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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hhf1[∆::neo]/hhf1[∆::neo] (mpr1-1; mp-r, ?)
Affected Genes: HHT1 (TTHERM_00570560),HHT2 (TTHERM_00189180), HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo into HHT1, HHF1, HHT2/HHF2
Availability:
References:
Synonyms:
Notes: These are intermediate germline KO heterokaryons. May be early batch and not tested for somatic rescue. Final germline KO strains are #4 and #7. Non-star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02039 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (rnh2[∆::neo3]; pm-r, VI)
Affected Genes: RNH2 (TTHERM_00142290)
Genomic Alteration: Macronucleus: neo3 KO of RNH2
Availability:
References:
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Notes: Somatic KO of RNH2 (RNase H-2) of CU427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02831 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (rnh2[∆::neo3]; pm-r, VII)
Affected Genes: RNH2 (TTHERM_00142290)
Genomic Alteration: Macronucleus: neo3 KO of RNH2
Availability:
References:
Synonyms:
Notes: Somatic KO of RNH2 (RNase H-2) of CU428
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02716 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding
Availability:
References:
Synonyms:
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01906 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes:
Genomic Alteration:
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Notes: Collected by Paul Doerder on 7/29/2008 from the pond designated SG29 in PA (latitude 41.73, longitude -79.23)
Has a micronucleus. Mated with < 6 testers.
Proper citation: RRID:TSC_SD03091 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, D124A,E126A, HAc)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutations D124-A and E126-A and HA tag at c-teminus into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: pDE124/6 HA rescued GTUKO 5x6 progeny having mutation @ D124-A and E126-A in GTU1-1 gene, also one HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02044 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, I)
Affected Genes:
Genomic Alteration:
Availability:
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Synonyms:
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00028 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00033 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr10-1[∆::NEO2] (sfr10-1[∆::NEO2], sfr8-1[∆::MTT-CHX]; Basal body organization defects., ?)
Affected Genes: SFR10 (TTHERM_00392670), SFR8 (TTHERM_00059100)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2] Macronucleus: sfr8-1[∆::MTT-CHX]
Availability:
References:
Synonyms:
Notes: sfr10 complete KO with sfr8::MTT-CHX fully assorted.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03302 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (mlh1[243::neo2]; pm-r, ?)
Affected Genes: MLH1 (TTHERM_00471820)
Genomic Alteration: Macronucleus: Neo2 cassette inserted into coding of MLH1
Availability:
References:
Synonyms:
Notes: Transformation of CU428xB2086, complete knockout of mic histone linker gene with neo2 cassette inserted into Stu1 site. Paromomycin res. to 2 mg/ml Cell lines LH3 and LH6 can mate with each other.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02694 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT?/MAT? (CHX1[C3];pmr1-1; MAT?; cy-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
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Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01245 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (tcd1[5’neo3,FLAG HAn] complete replacement by Southern; pm-r, II)
Affected Genes: HPL2 (TTHERM_01337400)
Genomic Alteration:
Availability:
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Synonyms:
Notes: Neo3 in the 5’ region of CHD2 with Flag-HA tag after the ATG, complete replacement in MAC.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02610 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (chx1-1; PMR1[C3]; MAT3; cy-r, pm-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01256 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (GTU1/gtu1[3’neo2neo2,HAc], VII)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3’ flanking region
Availability:
References:
Synonyms:
Notes: GTU (gamma tubulin) gene with c-terminal HA tag and two neo2 cassette inserted in 3’ flanking region. Partial replacement
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01412 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2] (mpr1-1; mp-r, II)
Affected Genes: HHT2 (TTHERM_00189180) + HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 replaces the HHF2 gene and HHT2 gene and the sequence between them
Availability:
References:
Synonyms:
Notes: The germline knockout heterokaryon of HHT2/HHF2 and the intergenic region has neo cassette completely replacing coding region. 3 independent lines #9, #10, #11.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02623 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, II)
Affected Genes: HHT1 (TTHERM_00570560)
Genomic Alteration: Micronucleus: Neo KO of HHT1
Availability:
References:
Synonyms:
Notes: The germline knockout heterokaryon of HHT1 gene with neo cassette completely replacing coding region. Three independent cell lines #1,#2,# 3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02621 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (twi11[∆?::neo3]; pm-r, VII)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Macronucleus: TWI11 with neo3
Availability:
References:
Synonyms:
Notes: Somatic TWI 11 KO. Part of the coding sequence was replaced by neo3. In CU428
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02864 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
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Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, pm-s, mat3. Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01259 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HHT3?/htt3[5’neo2, ∆, (::HHT2,GFPc)]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: HHT2 with GFP c-terminal tag replaces the HHT3 coding region
Availability:
References:
Synonyms:
Notes: somatic replace H3.3 coding with H3.2+GFP, neo 2 cassette in 5’ flank of H3.3. Not sure if complete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01899 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (pm-r, VII)
Affected Genes: MTT1 (TTHERM_00241640)
Genomic Alteration: Macronucleus: Neo coding replaces MTT1 coding of some of MTT1 copies, both present
Availability:
References:
Synonyms:
Notes: CU428 transformed by pTTMN construct and cells selected with 10 mg/ml paromomycin. Partially replaced. Still has wt Metallothionein gene copies in mac as well as other alleles with neo coding.
Proper citation: RRID:TSC_SD01419 Copy
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