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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cnjB[∆?::neo3]/ cnjB[∆?::neo3]
(cnjB[∆?::neo3], ?)
Affected Genes: CnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: Neo3 replaces part of coding Macronucleus: Neo3 replaces part of coding
Availability:
References:
Synonyms:
Notes: Homozygote CnjB KO Homokaryon. Partial cnjB coding sequences were replaced by neo3.
This strain was supposed to be pm-r but thawed pm-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02761 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi1[∆?::neo3]/twi1[∆?::neo3] (twi1[∆?::neo3]; eri1[∆?::neo3]; same as ∆Twi1)
Affected Genes: ERI1 (TTHERM_00501020)
Genomic Alteration: Micronucleus: TWI1 ko Macronucleus: twi1 kO and ERI1 KO
Availability:
References:
Synonyms:
Notes: Eri1 KO construct was shot into twi1 KO homozygous homokaryon (Neo3 gene is lost in mac, strain is pm-s).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02007 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 8/26/2008 from the pond designated SG69-1 in Guys Mills, PA (lat. 41 37.174, long. -79 55.095, elevation 1549 ft, nearest road is Carpenter Road).
Geographical area: Western Pennsylvania
Collection number: 893
Has a micronucleus.
Cech's self-splicing intron is present. Cytochrome oxidase I haplotype = ANF1
Proper citation: RRID:TSC_SD01556 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT2 (TTHERM_00189180), HHF2 (TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 ko of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01955 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E443D,E445D E446D,E447D]; ATU1 with E443D, E445D,E446D,E447D or neo replacing coding of some ATU1 genes, pm-r, cy-s,mp-?
)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E443D, E445D, E446D, E447D or neo replacing coding of some ATU1 genes
Availability:
References:
Synonyms:
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pATU-4D derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E443D, E445D,E446D,E447D
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01837 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: blt1-1[∆,neo3]/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
Availability:
References:
Synonyms:
Notes: BLT germline heterokaryon, homozyous KO in mic, pm sensitive (120 ug/ml). Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO, Mates with BLTGL1-3,4,5 and 2-5.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02250 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01166 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, HAc(::GTU1, T146V)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with mutation T146V and c-terminus HA tag into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: pT146V-HA rescued GTUKO 5x6 progeny having mutation @ GTU1 T146V gene, driven by MTT1 promoter. Needs Cd to induce. Has HA tag at c-terminus. Single cell (a)
These are same as previous ones, but new transformants, (one week old) not single celled and haven’t tested genotype yet
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02531 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01165 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1, vac?/vac? (mpr1, vac?; mp-r, vac-, oral apparatus-, contractil vacuole pore-, rounded, IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Complementation group could not be determined because of sterility of the mutant.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01603 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpC-2/mpC-2 (mpC-2, VII)
Affected Genes: MPC (membranellar pattern C)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Formation of a fourth membranelle in the oral apparatus. Located on chromosome 3, probably 3L. Allelic to mpC-1 (IA308), with similar oral expression and no pleiotropic effects on cell division. Penetrance increased at higher temperatures.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01451 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mic still from VB1 cell (btu1[∆2,3,4]/btu1[∆2,3,4]; BTU1 has M--- ( REI removed at start of coding); no longer taxol hypersensitive or oryzalin resistant since back to wt L350)
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Macronucleus: Removed the amino acids REI at positions 2, 3, 4 ibeta tubulin 1 gene
Availability:
References:
Synonyms:
Notes: Somatically transformed VB1 cells with BTU1 p94c construct. p94c changes MREI at begining of coding to remove the REI. Also put the missing “C†back into the BTU1 coding region (~ position + 1040)
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02269 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (mpr1-1; mp-r, ?)
Affected Genes: HHT + HHF (TTHERM_00570560, TTHERM_00189180, TTHERM_00189170)
Genomic Alteration: Micronucleus: Neo2 KO of HHT1 and HHT2/HHF2
Availability:
References:
Synonyms:
Notes: This is the germline KO homozygous heterokaryon. Both copies of major histone H3 were removed. Also HHF2 and intergenic region connecting it to HHT2. KO with Neo cassette. The ∆H3 gl 2.* series have a different mating type from the ∆H3 gl 10.* series All the 10.* series are separate cell lines.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02148 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-2/hpo1-2; cdaA-2/cdaA-2 (hpo1-2; cdaA-2; hpo1-2, cdaA-2, III)
Affected Genes: HPO1(hypoangular1), CDAA (cell division arrestA)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: This clone combines a temperature-sensitive fission arrest with a hypoangular phenotype. It was used to generate and investigate "left handed" hypoangular cells
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01579 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (hta1[ K125R]; hta2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTA1 and HTA2 genes Macronucleus: mutated HTA1
Availability:
References:
Synonyms:
Notes: G4B1G10A x G4B3C1A. mutated HTA1 rescue ∆HTA1∆HTA2 double knockout heterokaryon. No H2A.1 ubiquitination . Can mate with D13D1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02390 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (pm-r, VII)
Affected Genes: TTHERM_00238830
Genomic Alteration: Macronucleus: V5 tagged PP36 protein
Availability:
References:
Synonyms:
Notes: somatic mac transformation of CU428 with construct of PP36 (Protein phosphatase 36 kD MW, predicted TTHERM_00238830) V5 tag after ATG, with neo3 in reverse orientation -200 bases upstream of ATG. Localizes to nucleoli, not studied further.
Incomplete replacement, pushed to 3mg/ml pm with 0.5ug/ml Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02273 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaA-1/cdaA-1; cdaE-1/cdaE-1 (cdaA-1; cdaE-1; cdaA, cdaE, II)
Affected Genes: CDAA (cell division arrest A), CDAE (cell division arrest E)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Joint expression of cdaA and cdaE patterns of fission arrest. The published phenotypic analysis was carried out in a sister clone that is no longer available.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01589 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpD-1/mpD-1 (mpD-1, IV)
Affected Genes: MPD (membranellar pattern D)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Formation of four or five well-formed membranelles in the oral apparatus at 39-40 degrees. Located on chromosome 3R; Clean temperature-sensitive expression of oral phenotype associated with cell elongation.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01228 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (htax[S134A,3’neo2]; pm-r, II)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Macronucleus: HTAX has mutation S134A with neo2 cassette in 3’ flank
Availability:
References:
Synonyms:
Notes: Complete somatic transformant with hta1s134-neo2 replacing the HTA1(HTAX) gene in mac of B2086, same as B2086-XS46(6) just different cell line.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01904 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht1[∆::neo2] ; hht2[3’neo2,(S10A, S1028)]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180), HHT1 (TTHERM_00189180)
Genomic Alteration: Macronucleus: HHT1 replaced by neo2, HHT2 has S10A, S28A mutations
Availability:
References:
Synonyms:
Notes: Serine 10 and 28 were mutated to Ala in the coding region of the HHT2 gene. Then the mutated HHT2 gene with its flanking neo marker was transformed into ∆HHT1. Can grow in 100mg/ml paromomycin. Southern blot not yet done to see if complete.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02161 Copy
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