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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01117 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, I)
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00027 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, I)
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00029 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: wild type C3 (wild type C3; wild type C3, II)
Notes: See Figure 1 on page 13 of Methods in Cell Biology Volume 62, Tetrahymena thermophila 2000 (Editors D.J. Asai and J.D. Forney) for and excellent diagram explaining how the inbred strains are related to each other.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD00030 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr10-1[∆::NEO2] (Mild basal body organization defects., ?)
Affected Genes: SFR10 (TTHERM_00392670)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2]
Source References: PMID:23426847
Notes: sfr10 complete KO.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03301 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: TWI1/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 into TWI1, heterozygous
Source References: PMID:12297043
Notes: Heterozygous heterokaryon of ∆TWI1 progeny of hehe∆TWI1 F3-1 x CU427; assorted to pm-s; can mate with c1-1, c2-1, c6-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02851 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi1[∆::neo3]/?;TWI11/twi11,[neo3,HA] (twi1[∆::neo3]; pm-r, ?)
Affected Genes: TWI1 (TTHERM_01161040), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: Neo3 into TWI1, HA tagged TWI11 Macronucleus: Neo3 into TWI1, HA tagged TWI 11?
Source References: PMID:12297043
Notes: Mates with ∆ Twil HATll-T16. In the mac. all copies are ∆ Twil.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02730 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: possible heterozygote BSR KO (bsr-r, mp-r)
Affected Genes: MLH
Notes: Selected after germline shoot of mic LH-BSR construct. The genotype is to be confirmed after maturing and testing. Same as Mic LH BSR GLKO- LH2 , independent strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01405 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, (T35A,S43A,S45A,T47A, T54A, K29Q,K60Q,K87Q,K117Q,K150Q), GFPc]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to alanine (charge patch) and 5 other sites to glutamine, GFP at c-terminus
Source References: PMID:11972045, PMID:10983971
Notes: Complete somatic tranformant of CU428 cells with p5Q /neo construct. Phosphorylation sites 1- 5 of H1 changed to alanines and 5 other sites changed to glutamines,GFP at c-terminus, neo 2 cassette in 3’ flanking region for selection. See paper
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02218 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: WAG1/wag1[∆::neo3]; TWI11/twi11[neo3,HAn] (wag1[∆::neo3]; TWI11/HA tagged TWI11; pm-r, ?)
Affected Genes: WAG1 (TTHERM_00299879), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: heterozygous KO of WAG1 and TWI11, TWI1 also HA tagged Macronucleus: KO of WAG1 with neo3, HA tagged TWI11
Source References: PMID:19596782
Notes: mates with ∆ WAG1 HAT11 W7
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02735 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; PMR1[C3]/PMR1[C3]; MAT3/MAT3 (CHX1[C3]; cy-s, ?)
Source References: PMID:8725229, PMID:8601476
Notes: This strain must be crossed to A* for two rounds and cy-r progeny selected to generate the meiotic segregant panel member which is cy-r, mp-r, pm-s, mat3
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01250 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?HHT3/hht3[3’neo2, HAn]; pm-r, II)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal HA tag of HHT3, not sure if complete replacement
Source References: PMID:16908532, PMID:9343391
Notes: HA-tagged hv2 mac transformant. Checked by western blot. Hahv2 is expressed. This strain can be used to check HA-hv3 localization in conjugated cells by mating with HA-hv2/CU428.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01892 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht4[∆::neo2]/hht4[∆::neo2]
Affected Genes: HHT4 (TTHERM_00016200)
Genomic Alteration: Micronucleus: HHT4 is replaced by neo2
Source References: PMID:16908532
Notes: Germline HHT4 KO heterokaryon HHT4 coding is replaced by neo2, * side
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01377 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, II)
Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01651 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo2,∆,HAHAn(::TCD4)]; pm-r, VII)
Affected Genes: HPL4 (TTHERM_00585180)
Genomic Alteration: Macronucleus: Two HA tags after ATG of TCD4, neo2 in 5’ flank, inserted into MTT1 locus for over expression, need Cd
Notes: HA2-TCD4 overexpession from MTT1 locus, neo 2 upstream. HA-Tcd3p localizes to new mac. TTHERM_00585190
Adjacent to TCD3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02500 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/gal1-1; tyr-9/tyr-9 (gal1-1; tyr-9; gal-r; tyr auxotroph, II)
Source References: PMID:6950403
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01535 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/CHX1; PMR1/pmr1-1; GAL1/gal1-1; CNJ1/cnj1-1 (?; ?, II)
Notes: Macronucleus phenotype and genotype are undetermined.
Proper citation: RRID:TSC_SD00203 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, (T35A,S43A,S45A,T47A, T54A, K33Q,K38Q,K40Q,K50Q,K51Q)]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to alanine (charge patch) and 5 other sites to glutamine.
Source References: PMID:11972045, PMID:10983971
Notes: Complete somatic tranformant of CU428 cells with pQ5A5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to alanines and 5 other sites changed to glutamines, neo 2 cassette in 3’ flanking region for selection. See paper.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02504 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2] (hta1[∆::neo2], htax[∆::neo2]/htax [S1P,,K5R,K8R,K10R, K12R,K17R, S122A, k123R, S124A, K125R, K126R, T127A, S129A]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: HTAX and HTA1 KO with neo Macronucleus: Rescue with mutated HTAX with S1P, , K5R, K8R, K10R, K12R, K17R, S122A, k123R, S124A, K125R, K126R, T127A, S129A
Source References: PMID:17242195
Notes: HTAX S1P+5R+4A+3R mutation somatic rescue strain of HTA1, HTA2 double knockout.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02198 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-EF2; None., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-EF2
Source References: PMID:21562224
Notes: EF-hand mutant allele rescue of cen1∆ - D72A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03285 Copy
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