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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1, vacA/vacA (mpr1, vacA; mp-r, vac-, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01599 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K130R]; pm-r, mp-r, II)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: neo2 replaces HTAZ coding Macronucleus: HTAZ coding either neo2 or mutated to K130R
Availability:
References:
Synonyms:
Notes: New batch, rescue of h∆HTZ1-G304A1 and G311A1.
This strain was listed as pm-r but is now pm-s.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02689 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (ema1[3’neo3, FLAGc]; pm-r, VI)
Affected Genes: EMA1 (TTHERM_00088150)
Genomic Alteration: Macronucleus: FLAG tagged EMA1 with neo3 in 3’ flanking region
Availability:
References:
Synonyms:
Notes: Somatic DRH26 was replaced by DRH26-FLAG. CU427 background.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02603 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: rmm10null[C3], mono1L/4L; chx1-1/CHX1[C3], mpr1-1/MPR1[C3] (chx1-1;mpr1-1; pm-s, cy-r, mp-r, rrm10, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: rmm = rDNA maturation or maintenance mutation, located in the rDNA
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01639 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1; Nulli 5 (MPR1; mp-r, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00827 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (btu1[∆::H4-neo]/btu1[∆::H4-neo]; pm-r, VII)
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Macronucleus: ∆BTU1 with H4 driven neo coding
Availability:
References:
Synonyms:
Notes: Complete disruption of BTU1 by an H4-neo cassette.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01916 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ? (Partial replacement CDC2/cdc2-? CDC2 gene 5’ flanking sequence of 247 bp with GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking.; pm-r with Cd+2, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: CDC2 gene has 5’ flanking sequence of 247 bp and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking.
Availability:
References:
Synonyms:
Notes: XS72 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. In CDC2 locus, coding region replaced by GFP coding. Shortened CDC2 5’ flanking region is 247 bases. Neo3 cassette is in 3’ flanking region.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02575 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (HTB1/htb1[A8C, K4R]; htb2 [∆::neo2] assorted to pm-s so must have lost neo2; HTB1with A8C, K4R, ∆htb2, pm-s, cy-s, mp-r,, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with A8C, K4R mutation
Availability:
References:
Synonyms:
Notes: New batch, rescue of progeny h∆∆HTB86 B4A6 and D2A2 with HTB1 with A8C, K4R mutation
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01884 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; MPR1[C3]/MPR1[C3]; pmr1-1/pmr1/1; MAT2/MAT2 (CHX1[C3]; cy-s, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-s, pm-r, mat 2
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01246 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (btu1[∆::H4-neo]/btu1[∆::H4-neo]; pm-r
, VII)
Affected Genes: BTU1 (TTHERM_00348510)
Genomic Alteration: Macronucleus: ∆BTU1 with H4 driven neo coding
Availability:
References:
Synonyms:
Notes: Complete disruption of BTU1 by an H4-neo cassette.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01920 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (SNF2/snf2[5’neo3,HAHAn], II)
Affected Genes: SNF2 (TTHERM_00388250)
Genomic Alteration: Macronucleus: Two HA tags after ATG of SNF2 coding
Availability:
References:
Synonyms:
Notes: 2ha-tag was put in next to ATG of snf2. snf2 2ha-neo3 construct (4.8kb) was shot into B2086 . B2086 WT mac of snf2 were replaced by snf- 2ha partly. Localized in mic of conjugation
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02186 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; MPR1[C3]/MPR1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];MPR1[C3]; pmr1-1; MAT2; cy-s, mp-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01255 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; MPR1[C3]/MPR1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];MPR1[C3]; pmr1-1; MAT2; cy-s, mp-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01257 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (GTU1/gtu1[3’neo2neo2,HAc], VII)
Affected Genes: GTU1 (TTHERM_00079520 )
Genomic Alteration: Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3’ flanking region
Availability:
References:
Synonyms:
Notes: GTU (gamma tubulin) gene with c-terminal HA tag and two neo2 cassette inserted in 3’ flanking region. Partial replacement.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01414 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-134; Basal body disorganization and stability defects (TS), ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-134
Availability:
References:
Synonyms:
Notes: Alanine scanning allele rescue of cen1∆ - Cen1 E134A, E135A, E136A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03282 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K4R,K10R,K13R,K16R,K21R]; pm-r, ?)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutations K4R, K10R, K13R, K16R, K21R
Availability:
References:
Synonyms:
Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ. mutated RKRRRR. Slow growth, ts at 40C
Proper citation: RRID:TSC_SD02076 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];pmr1-1; MAT2; cy-s, pm-r, ?)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01300 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAY?/htay[∆::neo3]; MTT1/mtt1[5’neo2, ∆, 2HAn(::HTAY)]; pm-r, VII)
Affected Genes: H2A.Y (TTHERM_01079200)
Genomic Alteration: Macronucleus: Neo3 KO of HTAY(may be incomplete) and HTAY with two HA tags on the N-terminus inserted into MTT1 locus with neo2 cassete in 5’ flanking region
Availability:
References:
Synonyms:
Notes: Transformed pXS83 (MTT-neo2-2HA-HTAY-MTT) into HTA.Y real somatic knockout strain in MTT1 locus.Selected in the presence of Cd +Pm. PCR shows very faint band of WT HTA.Y and WT MTT1 copy left (might come from mic).
Grow cells 0.5 ug/ml Cd and .5 mg/ml pm and increase that up to 6 mg/ml with passage.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01786 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (HTA3/hta3[∆::neo2], ?)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Macronucleus: neo2 into HTA3
Availability:
References:
Synonyms:
Notes: CET of CU428 x B2086 with plamid XL203, HTA3 with neo2 cassette replacing coding
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02874 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wi1[∆::neo3]/twi1[∆::neo3] (twi1[∆::neo3] with rDNA::pD5H8-PDD1-GFP, ?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, homozygous Macronucleus: TWI1 knockout
Availability:
References:
Synonyms:
Notes: Homozygous homokaryon of TWI1 KO with PDD1-GFP expressed from the rDNA vector.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02758 Copy
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