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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2]; htaz[∆::neo2]/htaz[∆::neo2] (mp-r?, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 knockout of HTAX, HTA1, HTAZ
Availability:
References:
Synonyms:
Notes: HTA/HTZ1 triple KO heterokaryons;From mating of h∆∆HTA-G6A and h∆HTZ1-G311A1; made with B*VII
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02633 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[5’neo2,∆,::(HHT2,GFPc)]/hht3[5’neo2,∆,(::HHT2,GFPc)] (?)
Affected Genes: HHT3 (TTHERM_00016170), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: GFP tagged HHT2 into HHT3 locus
Availability:
References:
Synonyms:
Notes: non-star side of H3.2+GFP germline replacement heterokaryon.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01946 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/HTB1 ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: HTB1
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by HTB1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02088 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2; CHX1/CHX1; mpr1?/mpr1? (mp-r, ?)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: Neo2 cassette knockout of HTAX coding region
Availability:
References:
Synonyms:
Notes: HTA1 Germline knockout heterokaryon. Transformed CU428 X B2086 with pQR10B (removes all coding, replaces with neo cassette). It can mate with HTA1 G1 14B,
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01395 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: blt1-1[∆,neo3]/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
Availability:
References:
Synonyms:
Notes: BLT germline heterokaryon, homozyous KO in mic, pm sensitive (120 ug/ml) Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO, Mates with BLTGL5-1 and 5-2.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02246 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K4R] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4R
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-K4R.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02093 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hpo1-4/hpo1-4 (hpo1-4, IV)
Affected Genes: HPO1 (hypoangular)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A complex phenotype with contractile vacuole pores too close to the oral apparatus, and clockwise slippage of oral primordia. Similar to hpo1-1, though much less extensively studied. A sister clone to IA480.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01443 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S10A,S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1, HHT2, HHF1 Macronucleus: KO of HHT1, HHF1, and for HHT2 have mutations S10A and S28A
Availability:
References:
Synonyms:
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S10AS28A #6-later after seq.transformant found a wrong nucleotide~25 bases upsteam of ATG, transformant seems normal.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02376 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/hhf2,hht2[∆,::neo2] (hht1[∆::neo2]; hhf2,hht2[∆,::neo2]/ hht2[S28A]; pm-r, ?)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo knockout of HHT1, HHT2, HHF2 Macronucleus: KO of HHT1, HHF2, and for HHT2 have mutation S28A
Availability:
References:
Synonyms:
Notes: Mate Yifan Liu’s ∆H3 2-6 x ∆H3 10-6 homozygous heterokaryons (∆HHT1 with neo, and ∆HHT2/HHF2 with neo2 in mic) assorted to Paromomycin sensitive in mac. Shoot with pHHT2S28A#9.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02379 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT3; cy-r, I)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01969 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pm-r, II)
Affected Genes: ASI2 (TTHERM_00191480)
Genomic Alteration: Macronucleus: ASI2 replaced with ASI2Y607F
Availability:
References:
Synonyms:
Notes: Y607 is in a putative SH2 binding site. ASI2 is nonessential for vegetative growth. Matings between ASI2Y607F somatic replacement strains have reduced fertility and delayed endocycling during MAC development.
For paromomycin resistance, add 0.5 ug/ul CdCl2 along with the parmomycin.
Proper citation: RRID:TSC_SD03073 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (chx1-1; cy-r, mp-r, ory-r, pm-r, tax-r, ?)
Affected Genes:
Genomic Alteration: Micronucleus: btu1-1::btu1-1M350K/btu1-1::btu1-1M350K (homozygous) Macronucleus: btu1-1::btu1-1M350K
HHF1::neo2
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00480 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (twi1[3’neo3, FLAG-HA]; pm-r, VI)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: FLAG-HA tagged TWI1 with neo3 replaced all TWI1
Availability:
References:
Synonyms:
Notes: All somatic TWI1 was replaced by FLAG-HA-TWI1 construct. CU427 background.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02782 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: bcd1-2/bcd1-2 (bcd1-2; bcd1, V)
Affected Genes: BCD1 (broadened cortical domains)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A mutation that brings about a broadening of oral and CVP domains. Allelic with bcd 1-1 (clones IA342 and IA344). Exhibits conjugal block, primarily due to failure of nuclear fusion.
Proper citation: RRID:TSC_SD00642 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT2; cy-r, IV)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01977 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT4/hht4[∆, HHT3, 5’neo2,FLAGc]; pm-r, VII)
Affected Genes: HTT4 (TTHERM_00016200), HHT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminalFlag tagged HHT3 put into HHT4 locus, not sure if complete replacement
Availability:
References:
Synonyms:
Notes: Mac transformant. Somatic replace HHT4 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01857 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (pdd2[∆?::neo2]; Progeny of B2086XCU428 transformation
Somatic KO of PDD2 gene, ?)
Affected Genes: PDD2 (TTHERM_00283530)
Genomic Alteration: Macronucleus: PDD2 with neo2 replacing the 5’ part of coding
Availability:
References:
Synonyms:
Notes: Mates with CU427, CU428, ∆PDD2-som-BC4, BC-5.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02032 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2; CHX!/CHX1; mpr1?/mpr1? (mp-r, VII)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: Neo2 cassette knockout of HTAX coding region
Availability:
References:
Synonyms:
Notes: HTA1 Germline knockout heterokaryon. Transformed CU428 X B2086 with pQR10B (removes all coding, replaces with neo cassette). It can mate with HTA1 G1 15B.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02671 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janB-1/janB-1 (janB-1, III)
Affected Genes: JANB (janus B)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: janus phenotype: partially reversed oral apparatus on the dorsal surface; two contractile-vacuole pore sets. Also the only temperature-sensitive janus mutant. There is very little expression of the janus phenotype at 28° C, strong expression at 39°.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01461 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Availability:
References:
Synonyms:
Notes: homozygous, Neo cassette knockout before we knew there was HHT4
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01862 Copy
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