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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00040557
Source Database: WormBase (WB)
Affected Genes: WBGene00003822(nsy-1)
Genomic Alteration: WBGene00003822(nsy-1)
Availability: unknown
Synonyms: nsy-1(ok593); rgaIs1; rgaIs2; rgaIs3
Alternate IDs: WB-STRAIN:WSR100
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00040557 Copy
http://www.wormbase.org/db/get?name=WBStrain00040559
Source Database: WormBase (WB)
Availability: unknown
Synonyms: rgaIs5[ttx-3p::G-GECO1.1-T2A-DsRed2; odr1-p::NLS-G-GECO1.1-T2A-NLSDsRed2; lin-44p::DsRedT3]
Alternate IDs: WB-STRAIN:WSR124
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00040559 Copy
http://www.wormbase.org/db/get?name=WBStrain00040560
Source Database: WormBase (WB)
Availability: unknown
Synonyms: rgaIs6[ttx-3p::G-GECO1.1-T2A-DsRed2; odr1-p::NLS-G-GECO1.1-T2A-NLSDsRed2; lin-44p::DsRedT3]
Alternate IDs: WB-STRAIN:WSR125
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00040560 Copy
http://www.wormbase.org/db/get?name=WBStrain00040568
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: PMID:38177158
Synonyms: unc-119(ed3) III; wtEx30.
Alternate IDs: WB-STRAIN:WT30, CGC_WT30
Notes: wtEx30 [cuti-1p::cuti-1::GFP + unc-119(+)]. Maintain by picking non-Unc. Reference: Fritz JA & Behm CA. PLoS One. 2009;4(4):e5117.
Proper citation: RRID:WB-STRAIN:WBStrain00040568 Copy
http://www.wormbase.org/db/get?name=WBStrain00040564
Source Database: WormBase (WB)
Affected Genes: WBGene00044508(nsy-7)
Genomic Alteration: WBGene00044508(nsy-7)
Availability: unknown
Synonyms: nsy-7 (tm3080); rgaIs5
Alternate IDs: WB-STRAIN:WSR129
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00040564 Copy
http://www.wormbase.org/db/get?name=WBStrain00040563
Source Database: WormBase (WB)
Availability: unknown
Synonyms: kyIs408; rgaIs5
Alternate IDs: WB-STRAIN:WSR128
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00040563 Copy
http://www.wormbase.org/db/get?name=WBStrain00040565
Source Database: WormBase (WB)
Availability: unknown
Synonyms: kyIs408/+; rgaIs5/+
Alternate IDs: WB-STRAIN:WSR131
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00040565 Copy
http://www.wormbase.org/db/get?name=WBStrain00040539
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: unc-119(ed3) III; opIs206.
Alternate IDs: WB-STRAIN:WS4274, CGC_WS4274
Notes: opIs206 [hif-1p::hif-1(genomic)::GFP::hif-1 3'UTR + unc-119(+)]. Weak GFP signal in early embryonic stages (2-cell, 4-cell, 8-cell, etc.). Reference: Sendoel A, et al. Nature. 2010 Jun 3;465(7298):577-83.
Proper citation: RRID:WB-STRAIN:WBStrain00040539 Copy
http://www.wormbase.org/db/get?name=WBStrain00040537
Source Database: WormBase (WB)
Affected Genes: WBGene00000499(chk-2)
Genomic Alteration: WBGene00000499(chk-2)
Availability: available
Source References: PMID:36176234
Synonyms: chk-2(gk212) V.
Alternate IDs: WB-STRAIN:WS3455, CGC_WS3455
Notes: Made_by:|"Supplementary_genotype chk-2(gk212)"
Proper citation: RRID:WB-STRAIN:WBStrain00040537 Copy
http://www.wormbase.org/db/get?name=WBStrain00040530
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00002042(hus-1)
Genomic Alteration: WBGene00000254(bli-4), WBGene00002042(hus-1)
Availability: available
Synonyms: hus-1(op244) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:WS2265, CGC_WS2265
Notes: Heterozygotes are WT GFP+ and segregate non-glowing hus-1 homozygotes and very rare homozygous hT2 glowing animals, and dead eggs. qIs48 is an insertion of ccEx9747 with markers: myo-2::GFP expressed brightly in the pharynx throughout development, pes-10::GFP expressed in embryos, and gut promoter driving GFP in the intestine. hus-1(op244) mutants from homozygous parents show an incompletely penetrant maternal effect embryonic lethality. Note: qIs48 has been observed to recombine off hT2, typically leaving behind a functional homozygous viable hT2 with Bli-4 phenotype.
Proper citation: RRID:WB-STRAIN:WBStrain00040530 Copy
http://www.wormbase.org/db/get?name=WBStrain00040533
Source Database: WormBase (WB)
Affected Genes: WBGene00011376(gla-3)
Genomic Alteration: WBGene00011376(gla-3)
Availability: available
Synonyms: gla-3(op212) I.
Alternate IDs: WB-STRAIN:WS2973, CGC_WS2973
Notes: Made_by: Ekat Kritikou|"Sterile or nearly sterile. Extra programmed cell death in germline. Maintain at 20 C. Reference: Kritikou et al. 2006 Genes Dev 20(16):2279-92."
Proper citation: RRID:WB-STRAIN:WBStrain00040533 Copy
http://www.wormbase.org/db/get?name=WBStrain00040532
Source Database: WormBase (WB)
Affected Genes: WBGene00011376(gla-3)
Genomic Alteration: WBGene00011376(gla-3)
Availability: available
Synonyms: gla-3(op216) I.
Alternate IDs: WB-STRAIN:WS2972, CGC_WS2972
Notes: Made_by: Ekat Kritikou|"Sterile or nearly sterile. Extra programmed cell death in germline. Maintain at 20 C. Reference: Kritikou et al. 2006 Genes Dev 20(16):2279-92."
Proper citation: RRID:WB-STRAIN:WBStrain00040532 Copy
http://www.wormbase.org/db/get?name=WBStrain00040540
Source Database: WormBase (WB)
Availability: available
Synonyms: opIs257.
Alternate IDs: WB-STRAIN:WS4543, CGC_WS4543
Notes: opIs257 [rad-54p::rad-54::YFP::rad-54 3'UTR + unc-119(+)].
Proper citation: RRID:WB-STRAIN:WBStrain00040540 Copy
http://www.wormbase.org/db/get?name=WBStrain00040545
Source Database: WormBase (WB)
Availability: available
Synonyms: opIs310.
Alternate IDs: WB-STRAIN:WS4918, CGC_WS4918
Notes: opIs310 [ced-1p::YFP::act-5::let-858 3'UTR + unc-119(+)].
Proper citation: RRID:WB-STRAIN:WBStrain00040545 Copy
http://www.wormbase.org/db/get?name=WBStrain00040548
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)|WBGene00015310(fan-1)
Genomic Alteration: WBGene00006843(unc-119), WBGene00015310(fan-1)
Availability: available
Synonyms: unc-119(ed3) III; fan-1(tm423) IV; opIs406.
Alternate IDs: WB-STRAIN:WS5229, CGC_WS5229
Notes: opIs406 [fan-1p::fan-1::GFP::let-858 3'UTR + unc-119(+)]. opIs406 resuces tm423. Reference: Kratz K, et al. Cell. 2010 Jul 9;142(1):77-88.
Proper citation: RRID:WB-STRAIN:WBStrain00040548 Copy
http://www.wormbase.org/db/get?name=WBStrain00040547
Source Database: WormBase (WB)
Affected Genes: WBGene00000390(cdc-42)
Genomic Alteration: WBGene00000390(cdc-42)
Availability: available
Synonyms: cdc-42(gk388) opIs295 II.
Alternate IDs: WB-STRAIN:WS5018, CGC_WS5018
Notes: opIs295 [cdc-42p::GFP::cdc-42(genomic)::cdc-42 3'UTR + unc-119(+)] II. opIs295 fully rescues cdc-42 animals.
Proper citation: RRID:WB-STRAIN:WBStrain00040547 Copy
http://www.wormbase.org/db/get?name=WBStrain00040542
Source Database: WormBase (WB)
Affected Genes: WBGene00002003(hse-5)|WBGene00006853(unc-130)
Genomic Alteration: WBGene00002003(hse-5), WBGene00006853(unc-130)
Availability: available
Synonyms: unc-130(op459) II; hse-5(tm472) III.
Alternate IDs: WB-STRAIN:WS4591, CGC_WS4591
Notes: EMPTY
Proper citation: RRID:WB-STRAIN:WBStrain00040542 Copy
http://www.wormbase.org/db/get?name=WBStrain00040544
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Synonyms: opIs295 II; unc-119(ed3) III.
Alternate IDs: WB-STRAIN:WS4700, CGC_WS4700
Notes: opIs295 [cdc-42p::GFP::cdc-42(genomic)::cdc-42 3'UTR + unc-119(+)] II.
Proper citation: RRID:WB-STRAIN:WBStrain00040544 Copy
http://www.wormbase.org/db/get?name=WBStrain00040639
Source Database: WormBase (WB)
Availability: available
Synonyms: qaIs8400.
Alternate IDs: WB-STRAIN:XA8400, CGC_XA8400
Notes: qaIs8400 [let-858p::Ov-GST-3 + rol-6(su1006)]. Called AK1 in the reference article. The Ov-GST-3 gene was amplified from genomic DNA of O. volvulus with 1M of the sequence specific primer 5'Klon and 3'Klon (5'Klon: 5'-GGCGTACGATGTCAAGATTTCCTCAACAAG-3'; 3'Klon: 5'-GGTCTAGATTTATTTAGGAATGATTGAATCGGTCG-3'; representing bases 4 - 25 and the complementary sequence of bases 821 - 841 of the published Ov-GST-3 cDNA (AF203814); bold underlines indicate restriction sites for Pfl23II (SplI) and XbaI, respectively; dotted underline indicates the start codon for translation; italics indicates the conserved sequence for the polyadenylation signal for transgenic transcript processing; the 8 5'-nucleotides of primer 3'Klon and the fourteen 5'-nucleotides of primer 5'Klon do not correspond to the template and introduce the sequences to the amplicon), 200 M of each deoxynucleotide (Gibco BRL) and 2.5 units of Taq polymerase (Gibco BRL). After an initial denaturation of 3 minutes at 93C, 35 cycles of annealing at 55C for 1 minute, synthesis at 72C for 2 minutes and a 1 minute denaturation at 93C were performed, followed by a final extension at 72C for 5 minutes. The genomic Ov-GST-3 fragment obtained by PCR (see above) was ligated into the pGEM-T Easy vector (Promega) by TA-cloning, cleaved with the restriction enzymes Pfl23II (SplI) and XbaI (restriction sites introduced by the primer) and inserted between the unique Pfl23II (SplI) and XbaI sites of the vector pPD103.05 (kindly provided by A. Fire). The sequence of the genomic Ov-GST-3 fragment in the resulting plasmid pAK1 was confirmed by automated dye terminator, dideoxy sequencing (ABI Prism 377TM Sequencer, PE Applied Biosystems) using the PCR primers (see above). The pAK1 DNA was injected in combination with the marker plasmid pRF4 [rol-6(su1006)] into the gonads of N2 C. elegans at a concentration of approximately 100 ng/l for each plasmid. Transgenic worms were identified by the selectable Roller marker phenotype and the stable transmitting line AK1ex (AK1 extrachromosomal) was established. Integration of the extrachromosomal arrays was achieved by irradiation of AK1ex worms with 3600 rad (1 rad = 0.01 Gy) of x-rays (x-ray chamber: RUM 9421-070-77002, Philips, Netherlands; dosimeter: PTW-SN4, PTW, Germany). The progeny of these worms was then screened for 100% transmittance of the Roller phenotype to obtain the C. elegans line AK1int (AK1 integrated) with the chromosomally integrated transgenes.
Proper citation: RRID:WB-STRAIN:WBStrain00040639 Copy
http://www.wormbase.org/db/get?name=WBStrain00040635
Source Database: WormBase (WB)
Affected Genes: WBGene00001593(glc-3)
Genomic Alteration: WBGene00001593(glc-3)
Availability: available
Synonyms: glc-3(ok321) V.
Alternate IDs: WB-STRAIN:XA7400, CGC_XA7400
Notes: Mutagen:UV/TMP|"ZC317.3 Homozygous. Outer Left Sequence: TCAAAATACAGGGGTAGGCG. Outer Right Sequence: ACAATTCCTGGAACTCACGG. Inner Left Sequence: TGAAGAGGTTTTGAAACGCA. Inner Right Sequence: ACTTTCCGAGAGGAATGGGT. Inner Length: 2746. Estimated Deletion Size: 1200. This strain was provided by the C. elegans Gene Knockout Project at OMRF, which is part of the International C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. URL: www.mutantfactory.ouhsc.edu/"
Proper citation: RRID:WB-STRAIN:WBStrain00040635 Copy
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