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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00037547
Source Database: WormBase (WB)
Affected Genes: WBGene00018123(F36H12.9)
Genomic Alteration: WBGene00018123(F36H12.9)
Availability: available
Synonyms: F36H12.9(gk1123) IV.
Alternate IDs: WB-STRAIN:VC2795, CGC_VC2795
Notes: F36H12.9. Identified by PCR, validated by CGH. External left primer: TGTTGTGGAAGTGCAAGAGG. External right primer: CGTATCGGTTAGTCGGCATT. Internal left primer: GCCTCAGCGATATGGAGAAG. Internal right primer: AGAAATCCTTTGTCGATGCG. Internal WT amplicon: 1008 bp. Deletion size: 761 bp. Deletion left flank: TACTCCGCCTCAGCGATATGGAGAAGTTTG. Deletion right flank: ATATATTGTTTTCAGTACTTGGATACTCTT.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037547 Copy
http://www.wormbase.org/db/get?name=WBStrain00037546
Source Database: WormBase (WB)
Affected Genes: WBGene00020388(T10B5.2)
Genomic Alteration: WBGene00020388(T10B5.2)
Availability: available
Synonyms: T10B5.2(gk1153) V.
Alternate IDs: WB-STRAIN:VC2793, CGC_VC2793
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"T10B5.2. External left primer: CTCGGTTTGTACCATGGCTT. External right primer: AATTTTGCGTATTGCGAACC. Internal left primer: GATCTTCCTCATCGTGCCAT. Internal right primer: AGGACATCCGGGAGAGACTT. Internal WT amplicon: 2414 bp. Deletion size: 851 bp. Deletion left flank: AAATGATAGAAGGTCTGCTGGTACTGTGTT. Deletion right flank: GTCCCTCCATCCATCTTCGATATTTTTGGT. Insertion Sequence: TTGTTTGTGT."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037546 Copy
http://www.wormbase.org/db/get?name=WBStrain00037550
Source Database: WormBase (WB)
Affected Genes: WBGene00005724(srv-13)|WBGene00010772(K11D9.3)|WBGene00011327(hlh-34)
Genomic Alteration: WBGene00005724(srv-13), WBGene00010772(K11D9.3), WBGene00011327(hlh-34)
Availability: available
Synonyms: K11D9.3(gk3223) III; srv-13(gk3224) IV; hlh-34(gk1211) V.
Alternate IDs: WB-STRAIN:VC2802, CGC_VC2802
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain is homozygous for a deletion (gk1211) in T01D3.2, detectable by PCR using the following primers. External left primer: GTGAAGCCGAAGGATCATGT. External right primer: CGTCTTTGCTTTCTTTTCCG. Internal left primer: GAAGAACTTTGCATCGAGGG. Internal right primer: TGTCCAACAATTTCCAACGA. Internal WT amplicon: 1737 bp. Deletion size: 301 bp. Deletion left flank: TTAAAAAACAGAAAAAAAATTAAAAATATA. Deletion right flank: CATCTCCGCGCCTGTCCAGTATCACAAAGA. Validation: gk1211 passed by CGH. Other deletions (gk3223, gk3224) identified by CGH."|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037550 Copy
http://www.wormbase.org/db/get?name=WBStrain00037554
Source Database: WormBase (WB)
Affected Genes: WBGene00001072(dpy-10)|WBGene00008860(romo-1)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00008860(romo-1)
Availability: available
Synonyms: F15D4.3(ok3521)/mT1 II; +/mT1 [dpy-10(e128)] III.
Alternate IDs: WB-STRAIN:VC2819, CGC_VC2819
Notes: F15D4.3. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3521 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: AGATTCGGCAAGAGAGGTCA. External right primer: AAAGTTTTGCTCCTGTGCGT. Internal left primer: TAATAATCCCTTGAGCCCCC. Internal right primer: AACGATTTCTTTCACAAAGTGGA. Internal WT amplicon: 1187 bp. Deletion size: 378 bp. Deletion left flank: CTTCTCTTCTCCCTGTGTGTACCAGTGTAC. Deletion right flank: TCGAATCTGGAAATTTTGAAAATAAATTAG.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037554 Copy
http://www.wormbase.org/db/get?name=WBStrain00037552
Source Database: WormBase (WB)
Affected Genes: WBGene00013727(Y111B2A.1)
Genomic Alteration: WBGene00013727(Y111B2A.1)
Availability: available
Synonyms: Y111B2A.1(gk1164) III.
Alternate IDs: WB-STRAIN:VC2805, CGC_VC2805
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y111B2A.1. External left primer: GAAGCTCGAAGAGTGGGATG. External right primer: AGTGTATGCAGCGTGTTTGC. Internal left primer: CCTCTTTGAATTACCGCCAA. Internal right primer: TTTCAGATGAAACGTGCGAG. Internal WT amplicon: 2262 bp. Deletion size: 614 bp. Deletion left flank: TTAATTAATTTCACTGATTTACGCCTGTAA. Deletion right flank: AAAATTGTTTCCAGCCGCTGCGACAATGAT."
Proper citation: RRID:WB-STRAIN:WBStrain00037552 Copy
http://www.wormbase.org/db/get?name=WBStrain00037558
Source Database: WormBase (WB)
Affected Genes: WBGene00001072(dpy-10)|WBGene00003133(apc-1)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00003133(apc-1)
Availability: available
Source References: PMID:38302462
Synonyms: C09H10.7(ok2466)/mIn1 [mIs14 dpy-10(e128)] II.
Alternate IDs: WB-STRAIN:VC2826, CGC_VC2826
Notes: C009H10.7. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok2466 homozygotes (sterile adult, no eggs). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: CAAATTTCCAGGTTCGTCGT. External right primer: TTCCTGTTCGAAACGAGGTT. Internal left primer: GTGGATGCTCCAACTGACAA. Internal right primer: TGACGATTTGAATGTCTGATACAA. Internal WT amplicon: 1330 bp. Deletion size: 550 bp. Deletion left flank: TATACTTGTATGAGTGAAGAATTTGATGAT. Deletion right flank: TCATCCAGCGAACAAACCTTCCACCATCAC. Insertion Sequence: CCATCGGA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037558 Copy
http://www.wormbase.org/db/get?name=WBStrain00037559
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00013580(Y79H2A.3)
Genomic Alteration: WBGene00000254(bli-4), WBGene00013580(Y79H2A.3)
Availability: available
Source References: PMID:38302462
Synonyms: Y79H2A.3(gk1219) III/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2828, CGC_VC2828
Notes: Mutagen:UV/TMP|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y79H2A.3. Maternal-effect lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP gk1219 homozygotes (Mel; F2 homozygotes arrest as early larvae). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AAACATGCTTCTTCCATGCC. External right primer: AGCGAAATTTGGACTAGCGA. Internal left primer: TTCATTGCGTGATATTCCGA. Internal right primer: TCTGGACGTGTGCTACTTGC. Internal WT amplicon: 1396 bp. Deletion size: 1073 bp. Deletion left flank: GTTCATCACCAGCATTAATGAGATATCGAT. Deletion right flank: TAGCTAATTTTGAACCGCCATAAAACTTTT."
Proper citation: RRID:WB-STRAIN:WBStrain00037559 Copy
http://www.wormbase.org/db/get?name=WBStrain00037556
Source Database: WormBase (WB)
Affected Genes: WBGene00000254(bli-4)|WBGene00010419(atp-1)
Genomic Alteration: WBGene00000254(bli-4), WBGene00010419(atp-1)
Availability: available
Source References: PMID:38302462
Synonyms: H28O16.1(ok2203) I/hT2 [bli-4(e937) let-?(q782) qIs48] (I;III).
Alternate IDs: WB-STRAIN:VC2824, CGC_VC2824
Notes: H28O16.1. Homozygous lethal deletion chromosome balanced by bli-4- and GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested hT2 aneuploids, and non-GFP ok2203 homozygotes (probable embryonic arrest). Homozygous hT2[bli-4 let-? qIs48] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: AAATCCTGACAGCTCGTTGG. External right primer: TTCGAAACAGGAGCTTTGCT. Internal left primer: TGTTGTCCAAACGCATTGTT. Internal right primer: ATTCTCGCAGAACACACACG. Internal WT amplicon: 2289 bp. Deletion size: 1121 bp. Deletion left flank: GACGTGTTGTTGACGCCCTCGGAAACCCAA. Deletion right flank: ATACCTCGACAAGGTCGACCCATCCGCCAT. Insertion Sequence: A.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037556 Copy
http://www.wormbase.org/db/get?name=WBStrain00037562
Source Database: WormBase (WB)
Affected Genes: WBGene00001072(dpy-10)|WBGene00022721(ugtp-1)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00022721(ugtp-1)
Availability: available
Synonyms: +/mT1 II; ugtp-1(ok3492)/mT1 [dpy-10(e128)] III.
Alternate IDs: WB-STRAIN:VC2837, CGC_VC2837
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"ZK370.7. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3492 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: CCAATCCGTTTCTGTCGTCT. External right primer: ATGATGCTCTTTCTCGGTCG. Internal left primer: TTGGCGAGAATTTATGAGCC. Internal right primer: TCGATGGATGGCAATTACAC. Internal WT amplicon: 1168 bp. Deletion size: 505 bp. Deletion left flank: TTAAGTTTATACAATTAAAGCTTTTGGCTA. Deletion right flank: TTTTTCAAACGATTTGAAAAAAAAACCCTA."
Proper citation: RRID:WB-STRAIN:WBStrain00037562 Copy
http://www.wormbase.org/db/get?name=WBStrain00037560
Source Database: WormBase (WB)
Affected Genes: WBGene00003056(lon-2)|WBGene00006757(unc-18)
Genomic Alteration: WBGene00003056(lon-2), WBGene00006757(unc-18)
Availability: available
Synonyms: +/szT1 [lon-2(e678)] I; unc-18(ok3477)/szT1 X.
Alternate IDs: WB-STRAIN:VC2835, CGC_VC2835
Notes: F27D9.1. Homozygous viable deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3477 homozygotes (Unc). Pick WT and check for correct segregation of progeny to maintain. External left primer: GGTGGTCTGACATCGAACCT. External right primer: GGGGCTCTGAAAATGAAACA. Internal left primer: GAATTGCTGAACAAATCGCA. Internal right primer: GGGTTGAAATGAGCAATCATC. Internal WT amplicon: 1331 bp. Deletion size: 371 bp. Deletion left flank: TTACTCTTCAAGCAATGTGCTACGACCTTT. Deletion right flank: CAGTATCAACAAGGAGTTGACAAGTTGTGT. Insertion Sequence: AGACCTT.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037560 Copy
http://www.wormbase.org/db/get?name=WBStrain00037529
Source Database: WormBase (WB)
Affected Genes: WBGene00013994(ZK524.4)
Genomic Alteration: WBGene00013994(ZK524.4)
Availability: available
Synonyms: ZK524.4(gk1212) I.
Alternate IDs: WB-STRAIN:VC2760, CGC_VC2760
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZK524.4. Identified by PCR, validated by CGH. External left primer: GAAGTACCTGCTGCTTTGCC. External right primer: TATATGCAACTGCGCTCCAG. Internal left primer: GCTATTGCTCCAGCAACCAT. Internal right primer: TATGTCAAATGCGCCTGAAA. Internal WT amplicon: 1629 bp. Deletion size: 823 bp. Deletion left flank: AGCATATACAAAATAACACCTAATGACCAT. Deletion right flank: CCCTGATGTGCAACGATGATTTTCGGCGGA. Insertion Sequence: GTTCAGCATGGTCAAATATAC."
Proper citation: RRID:WB-STRAIN:WBStrain00037529 Copy
http://www.wormbase.org/db/get?name=WBStrain00037527
Source Database: WormBase (WB)
Affected Genes: WBGene00008487(F01D4.3)
Genomic Alteration: WBGene00008487(F01D4.3)
Availability: available
Synonyms: F01D4.3(gk1221) IV.
Alternate IDs: WB-STRAIN:VC2755, CGC_VC2755
Notes: F01D4.3. Identified by PCR, validated by CGH. External left primer: TCCTCCAATGGTGGTTGACT. External right primer: CCGGATGGAGACAAAAAGAA. Internal left primer: ATCACTTGCTCCGGTTTCAC. Internal right primer: CCAATTCAGTCTGATGGCAA. Internal WT amplicon: 1179 bp. Deletion size: 505 bp. Deletion left flank: TTTCTCCGCAATCGGTACAACAGTTCCAGT. Deletion right flank: CGCTATTCCAAATACATTTTTCTTTTCAGT. Insertion Sequence: TT.|"Made_by: Vancouver KO Group"|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037527 Copy
http://www.wormbase.org/db/get?name=WBStrain00037521
Source Database: WormBase (WB)
Affected Genes: WBGene00020048(lgc-12)
Genomic Alteration: WBGene00020048(lgc-12)
Availability: available
Synonyms: lgc-12(ok3546) III.
Alternate IDs: WB-STRAIN:VC2740, CGC_VC2740
Notes: R13A5.4. External left primer: AGCGAGAGCTGGTGAAACAT. External right primer: CTCGGACAATTTTCGCGTAT. Internal left primer: CCAATTTACTCGACCTGTAAAAA. Internal right primer: TGCATCAAATTAGGTGTCCG. Internal WT amplicon: 1216 bp. Deletion size: 744 bp. Deletion left flank: ACGTAAGTTATGGTAAATAACATACTTTTT. Deletion right flank: GCAATACCGTTCCAGCATTTTCACAGTTAA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037521 Copy
http://www.wormbase.org/db/get?name=WBStrain00037522
Source Database: WormBase (WB)
Affected Genes: WBGene00001488(frm-1)
Genomic Alteration: WBGene00001488(frm-1)
Availability: available
Synonyms: frm-1(gk1225) I.
Alternate IDs: WB-STRAIN:VC2741, CGC_VC2741
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"ZK270.2. Identified by PCR, validated by CGH. External left primer: AATGGTGACACGATGCTCAA. External right primer: ACACAGACACAGCAAGACGG. Internal left primer: GTTAAATTCCAGTGGCTGCG. Internal right primer: GAAGCCGATGGACAAAGAGA. Internal WT amplicon: 796 bp. Deletion size: 98 bp. Deletion left flank: AAGTGATCATTCGACCTTTAAAAGTGATGT. Deletion right flank: TTTGGGTGTACCAGTTAGATATATTGGGGT."
Proper citation: RRID:WB-STRAIN:WBStrain00037522 Copy
http://www.wormbase.org/db/get?name=WBStrain00037520
Source Database: WormBase (WB)
Affected Genes: WBGene00003056(lon-2)|WBGene00006839(unc-115)
Genomic Alteration: WBGene00003056(lon-2), WBGene00006839(unc-115)
Availability: available
Synonyms: +/szT1 [lon-2(e678)] I; unc-115(ok2640)/szT1 X.
Alternate IDs: WB-STRAIN:VC2739, CGC_VC2739
Notes: F09B9.2. Homozygous viable deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok2640 homozygotes (Unc). Pick WT and check for correct segregation of progeny to maintain. External left primer: TCTCATTTTGGTGACGGTGA. External right primer: AAAGGGCAATGAGTTTGCAC. Internal left primer: AGACGAGATCTGGCATCCAT. Internal right primer: GAGAAGAAGAAAAGGCGCAC. Internal WT amplicon: 1358 bp. Deletion size: 512 bp. Deletion left flank: GCAGAATAAAAATTAAAAAAAAATGTTTAA. Deletion right flank: TTGAATCAGTAGCTGGCTATAGAGCACAAC.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037520 Copy
http://www.wormbase.org/db/get?name=WBStrain00037525
Source Database: WormBase (WB)
Affected Genes: WBGene00018488(acs-1)
Genomic Alteration: WBGene00018488(acs-1)
Availability: available
Synonyms: acs-1(gk3066) V/nT1 [qIs51] (IV;V).
Alternate IDs: WB-STRAIN:VC2744, CGC_VC2744
Notes: F46E10.1. Homozygous lethal deletion chromosome balanced by GFP-marked translocation. Heterozygotes are WT with pharyngeal GFP signal, and segregate WT GFP, arrested nT1[qIs51] aneuploids, and non-GFP gk3066 homozygotes (early larval arrest). Homozygous nT1[qIs51] inviable. Pick WT GFP and check for correct segregation of progeny to maintain. External left primer: CTTCGATCAGCAGTTGACCA. External right primer: CAAAGTTGGCAATGGTTGTG. Internal left primer: CAACACAGTTTGCCAGTGCT. Internal right primer: GAGACGACTTGCTGGAGACC. Internal WT amplicon: 2067 bp. Deletion size: 880 bp. Deletion left flank: TTTATTTTAAAAAATATTTAAAAAGTTTTA. Deletion right flank: TATGACTGACATGCAAGTATGCTATGGAAC.|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037525 Copy
http://www.wormbase.org/db/get?name=WBStrain00037526
Source Database: WormBase (WB)
Affected Genes: WBGene00001063(dpy-1)|WBGene00002025(hsp-60)
Genomic Alteration: WBGene00001063(dpy-1), WBGene00002025(hsp-60)
Availability: available
Synonyms: hsp-60(ok3508)/sC1 [dpy-1(s2170)] III.
Alternate IDs: WB-STRAIN:VC2754, CGC_VC2754
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"Y22D7AL.5. Apparent homozygous lethal deletion chromosome balanced by dpy-1-marked recombination suppressor. Heterozygotes are WT, and segregate WT, Dpy (sC1 homozygotes), and ok3508 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: AAATTGATTTTTCCCGCTGA. External right primer: AGGGGAAAAAGAGCCGTAAA. Internal left primer: GAAATTTTGGTTTTCCTGCG. Internal right primer: CAAATGGCTCAGAGCACAAA. Internal WT amplicon: 1227 bp. Deletion size: 611 bp. Deletion left flank: AAAAATTTGAATTTTTCGTGAAAATTTGAA. Deletion right flank: GCTCTCAATCTCTCATTGAAATAACGACAC."
Proper citation: RRID:WB-STRAIN:WBStrain00037526 Copy
http://www.wormbase.org/db/get?name=WBStrain00037538
Source Database: WormBase (WB)
Affected Genes: WBGene00001072(dpy-10)|WBGene00003928(pas-7)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00003928(pas-7)
Availability: available
Synonyms: pas-7(ok3447)/mIn1 [mIs14 dpy-10(e128)] II.
Alternate IDs: WB-STRAIN:VC2777, CGC_VC2777
Notes: This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.|"ZK945.2. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok3447 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TGTGATGATCGAGGAAGCAG. External right primer: TTCGTCTCTCCCGTAAATCG. Internal left primer: AAGCAGTTGCCGCATAACTT. Internal right primer: AACGGTTCTTCTGATTTCCG. Internal WT amplicon: 1263 bp. Deletion size: 409 bp. Deletion left flank: GAATTGTGCATAAACATGTTTCTGGTTTGT. Deletion right flank: ATTCACATCCAGCTCCTCGATCTTCAGCTT."
Proper citation: RRID:WB-STRAIN:WBStrain00037538 Copy
http://www.wormbase.org/db/get?name=WBStrain00037533
Source Database: WormBase (WB)
Affected Genes: WBGene00012731(Y39G8C.2)
Genomic Alteration: WBGene00012731(Y39G8C.2)
Availability: available
Synonyms: Y39G8C.2(gk1099) II.
Alternate IDs: WB-STRAIN:VC2771, CGC_VC2771
Notes: Made_by: Vancouver KO Group|"Mutagen:UV/TMP"|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."|"Y39G8C.2, K03H6.5. External left primer: AAAGAGTTGAAGGCAGGCAA. External right primer: TGAGGAAAATGACCGAAAGG. Internal left primer: GTCGAGCAGCAGGTAGAACC. Internal right primer: GCAAGTTTCCGGAATTGAAA. Internal WT amplicon: 2604 bp. Deletion size: 1081 bp. Deletion left flank: CGCCGGCGCTCGAGCGTTTTAGCGTGCCGA. Deletion right flank: CTTTGTGTACTGCGGCCGACGCTGCACGGG."
Proper citation: RRID:WB-STRAIN:WBStrain00037533 Copy
http://www.wormbase.org/db/get?name=WBStrain00037537
Source Database: WormBase (WB)
Affected Genes: WBGene00001072(dpy-10)|WBGene00004392(rnr-2)
Genomic Alteration: WBGene00001072(dpy-10), WBGene00004392(rnr-2)
Availability: available
Synonyms: +/mT1 II; rnr-2(ok3357)/mT1 [dpy-10(e128)] III.
Alternate IDs: WB-STRAIN:VC2776, CGC_VC2776
Notes: C03C10.3. Apparent homozygous lethal deletion chromosome balanced by dpy-10-marked translocation. Heterozygotes are WT, and segregate WT, arrested mT1 aneuploids, sterile Dpys (mT1 homozygotes), and ok3357 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: GTCTCTCGGCTTCATTCACC. External right primer: GTGTAAAGTCCGCGAAGAGG. Internal left primer: ATACTCGGAAACCCGCTTCT. Internal right primer: ATGCCTTCGAATTTACAGCC. Internal WT amplicon: 1159 bp. Deletion size: 691 bp. Deletion left flank: TTCGATGGCCACAGCGTCCTTGATGATATC. Deletion right flank: TGCCTTTTTGTAGAAGTTCCAGATGTCATG. Insertion Sequence: ATTGATGA.|"This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use."
Proper citation: RRID:WB-STRAIN:WBStrain00037537 Copy
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