetic stimulation systems designed for clinical examinations and for research in the areas of neurophysiology, neurology, cognitive neuroscience, rehabilitation and psychiatry.

Proper citation: MagVenture: MagPro Magnetic Stimulator (RRID:SCR_009601) Copy   

•   Source: SciCrunch Registry

http://astridbio.com/genominer-genome-analyzer/

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 23,2022. A next generation sequencing data analysis computer for biologists with or without IT background. It has an easy to-use graphical interface to analyze sequencing data in with only 15 clicks. A range of standard, add-on and custom applications help analyze and visualize data generated by Next Generation Sequencing machines. These are installed on each GenoMiner by default: * Reference assembly * De novo assembly * ChiP-Seq * BLAST * Hybrid de novo assembly * Hybrid reference assembly Add-on applications: * Quality assesment * RNA-Seq * Copy Number Variation (CNV) * Multiple Sequence Alignment * miRNA-Seq * Variant Calling

Proper citation: GenoMiner (RRID:SCR_001202) Copy   

•   Source: SciCrunch Registry

https://www.bdbiosciences.com/documents/FACSCalibur_FlowCytometry_TechSpec.pdf

Automated benchtop flow cytometry system. It allows measuring four fluorochrome-conjugates, forward and side scatter., THIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 16,2025.

Proper citation: BD Biosciences: FACSCalibur Flow Cytometry System (RRID:SCR_000401) Copy   

•   Source: SciCrunch Registry

http://www.epson.com/

Commercial vendor of visualization software, factory automation equipment and electric components.

Proper citation: Epson (RRID:SCR_000787) Copy   

•   Source: SciCrunch Registry

http://www.multichannelsystems.com/products/mea2100-systems

In vitro recording system for electrophysiology research with integrated amplifier and stimulus generator, temperature control, and the option for real-time signal detection and feedback. The system can record up to 4 MEAs in parallel at a sampling rate of 50 kHz per channel.

Proper citation: HBio: Multichallel Systems: MEA2100 System (RRID:SCR_014809) Copy   

•   Source: SciCrunch Registry

http://www.pacifictranscription.com.au/philips-dvt-2700.php

Software and accompanying hardware for transcription and voice recording. Software is Windows-compatible only and comes in a physical CD.

Proper citation: Philips DVT2700 Voice Tracer Digital Recorder with Dragon NaturallySpeaking software (RRID:SCR_014800) Copy   

•   Source: SciCrunch Registry

https://www.thermofisher.com/order/catalog/product/LC120

THIS RESOURCE IS NO LONGER IN SERVICE, documented April 10, 2017. It has been succeeded by the Thermo Scientific EASY-nLC 1200 Liquid Chromatography system.

Proper citation: Thermo Fisher: EASY-nLC 1000 Liquid Chromatograph (RRID:SCR_014993) Copy   

•   Source: SciCrunch Registry

https://support.illumina.com/sequencing/sequencing_instruments/nextseq-500.html

High-throughput sequencing machine which enables sequencing of exomes, whole genomes, and transcriptomes. It also provides flow cell types, real-time analysis, and integration with Illumina's BaseSpace computing environment.

Proper citation: Illumina: NextSeq 500 (RRID:SCR_014983) Copy   

•   Source: SciCrunch Registry