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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00050673
Source Database: WormBase (WB)
Affected Genes: WBGene00006994(zyg-9)
Genomic Alteration: WBGene00006994(zyg-9)
Availability: unknown
Synonyms: zyg-9(or1956[gfp::zyg-9]) II; ltIs37 IV.
Notes: itIs37 [pie-1p::mCherry::H2B::pie-1 3'UTR + unc-119(+)] IV. GFP tag inserted into endogenous zyg-9 locus. Might still contain unc-119(ed3) in the background. Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020|"ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV. GFP tag inserted into endogenous zyg-9 locus. Might still contain unc-119(ed3) in the background. [NOTE: the ltIs37 [pie-1p::mCherry::his-58 + unc-119(+)] IV transgene was previously annotated as itIs37 in this strain. The correct name of the transgene is ltIs37 and not itIs37.] Reference: Chuang CH, et al., Biology Open 2020 9: bio052308 doi: 10.1242/bio.052308 Published 25 June 2020"
Proper citation: RRID:WB-STRAIN:WBStrain00050673 Copy
http://www.wormbase.org/db/get?name=WBStrain00050678
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: gwIs39 gwIs34 unc-119(ed3) III.
Notes: gwIs39 [baf-1::gfp-lacI::let-858 3'UTR + vit-5::GFP] III. gwIs34 [myo-3::mCherry + 256xlacO + unc-119(+)] III. Superficially wild-type. Expresses GFP-LacI throughout development from early embryogenesis, forming a small spot at the lacO array. Worms have red muscle (from L1 stage) and green intestine (from late L4 stage). Reference: Meister P, et al. Genes Dev. 2010 Apr 15;24(8):766-82. PMID: 20395364
Proper citation: RRID:WB-STRAIN:WBStrain00050678 Copy
http://www.wormbase.org/db/get?name=WBStrain00050711
Source Database: WormBase (WB)
Affected Genes: WBGene00003746(nlp-8)
Genomic Alteration: WBGene00003746(nlp-8)
Availability: unknown
Synonyms: nlp-8(syb762) IV.
Notes: Made_by: Marina Sinner /SunyBiotech|"syb762 is a 1076 bp deletion in nlp-8. Flanking sequences: taaaacccggaacc - acttcttgaacaactg Forward primer: TAAAAGCGGAGTAGCGTCCA Reverse primer: CAGATGGTCGGGTGATTTGA syb762 was generated in a mutant background and out-crossed to N2 to remove those background mutations. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791"
Proper citation: RRID:WB-STRAIN:WBStrain00050711 Copy
http://www.wormbase.org/db/get?name=WBStrain00050716
Source Database: WormBase (WB)
Affected Genes: WBGene00004323(rde-1)
Genomic Alteration: WBGene00004323(rde-1)
Availability: unknown
Synonyms: frSi21 II; frIs7 IV; rde-1(ne300) V.
Notes: frSi21 [col-62p::rde-1 3'UTR] II. frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. frSi21 inserted into ttTi5605 site using CRISPR/Cas9 engineering. RDE-1 activity is rescued in adult epidermal tissues, making animals RNAi-deficient except for hypodermal (skin) tissues from the young adult stage. The frSi21 insertion can be detected using a primer within the col-62 promoter (jep2245: caaaaaggcgggatgagcag) in combination with downstream primer in rde-1 (jep2817 tcatactcgtagtattcccg), producing a 965 bp product if insertion is present. rde-1(ne300) can be genotyped by sequencing the PCR product from jep2299: gaacaacgacaatcgagcacca and jep3108: ATcttgtgaccgaactgtcc (jep3108 is not present in the frSi21 transgene). Reference: Watts JS, et al. G3 (Bethesda) 2020 Nov 5;10(11):4167-4176. PMID: 32943454|"frSi21 [col-62p::rde-1 3'UTR] II. frIs7 [nlp-29p::GFP + col-12p::DsRed] IV. frSi21 inserted into ttTi5605 site using CRISPR/Cas9 engineering. RDE-1 activity is rescued in adult epidermal tissues, making animals RNAi-deficient except for hypodermal (skin) tissues from the young adult stage. The frSi21 insertion can be detected using a primer within the inserted vector but outside the genomic rde-1 sequence included in the transgene (jep3108: ATcttgtgaccgaactgtcc) in combination with downstream primer (jep2445: caaaaaggcgggatgagcag), producing a 965 bp product if insertion is present. rde-1(ne300) can be genotyped by sequencing the PCR product from jep2299: gaacaacgacaatcgagcacca and jep3108: ATcttgtgaccgaactgtcc. Reference: https:"
Proper citation: RRID:WB-STRAIN:WBStrain00050716 Copy
http://www.wormbase.org/db/get?name=WBStrain00050714
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:34865044
Synonyms: osIs158 II; ieSi58 IV.
Notes: Made_by: Takefumi Negishi|"osIs158 [eft-3p::ccvTIR-1(F79G)::mRuby] single copy inserted into ttTi5605 on LG II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Negishi T, et al. Genetics. 2021 Dec 2;iyab218. doi: 10.1093/genetics/iyab218."|"osIs158 [eft-3p::ccvTIR-1(F79G)::mRuby] single copy inserted into ttTi5605 on LG II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. This strain expresses the improved version of TIR1 used for improved auxin-inducible degradation (AID2) technology. Reference: Yesbolatova A, et al. Nat Commun. 2020 Nov 11;11(1):5701."
Proper citation: RRID:WB-STRAIN:WBStrain00050714 Copy
http://www.wormbase.org/db/get?name=WBStrain00050713
Source Database: WormBase (WB)
Availability: unknown
Synonyms: cshIs128 II; ieSi58 IV.
Notes: cshIs128 [rps-28p::TIR1::T2A::mCherry::his-11 + Cbr-unc-119(+)] II. ieSi58 [eft-3p::degron::GFP::unc-54 3'UTR + Cbr-unc-119(+)] IV. Companion strain to HML1012. Harbors conventional allele of TIR1 and Nuclear localized mCherry co-expression marker.|"Made_by: Nat"
Proper citation: RRID:WB-STRAIN:WBStrain00050713 Copy
http://www.wormbase.org/db/get?name=WBStrain00050719
Source Database: WormBase (WB)
Availability: unknown
Source References: PMID:38839826
Synonyms: wrdSi46 I.
Notes: wrdSi46 [SCMp*::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (I:-5.32). Seam cell-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in seam cell nuclei. Some expression in hypodermal cells. Reference: Ashley GE, et al. Genetics. 2021 Mar 31;217(3):iyab006. doi: 10.1093/genetics/iyab006. PMID: 33677541|"wrdSi46[SCMp*::TIR1::F2A::BFP::AID*::NLS::tbb-2 3'UTR] I:-5.32 Seam cell-specific expression of TIR1 co-factor for AID, and tissue-specific AID-tagged blue protein in seam cell nuclei. Some expression in hypodermal cells. Reference Ashley et al., 2020"
Proper citation: RRID:WB-STRAIN:WBStrain00050719 Copy
http://www.wormbase.org/db/get?name=WBStrain00050660
Source Database: WormBase (WB)
Affected Genes: WBGene00003564(ncs-2)
Genomic Alteration: WBGene00003564(ncs-2)
Availability: unknown
Source References: PMID:38530893
Synonyms: ncs-2(ju836)
Notes: Superficially wild-type. Reference: Zhou K, et al. Cell Reports 2017 May 9;19(6):1117-1129. PMID: 28494862
Proper citation: RRID:WB-STRAIN:WBStrain00050660 Copy
http://www.wormbase.org/db/get?name=WBStrain00050662
Source Database: WormBase (WB)
Availability: unknown
Synonyms: uqIs5.
Notes: uqIs5 [lbp-2p::lbp-2::TagRFP]. Age-dependent aggregation of LBP-2::TagRFP in the pseudocoelom. Reference: Gallotta I, et al. Nature. 2020 Jul 8. doi: 10.1038/s41586-020-2461-z.
Proper citation: RRID:WB-STRAIN:WBStrain00050662 Copy
http://www.wormbase.org/db/get?name=WBStrain00050661
Source Database: WormBase (WB)
Affected Genes: WBGene00004272(rab-8)
Genomic Alteration: WBGene00004272(rab-8)
Availability: unknown
Synonyms: rab-8(tm2526) I; muIs32 II.
Notes: muIs32 [mec-7p::GFP + lin-15(+)]. Homozygous viable. Reference: Kim KW, et al. Elife. 2018 Nov 21;7:e39756. doi: 10.7554/eLife.39756. PMID: 30461420
Proper citation: RRID:WB-STRAIN:WBStrain00050661 Copy
http://www.wormbase.org/db/get?name=WBStrain00050701
Source Database: WormBase (WB)
Availability: unknown
Synonyms: goeIs397.
Notes: goeIs397 [hsp-16.2p::cnc-10::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-10::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050701 Copy
http://www.wormbase.org/db/get?name=WBStrain00050667
Source Database: WormBase (WB)
Availability: unknown
Synonyms: bmdSi170 I.
Notes: bmdSi170 [loxN::eft-3p::his-58::GFP::3xHA] I. Superficially wild-type. bmdSi170 is a single-copy CRISPR/Cas9-engineered insertion of HIS-58 C-terminally tagged with non-codon-optimized GFP and driven by the ubiquitous eft-3 promoter. Reference: Azmi MA, et al. bioRxiv 2020.10.17.344069; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00050667 Copy
http://www.wormbase.org/db/get?name=WBStrain00050666
Source Database: WormBase (WB)
Availability: unknown
Synonyms: bmdSi141 I.
Notes: bmdSi141 [loxN::eft-3p::his-58::GFP] I. Slow growing. Maintain at 15-20C. bmdSi141 is a single-copy CRISPR/Cas9-engineered insertion of HIS-58 C-terminally tagged with codon-optimized GFP and driven by the ubiquitous eft-3 promoter. Reference: Azmi MA, et al. bioRxiv 2020.10.17.344069; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00050666 Copy
http://www.wormbase.org/db/get?name=WBStrain00050665
Source Database: WormBase (WB)
Availability: unknown
Synonyms: bmdSi147 I.
Notes: bmdSi147 [loxN::rps-27p::DHB::2xmKate2::P2A::H2B::GFP] I. bmdSi147 is a single-copy CRISPR/Cas9-engineered insertion of a codon-optimized CDK sensor (amino acids 9941087 of human DNA Helicase B (DHB) fused to two copies of mKate2) co-expressed with his-58 (H2B) fused to GFP. Reference: Adikes RC, et al. Visualizing the metazoan proliferation-terminal differentiation decision in vivo. bioRxiv 2019.12.18.881888|"Made_by: Taylor N. Medwig-Kinney"
Proper citation: RRID:WB-STRAIN:WBStrain00050665 Copy
http://www.wormbase.org/db/get?name=WBStrain00050709
Source Database: WormBase (WB)
Availability: unknown
Synonyms: goeIs413.
Notes: goeIs413 [hsp-16.2p::cnc-9::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-9::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050709 Copy
http://www.wormbase.org/db/get?name=WBStrain00050708
Source Database: WormBase (WB)
Availability: unknown
Synonyms: goeIs412.
Notes: goeIs412 [hsp-16.2p::cnc-7::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-7::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050708 Copy
http://www.wormbase.org/db/get?name=WBStrain00050707
Source Database: WormBase (WB)
Availability: unknown
Synonyms: goeIs411.
Notes: goeIs411 [hsp-16.2p::cnc-4::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-4::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050707 Copy
http://www.wormbase.org/db/get?name=WBStrain00050650
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00005016(sqt-1)|WBGene00077721(mir-1022)
Genomic Alteration: WBGene00003514(myo-2), WBGene00005016(sqt-1), WBGene00077721(mir-1022)
Availability: unknown
Synonyms: mir-1022(umn51[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) X.
Notes: Made_by: Julie Knott & Marcus Vargas|"mir-1022 pre-miRNA deletion allele in which mir-1022 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.]"
Proper citation: RRID:WB-STRAIN:WBStrain00050650 Copy
http://www.wormbase.org/db/get?name=WBStrain00050657
Source Database: WormBase (WB)
Affected Genes: WBGene00004443(rpl-29)
Genomic Alteration: WBGene00004443(rpl-29)
Availability: unknown
Synonyms: rpl-29(tm3555) IV.
Notes: Homozygous viable Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376.
Proper citation: RRID:WB-STRAIN:WBStrain00050657 Copy
http://www.wormbase.org/db/get?name=WBStrain00050654
Source Database: WormBase (WB)
Affected Genes: WBGene00004140(ebax-1)
Genomic Alteration: WBGene00004140(ebax-1)
Availability: unknown
Synonyms: ebax-1(ju699) IV; juEx1434.
Notes: juEx1434 [rgef-1p::ebax-1::GFP + rol-6(su1006)]. Pick Rollers to maintain. juEx1434 rescues Egl of pqn-55(ju699) mutants. Reference: Wang Z., et al. Neuron. 2013 Sep 4;79(5):903-16.
Proper citation: RRID:WB-STRAIN:WBStrain00050654 Copy
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