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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT3; cy-r, I)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01962 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pdd2[∆?::neo2], ?)
Affected Genes: PDD2 (TTHERM_00283530)
Genomic Alteration: Macronucleus: PDD2 with neo2 replacing the 5’ part of coding
Availability:
References:
Synonyms:
Notes: Progeny of B2086XCU428 transformation. Somatic KO of PDD2 gene.
Mates with CU427, CU428, ∆PDD2-som-BC1, BC3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01323 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CnjB/cnjB[∆?::neo3] (cnjB[∆?::neo3]
CnjB:neo3, fully assorted; pm-r)
Affected Genes: cnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: Part of CnjB coding replaced with neo3 Macronucleus: Part of CnjB coding replaced with neo3, completely assorted
Availability:
References:
Synonyms:
Notes: somatic knock-outs of CnjB obtained by mating heterozygous CnjB KO heterokaryon with WT then assorted to complete KO in mac; mate with ∆CnjB #3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01845 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CnjB/cnjB[∆?::neo3] (?)
Affected Genes: CnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: CnjB has part of coding replaced with neo3
Availability:
References:
Synonyms:
Notes: Heterozygous heterkaryon for ∆CnjB. Mates with Tomoko’s ∆CnjB 2-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02780 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi1[∆?::neo3]/twi1[∆?::neo3] (twi1[∆?::neo3]; eri1[∆?::neo3]; same as ∆Twi1, ?)
Affected Genes: ERI1 (TTHERM_00501020)
Genomic Alteration: Micronucleus: TWI1 KO Macronucleus: twi1 kO and ERI1 KO
Availability:
References:
Synonyms:
Notes: Eri1 KO construct was shot into twi1 KO homozygous homokaryon (Neo3 gene is lost in mac, strain is pm-s).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02025 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-r, pm-s, mat3
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01297 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (V)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Collected by Paul Doerder on 7/22/1994 from the pond designated SG29 in PA (latitude 41.73, longitude -79.23)
Presence of micronucleus not determined.
Proper citation: RRID:TSC_SD03115 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: in CU427, chx1-1/chx1-1 (drh1[∆?::neo3]; DRH1 gene 5’ end of coding replaced with Neo3 ,pm-r up to 700ug/ml pm with Cd., VI)
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Macronucleus: Neo3 cassette replaces part of 5’ end of coding
Availability:
References:
Synonyms:
Notes: Somatic KO of DRH1 with neo3 KO2 construct. 5’ end of coding removed. Bad maters so not used
See Jody DRH1 logbook
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryons do not grow well.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01850 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mat1-2/mat1-2; serH-3/serH-3 (mat1-2; serH-3, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Inbred B clones were originally generated in studies on mating-type determination; B1868 = 18th generation of inbreeding, most recent inbreeding cross in 1968.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01619 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (pm-r, VII)
Affected Genes: TTHERM_00238830
Genomic Alteration: Macronucleus: V5 tagged PP36 protein
Availability:
References:
Synonyms:
Notes: somatic mac transformation of CU428 with construct of PP36 (Protein phosphatase 36 kD MW, predicted TTHERM_00238830) V5 tag after ATG, with neo3 in reverse orientation -200 bases upstream of ATG. Localizes to nucleoli, not studied further.
Incomplete replacement, pushed to 3mg/ml pm with 0.5ug/ml Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02271 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (HTA1 (now HTAX) is WT but HTA2 (now HTA1) has neo2, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes Macronucleus: WT HTAX
Availability:
References:
Synonyms:
Notes: G4B1G10A x G4B3C1A. wt HTA1 (pXL53) rescue ∆HTA1∆HTA2 double knockout heterokaryon. Assort to pm-s. Mates with HTA1-D1A01, D2B01, D3E01
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02395 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: con2-1/con2-1 (con2-1, V)
Affected Genes: CON2 (conical2)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Conical shape; expression slightly more pronounced at 39° than at 28° C. Division products are equal in size.
Very similar shape to the much more studied con1-1 mutant (J. Exp. Zool. 192: 237-258), but differs in that division products are approximately equal in size, indicating that conical shape does not per se lead to smaller posterior daughter cells.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01462 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cdaA-3/cdaA-3 (cdaA-3; cdaA, III)
Affected Genes: CDAA (cell division arrest A)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A relatively weakly expressed mutant allele of cdaA, in which cytokinesis is only partially arrested at 39oC; it is the only cdaA mutant allele derived from EMS mutagenesis.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01586 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (LSD2/lsd2-1[Δ1-302(::neo4, KanR2)]; pm-r, II)
Affected Genes: LSD2 (TTHERM_00600670)
Genomic Alteration: Micronucleus: PAR1 gene partially replaced by neo4-KanR2 cassette. Macronucleus: LSD2 gene partially replaced by neo4-KanR2 cassette.
Availability:
References:
Synonyms:
Notes: The knockout also deletes 838bp of the 5' flanking region and introduces a frameshift mutation. The knockout has been confirmed by paromomycin resistance, but macronuclear allele homozygosity has not been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.
Submitted by Robert Coyne, J. Craig Venter Institute
Proper citation: RRID:TSC_SD01740 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pgp1[∆?::neo3]/pgp1[∆?::neo3] (VI)
Affected Genes: PGP1
Genomic Alteration: Micronucleus: neo3 into PGP1 locus
Availability:
References:
Synonyms:
Notes: Possible polyglycine protein gene was knocked out with neo3 cassette. This is a PPG1 KO heterokaryon with homozygous mic KO and mac assorted to wt.This strain can mate with PPG1∆1 and PPG1∆3 cells. Final name of gene is PGP1 when published.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02034 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: disD-1/disD-1 (disD-1, IV)
Affected Genes: DISD (disorganized-D)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Disorganized ciliary rows at 39-40 degrees C. Straightforward t.s. expression of disorganized phenotype,cell division normal.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01222 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (dcl1-?[3’neo3,HAc]/dcl1-?[3’neo3,HAc], VII)
Affected Genes: DCL1 (TTHERM_00284230)
Genomic Alteration: Macronucleus: Mac-DCL1 was completely replaced with DCL1-HA- with neo3 in 3’ flanking region
Availability:
References:
Synonyms:
Notes: Mac-DCL1 was completely replaced with DCL1-HA-neo3, CU428. DCL1 = SAI 2, the original name.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02832 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht2[3’ neo2,FLAGc]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: c-terminal HHT2 FLAG tagged
Availability:
References:
Synonyms:
Notes: Don’t know if complete replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02313 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Availability:
References:
Synonyms:
Notes: homozygous, Neo cassette knockout before we knew there was HHT4
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01865 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (htax[S134A,3’neo2]; pm-r, II)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Macronucleus: HTAX has mutation S134A with neo2 cassette in 3’ flank
Availability:
References:
Synonyms:
Notes: Complete somatic transformant with hta1s134-neo2 replacing the HTA1(HTAX) gene in mac of B2086.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01903 Copy
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