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| Resource Name | Proper Citation | Abbreviations | Resource Type |
Description |
Keywords | Resource Relationships | |||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
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WHAM Resource Report Resource Website 100+ mentions |
WHAM (RRID:SCR_005497) | WHAM | software resource | THIS RESOURCE IS NO LONGER IN SERVICE. Documented on February 28,2023. High-throughput sequence alignment tool that aligns short DNA sequences (reads) to the whole human genome at a rate of over 1500 million 60bps reads per hour, which is one to two orders of magnitudes faster than the leading state-of-the-art techniques. Feature list for the current version (v 0.1.5) of WHAM: * Supports paired-end reads * Supports up to 5 errores * Supports alignments with gaps * Supports quality scores for filtering invalid alignments, and sorting valid alignments * finds ALL valid alignments * Supports multi-threading * Supports rich reporting modes * Supports SAM format output | bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: University of Wisconsin-Madison; Wisconsin; USA |
Facebook ; NSF IIS-1110948 |
THIS RESOURCE IS NO LONGER IN SERVICE | OMICS_00697, biotools:wham | https://bio.tools/wham, https://sources.debian.org/src/wham-align/ | SCR_005497 | Wisconsin?s High-throughput Alignment Method | 2026-08-01 12:02:55 | 345 | |||||
|
SeqMap Resource Report Resource Website 50+ mentions |
SeqMap (RRID:SCR_005495) | SeqMap | software resource | A software tool for mapping large amount of oligonucleotide to the genome. It is designed for finding all the places in a genome where an oligonucleotide could potentially come from. SeqMap can efficiently map as many as dozens of millions of short sequences to a genome of several billions of nucleotides. While doing the mapping, several mutations as well as insertions / deletions of the nucleotide bases in the sequences can be tolerated and furthermore detected. Various input and output formats are supported, as well as many command line options for tuning almost every steps in the mapping process. A typical mapping can be done in a few hours on an ordinary PC. | bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: University of Michigan; Ann Arbor; USA |
PMID:18697769 | Free, Non-commercial, Commercial use requires permission | biotools:seqmap, OMICS_00684 | https://bio.tools/seqmap | SCR_005495 | SeqMap - A Tool For Mapping Millions Of Short Sequences To The Genome | 2026-08-01 12:02:59 | 95 | |||||
|
Jellyfish Resource Report Resource Website 1000+ mentions |
Jellyfish (RRID:SCR_005491) | Jellyfish | software resource | A software tool for fast, memory-efficient counting of k-mers in DNA. A k-mer is a substring of length k, and counting the occurrences of all such substrings is a central step in many analyses of DNA sequence. JELLYFISH can count k-mers quickly by using an efficient encoding of a hash table and by exploiting the compare-and-swap CPU instruction to increase parallelism. Jellyfish is a command-line program that reads FASTA and multi-FASTA files containing DNA sequences. It outputs its k-mer counts in an binary format, which can be translated into a human-readable text format using the jellyfish dump command., THIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 16,2025. | c++, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: University of Maryland; Maryland; USA |
PMID:21217122 DOI:10.1093/bioinformatics/btr011 |
THIS RESOURCE IS NO LONGER IN SERVICE | biotools:jellyfish, OMICS_01056 | https://bio.tools/jellyfish, https://sources.debian.org/src/jellyfish1/ | SCR_005491 | Jellyfish mer counter | 2026-08-01 12:02:59 | 1130 | |||||
|
mrFAST Resource Report Resource Website 10+ mentions |
mrFAST (RRID:SCR_005487) | mrFAST | software resource | Software designed to map short reads generated with the Illumina platform to reference genome assemblies; in a fast and memory-efficient mannerl. Currently Supported Features: * Output in SAM format * Indels up to 8 bp (4 bp deletions and 4 bp insertions) * Paired-end mapping ** Discordant option to generate mapping file ready for VariationHunter to detect structural variants. * One end anchored (OEA) map locations for novel sequence insertion detection with NovelSeq * Matepair library mapping (long inserts with RF orientation). Planned Features: * Multithreading | next-generation sequencing, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools is related to: SPLITREAD has parent organization: SourceForge |
PMID:19718026 | biotools:mrfast, OMICS_00671 | https://bio.tools/mrfast | SCR_005487 | mrFAST - Micro Read Fast Alignment Search Tool, Micro Read Fast Alignment Search Tool | 2026-08-01 12:02:54 | 16 | ||||||
|
GNUMAP Resource Report Resource Website 1+ mentions |
GNUMAP (RRID:SCR_005482) | GNUMAP | software resource | THIS RESOURCE IS NO LONGER IN SERVICE. Documented on May 3rd,2023. A software program designed to accurately map sequence data obtained from next-generation sequencing machines (specifically that of Solexa/Illumina) back to a genome of any size. By using the posterior probability of mapping a given read to a specific genomic loation, we are able to account for repetitive reads by distributing them across several regions in the genome. In addition, the output of the program is created in such a way that it can be easily viewed through other free and readily- available programs. Several benchmark data sets were created with spiked-in duplicate regions, and GNUMAP was able to more accurately account for these duplicate regions. | next-generation sequencing, genome, bio.tools |
is listed by: OMICtools is listed by: bio.tools is listed by: Debian has parent organization: Brigham Young University; Utah; USA |
THIS RESOURCE IS NO LONGER IN SERVICE | OMICS_00664, biotools:gnumap | https://bio.tools/gnumap | SCR_005482 | Genomic Next-generation Universal MAPper | 2026-08-01 12:02:53 | 7 | ||||||
|
SAMStat Resource Report Resource Website 10+ mentions |
SAMStat (RRID:SCR_005432) | SAMStat | software resource | C software program for displaying sequence statistics for next generation sequencing. Works with large fasta, fastq and SAM/BAM files. | sequence statistic, c, next generation sequencing, fasta file, fastq file, sam file, bam file, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: RIKEN Yokohama Institute; Kanagawa; Japan |
PMID:21088025 | Acknowledgement requested | biotools:samstat, OMICS_01073 | https://bio.tools/samstat | SCR_005432 | 2026-08-01 12:02:54 | 38 | ||||||
|
MethylViewer Resource Report Resource Website 1+ mentions |
MethylViewer (RRID:SCR_005448) | MethylViewer | software resource | A simple integrated software tool for handling MAP (methyltransferase accessibility protocol) and MAP-IT (MAP individual templates) footprinting projects. It can process sequence data (*.txt, *.ab1 and *.scf) derived from the use of up to four different DNA methyltransferases. | primer design, bisulfite sequencing, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: University of Leeds; West Yorkshire; United Kingdom |
PMID:20959287 | OMICS_00608, biotools:methylviewer | https://bio.tools/methylviewer | SCR_005448 | 2026-08-01 12:02:59 | 2 | |||||||
|
Bis-SNP Resource Report Resource Website 50+ mentions |
Bis-SNP (RRID:SCR_005439) | Bis-SNP | software resource | A software package based on the Genome Analysis Toolkit (GATK) map-reduce framework for genotyping and accurate DNA methylation calling in bisulfite treated massively parallel sequencing (Bisulfite-seq, NOMe-seq, RRBS and any other bisulfite treated sequencing) with Illumina directional library protocol. It contains the following key features: * Call and summarize methylation of any cytosine context provided (CpG, CHH, CHG, GCH et.al.); * Work for single end and paired-end data; * Accurtae variant detection. Enable base quality recalibration and indel calling in bisulfite sequencing; * Based on Java map-reduce framework, allow multi-thread computing. Cross-platform; * Allow multiple output format, detailed VCF files, CpG haplotype reads file for mono-allelic methylation analysis, simplified bedGraph, wig and bed format for visualization in UCSC genome broswer and IGV browser. BisSNP uses bayesian inference with locus specific methylation probabilities and bisulfite conversion rate of different cytosine context(not only CpG, CHH, CHG in Bisulfite-seq, but also GCH et.al. in other bisulfite treated sequencing) to determine genotypes and methylation levels simultaneously., THIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 16,2025. | bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: University of Southern California; Los Angeles; USA |
PMID:22784381 | THIS RESOURCE IS NO LONGER IN SERVICE | biotools:bis-snp, OMICS_00591 | https://bio.tools/bis-snp | SCR_005439 | Bis-SNP - A bisulfite space genotyper & methylation caller, Bis-SNP - A bisulfite space genotyper and methylation caller | 2026-08-01 12:02:59 | 50 | |||||
|
DistMap Resource Report Resource Website 10+ mentions |
DistMap (RRID:SCR_005473) | DistMap | software resource | A user-friendly software pipeline designed to map short reads in a MapReduce framework on a local Hadoop cluster. It is designed to be easily implemented by researchers who do not have expert knowledge of bioinformatics. As it does not have any dependencies, it provides full flexibility and control to the user. The user can use any version of a compatible mapper and any reference genome assembly. There is no need to maintain the mapper, reference or DistMap source code on each of the slaves (nodes) in the Hadoop cluster, making maintenance extremely easy. | mapreduce/hadoop, command line, hadoop cluster, next-generation sequencing, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: University of Veterinary Medicine Vienna; Vienna; Austria has parent organization: Google Code |
PMID:24009693 | GNU General Public License, v3 | OMICS_00660, biotools:distmap | https://bio.tools/distmap | SCR_005473 | 2026-08-01 12:02:59 | 23 | ||||||
|
Stampy Resource Report Resource Website 100+ mentions |
Stampy (RRID:SCR_005504) | Stampy | software resource | A software package for the mapping of short reads from illumina sequencing machines onto a reference genome. It''s recommended for most workflows, including those for genomic resequencing, RNA-Seq and Chip-seq. Stampy excels in the mapping of reads containing that contain sequence variation relative to the reference, in particular for those containing insertions or deletions. It can map reads from a highly divergent species to a reference genome for instance. Stampy achieves high sensitivity and speed by using a fast hashing algorithm and a detailed statistical model. Stampy has the following features: * Maps single, paired-end and mate pair Illumina reads to a reference genome * Fast: about 20 Gbase per hour in hybrid mode (using BWA) * Low memory footprint: 2.7 Gb shared memory for a 3Gbase genome * High sensitivity for indels and divergent reads, up to 10-15% * Low mapping bias for reads with SNPs * Well calibrated mapping quality scores * Input: Fastq and Fasta; gzipped or plain * Output: SAM, Maq''s map file * Optionally calculates per-base alignment posteriors * Optionally processes part of the input * Handles reads of up to 4500 bases | bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: Wellcome Trust Centre for Human Genetics |
PMID:20980556 | OMICS_00691, biotools:stampy | https://bio.tools/stampy | SCR_005504 | 2026-08-01 12:02:59 | 182 | |||||||
|
Scalable Nucleotide Alignment Program Resource Report Resource Website 100+ mentions |
Scalable Nucleotide Alignment Program (RRID:SCR_005501) | SNAP | software resource | A sequence aligner software program that is 10-100x faster and simultaneously more accurate than existing tools like BWA, Bowtie2 and SOAP2. It runs on commodity x86 processors, and supports a rich error model that lets it cheaply match reads with more differences from the reference than other tools. This gives SNAP up to 2x lower error rates than existing tools and lets it match larger mutations that they may miss. SNAP also natively reads BAM, FASTQ, or gzipped FASTQ, and natively writes SAM or BAM, with built-in sorting, duplicate marking, and BAM indexing. | windows, linux, os x |
is listed by: OMICtools is listed by: Debian has parent organization: University of California at Berkeley; Berkeley; USA |
Apache License, 2, Acknowledgement requested | OMICS_00687 | https://sources.debian.org/src/snap-aligner/ | SCR_005501 | SNAP - Scalable Nucleotide Alignment Program | 2026-08-01 12:02:55 | 119 | ||||||
|
NGSView Resource Report Resource Website 1+ mentions |
NGSView (RRID:SCR_005637) | NGSView | software resource | A generally applicable, flexible and extensible next-generation sequence alignment editor. The software allows for visualization and manipulation of millions of sequences simultaneously on a desktop computer, through a graphical interface. | next-generation sequence, alignment, edit, visualization, bio.tools |
is listed by: OMICtools is listed by: bio.tools is listed by: Debian has parent organization: SourceForge |
Acknowledgement requested | biotools:ngsview, OMICS_00891 | https://bio.tools/ngsview | SCR_005637 | 2026-08-01 12:02:57 | 2 | |||||||
|
HiCUP Resource Report Resource Website 100+ mentions |
HiCUP (RRID:SCR_005569) | HiCUP | software resource | A tool for mapping and performing quality control on Hi-C data. | bio.tools |
is listed by: OMICtools is listed by: bio.tools is listed by: Debian has parent organization: Babraham Institute |
OMICS_00523, biotools:hicup | https://bio.tools/hicup | SCR_005569 | Hi-C User Pipeline | 2026-08-01 12:02:56 | 273 | |||||||
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OLego Resource Report Resource Website 10+ mentions |
OLego (RRID:SCR_005811) | OLego | software resource | A program specifically designed for de novo spliced mapping of mRNA-seq reads. It adopts a multiple-seed-and-extend scheme, and does not rely on a separate external mapper. | bio.tools |
is listed by: OMICtools is listed by: bio.tools is listed by: Debian has parent organization: Columbia University; New York; USA |
biotools:olego, OMICS_01244 | https://bio.tools/olego | SCR_005811 | 2026-08-01 12:02:59 | 15 | ||||||||
|
PePr Resource Report Resource Website 50+ mentions |
PePr (RRID:SCR_005759) | PePr | software resource | A ChIP-Seq peak calling or differential binding analysis tool that is primarily designed for data with biological replicates. It uses a negative binomial distribution to model the read counts among the samples in the same group, and look for consistent differences between ChIP and control group or two ChIP groups run under different conditions. | python, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: Google Code |
PMID:24894502 | GNU General Public License, v3 | OMICS_04058, biotools:pepr | https://bio.tools/pepr | SCR_005759 | pepr-chip-seq, Peak Prioritization Pipeline, pepr-chip-seq: A ChIP-Seq analyzing program for biological replicates | 2026-08-01 12:03:02 | 53 | |||||
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Quantitative Enrichment of Sequence Tags Resource Report Resource Website 10+ mentions |
Quantitative Enrichment of Sequence Tags (RRID:SCR_004065) | QuEST | software resource | A Kernel Density Estimator-based package for analysis of massively parallel sequencing data from chromatin immunoprecipitation (ChIP-seq) experiments. | genome-wide, transcription factor binding site, chip-seq, transcription factor, binding site, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools is related to: Gene Ontology has parent organization: Stanford University; Stanford; California |
PMID:19160518 | OMICS_00458, biotools:quest | https://bio.tools/quest | SCR_004065 | Quantitative Enrichment of Sequence Tags: QuEST | 2026-08-01 12:02:37 | 49 | ||||||
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Pash 3.0 Resource Report Resource Website 1+ mentions |
Pash 3.0 (RRID:SCR_004078) | Pash 3.0 | software resource | Performs sequence comparison and read mapping and can be employed as a module within diverse configurable analysis pipelines, including ChIP-Seq and methylome mapping by whole-genome bisulfite sequencing. | bio.tools |
is listed by: OMICtools is listed by: bio.tools is listed by: Debian |
PMID:21092284 | biotools:pash, OMICS_00586 | https://bio.tools/pash | SCR_004078 | 2026-08-01 12:02:37 | 1 | |||||||
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EpiGRAPH Resource Report Resource Website 10+ mentions |
EpiGRAPH (RRID:SCR_004326) | EpiGRAPH | software resource | A software for genome and epigenome analysis., THIS RESOURCE IS NO LONGER IN SERVICE. Documented on September 16,2025. | bio.tools |
is listed by: OMICtools is listed by: bio.tools is listed by: Debian |
THIS RESOURCE IS NO LONGER IN SERVICE | OMICS_00633, biotools:epigraph | https://bio.tools/epigraph | SCR_004326 | 2026-08-01 12:02:35 | 18 | |||||||
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InsertionMapper Resource Report Resource Website |
InsertionMapper (RRID:SCR_004163) | InsertionMapper | software resource | A pipeline tool for the identification of targeted sequences from multidimensional high throughput sequencing data. It consists of four independently working modules: Data Preprocessing, Database Modeling, Dimension Deconvolution and Element Mapping. This pipeline tool is applicable to scenarios requiring analysis of the tremendous output of short reads produced in NGS sequencing experiments of targeted genome sequences. | high throughput sequencing, dna sequence, next generation sequencing, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools has parent organization: SourceForge has parent organization: Montclair State University; New Jersey; USA |
PMID:24090499 | Acknowledgement requested, GNU General Public License | OMICS_01547, biotools:insertionmapper | https://bio.tools/insertionmapper | SCR_004163 | 2026-08-01 12:02:38 | 0 | ||||||
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bcbio-nextgen Resource Report Resource Website 100+ mentions |
bcbio-nextgen (RRID:SCR_004316) | bcbio-nextgen | software resource | A python toolkit providing best-practice pipelines for fully automated high throughput sequencing analysis. | mapreduce/hadoop, bio.tools |
is listed by: OMICtools is listed by: Debian is listed by: bio.tools |
Free, Available for download, Freely available | biotools:bcbio-nextgen, OMICS_01121, BioTools:bcbio-nextgen | https://github.com/chapmanb/bcbb/blob/master/nextgen/README.md, https://bio.tools/bcbio-nextgen, https://bio.tools/bcbio-nextgen | SCR_004316 | 2026-08-01 12:02:39 | 155 |
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