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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpC-1/mpC-1 (mpC-1, V)
Affected Genes: MPC (membranellar pattern C)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Formation of a fourth membranelle in the oral apparatus, with increased penetrance at higher temperature. Located on chromosome 3, probably 3L. The published phenotypic analysis was carrried out on a different homozygous clone that is now extinct.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01229 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E443A, E445A,E446A ]; pm-r, mp-?
, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E443A, E445A, E446A or neo replacing coding of some ATU1 genes
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7 X AAKO5.5 transformed with pATU-E443,445-6A derived from pTUB100E3-PvuII (linearized) where the ATU1 gene is marked with Hae III and Pvu II sites and E443A, E445A,E446A.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01902 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaE-4/cdaE-4 (cdaE-4; cdaE, III)
Affected Genes: CDAE (cell-division-arrest E)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Allelelic to cdaE-1 (IA137) and cdaE-3 (IA225). The extinct cdaE-4 IV sister clone showed identical phenotype, similarly high (possibly higher) expression at 39°C. compared to IA137 (Sister clone to IA312).
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01749 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding
Availability:
References:
Synonyms:
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01908 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAY/htay[∆ ::neo3]; MTT1/mtt1[5’neo2, ∆ :: 2HAn,(HTAY1-258, HTAX1-138)]; pm-r, VII)
Affected Genes: HTAY (TTHERM_01079200) and HTA1 (TTHERM_00790790)
Genomic Alteration: Macronucleus: Neo3 into HTAY, Chimeric YN-HTAX into MTT1 locus
Availability:
References:
Synonyms:
Notes: Shot Xiaoyuan Song’s i∆HTAY strain(partial replacement of HTAY with neo3) with construct MTT1-2HA tags after the ATG of HTAY n-terminus (AA1-258) followed by HTAX AA1-138) also has neo2 in 5’ flank of MTT1.Single cell line (3D) with most or all of HTAY replaced by the HA tagged YN-HTAX.
Pushed to 30mg/ml pm resistance with Cd at 0.1-.0.5 ug/ml needed to express the YN-HTAX
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02201 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 2.* series are separate cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01872 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01871 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mat1-2 (IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A representative of the latest inbred generation of the B strain derived in the Frankel lab (by Leslie Jenkins). It was not used in any crosses with mutant clones.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01755 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TWI1/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, heterozygous
Availability:
References:
Synonyms:
Notes: Heterozygous heterokaryon of TWI1 KO, can mate with ∆TWI1 hehe F3-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02847 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaI-2/cdaI-2 (cdaI-2, VI)
Affected Genes: CDAI (cell division arrest I)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Allelic to cdaI-1 (IA237) and cdaI-3 (IA282). Unequal fission-arrest following an anterior sliding of the oral primordium (39o C). Although 100% penetrant at 39o C., it is the least strongly expressed of the three known cdaI alleles; the anteriorly displaced division furrow often succeeds in completing cytokinesis, producing a small, often amacronucleate, anterior daughter cell.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01515 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding, not sure if complete
Availability:
References:
Synonyms:
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01910 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpA-1/mpA-1 (mpA-1, VII)
Affected Genes: MPA (membranellar pattern A) Originally published as mp/mp
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Abnormal oral apparatuses arising during oral replacement as a consequence of incomplete joining of two membranellar subfields. Not temperature-sensitive. Kaczanowski's original description was of non-mutagenized clones of the inbred D strain, which was accidentally homozygous for the naturally-occurring mpA-1 allele. IA280 is a derived offshoot, and shows very low expression in nutrient medium. Expression under culture conditions conducive to oral replacement was not tested.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01516 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; rts255/rts255 (mpr1-1; cy-s, gal-s, mp-r, IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00947 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wt (pm-r)
Affected Genes: WAG1 (TTHERM_00299879)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: N-terminally tagged WAG1, completely assorted, neo3 cassette, B2086-based strain
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02055 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: rmm11[C3]/rmm11[C3] (mp-s, pm-s, rrm11, III)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: rmm = rDNA maturation or maintenance mutation, located in the rDNA
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01640 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcr2[∆?::neo3]/dcr2[∆?::neo3] (DCR2, ?)
Affected Genes: DCR2 (TTHERM_00316190)
Genomic Alteration: Micronucleus: homozygous KO of DCR2 with neo3
Availability:
References:
Synonyms:
Notes: Homozygous heterokaryon of ∆DCR2 Round I progeny of he∆SAI3 F2-6-pms-1
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02178 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi11[∆?::neo3] (twi11[∆?::neo3])
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: Neo 3 cassette replaces part of TWI11 coding region Macronucleus: Neo 3 cassette replaces part of TWI11 coding region
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon, partial coding seq. were replaced with neo3 cassette. Defective in mitosis
mates with TWI11 KO F2-23,
This strain was supposed to be pm-r but thawed pm-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02858 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1 (CHX1, MPR1; gal1-1; cy-s, mp-s, gal-R, IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01647 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcr1[∆?::neo3]/dcr1[∆?::neo3] (dcr1[∆?], ?)
Affected Genes: DCR1 (TTHERM_01216140)
Genomic Alteration: Micronucleus: homozygous KO of DCR1 with neo3 Macronucleus: Loss of part of DCR1 locus and of the neo3 used to KO.
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon of ∆DCR1. neo3 was deleted in Mac and assorted to pm-s. Can mate with c∆SAI1 3-7-1. DCR1 = SAI1.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01928 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-22; Mild basal body disorganization (TS)., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-22
Availability:
References:
Synonyms:
Notes: Alanine scanning allele rescue of cen1∆ - Cen1 E22A, E23A, K25A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03273 Copy
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