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http://www.wormbase.org/db/get?name=WBStrain00047268

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)|WBGene00012354(cox-4)
Genomic Alteration: WBGene00006843(unc-119), WBGene00012354(cox-4)
Availability: unknown
Synonyms: cox-4(zu476[cox-4::eGFP::3XFLAG]) I; egxSi136 II; unc-119(ed3) III.
Notes: egxSi136 [mex-5p::tomm-20::halotag::pie-1 3UTR + unc-119(+)] II. GFP and 3xFLAG tags inserted into endogenous cox-4 locus to create a C-terminal translational GFP fusion. Outer membranes are stably labeled with the TOMM-20::Halotag transgene, and the mitochondria matrix are labeled with COX-4::GFP. Reference: Fan X, et al. G3 (accepted).|"Made_by: Stephanie Miller"

Proper citation: RRID:WB-STRAIN:WBStrain00047268 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047269

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:37708895
Synonyms: egxSi152 II; unc-119(ed3) III.
Notes: egxSi152 [mex-5p: tomm-20::gfp::pie-1 3UTR + unc-119(+)] II. Superficially wild-type. Stable expression of TOMM-20 tagged with GFP on the outer membranes of mitochondria in the germline and embryos. Reference: Fan X, et al. G3 (accepted).|"Made_by: Xintao Fan"|"Supplementary_genotype egxSi152[mex-5p: tomm-20::gfp::pie-1 3UTR + unc-119(+)] II; unc-119(ed3) III"

Proper citation: RRID:WB-STRAIN:WBStrain00047269 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047264

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: egxSi126 I; unc-119(ed3) III
Notes: egxSi126 [mex-5p::hsp-3(aa1-19)::halotag::HDEL::pie-1 3UTR + unc-119(+)] I. Superficially wild-type. Stable expression of Halotag in the ER lumen in the germline and embryos. Reference: Fan X, et al. G3 (accepted).|"Made_by: Xintao Fan"

Proper citation: RRID:WB-STRAIN:WBStrain00047264 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047326

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: qSi369 II; unc-119(ed3) III; qSi370 V.
Notes: qSi369 [sygl-1p::24xMS2 loops::3xflag::sygl-1::sygl1 3'UTR]. qSi370 [mex-5p:: MS2 Coat Protein::linker::sfGFP::tbb-2 3' UTR::gpd-2 intergenic sequence::H2B::mCherry::unc-54 3' UTR]. Superficially wild-type with expression of sfGFP and nuclear mCherry in germline. qSi369 and qSi370 constitute an MS2 system which allows live visualization of sygl-1 nascent transcripts in the C. elegans germline in a glp-1 mutant background. qSi370 can be prone to silencing, especially after severe starvation; silencing of GFP or mCherry expression can occur independently of one another. Maintain by picking animals with bright GFP and mCherry expression. Reference: Lee C, et al. Dev Cell. 2019 Aug 19;50(4):426-435.e4.

Proper citation: RRID:WB-STRAIN:WBStrain00047326 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047420

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: unc-119 (tm4063) III; wgIs797.
Notes: Made_by: DKV/MC|"Mutagen:Particle Bombardment"|"wgIs797 [flt-1::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44."

Proper citation: RRID:WB-STRAIN:WBStrain00047420 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047422

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: unc-119 (tm4063) III; wgIs809.
Notes: Made_by: DKV/MC|"Mutagen:Particle Bombardment"|"wgIs809 [ets-5::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44."

Proper citation: RRID:WB-STRAIN:WBStrain00047422 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047423

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: unc-119 (tm4063) III; wgIs810.
Notes: Made_by: DKV/MC|"Mutagen:Particle Bombardment"|"wgIs810 [C06A6.2::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44."

Proper citation: RRID:WB-STRAIN:WBStrain00047423 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047435

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: unc-119(ed4) III; syEx1539.
Notes: syEx1539 [nhr-246p::GFP + unc-119(+)]. Pick non-Unc to maintain. Dauer decision marker that indicates commitment to dauer formation in intestine and hypodermis. Reference: Shih, PY, et al., Dev Biol. 2019 Jun 23. pii: S0012-1606(18)30788-7.

Proper citation: RRID:WB-STRAIN:WBStrain00047435 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047384

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:32255430, PMID:37728486, PMID:38177158
Synonyms: unc-119(ed3) III; qyIs46.
Notes: qyIs46 [emb-9p::emb-9::mCherry + unc-119(+)] X. Superficially wild-type with very low penetrance (~5%) rupture. Integrated collagen::mCherry reporter. Reference: Ihara S, et al. Nat Cell Biol. 2011 Jun;13(6):641-51.|"Supplementary_genotype [emb-9p::emb-9::mCherry + unc-119(+)] X"

Proper citation: RRID:WB-STRAIN:WBStrain00047384 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047419

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: unc-119 (tm4063) III; wgIs782.
Notes: Made_by: DKV/MC|"Mutagen:Particle Bombardment"|"wgIs782 [ztf-14::TY1::EGFP::3xFLAG + unc-119(+)]. TY1::EGFP::3xFLAG tag inserted in frame at C-terminus of coding sequence by recombineering. Expression of transgene confirmed by GFP. References: Sarov M, et al. Nat Methods (2006) 10:839-44."

Proper citation: RRID:WB-STRAIN:WBStrain00047419 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047346

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: rcSi1 II; unc-119(ed3) III.
Notes: rcSi1 [mex-5p::rpt-1::mCherry + unc-119(+)] II. RPT-1::mCherry allows visualization of the proteasome in the germline. Reference: Sampuda KL, et al. BMC Cell Biol. 2017 Apr 19;18(1):18.

Proper citation: RRID:WB-STRAIN:WBStrain00047346 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047721

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: unc-119(ed3) III; czIs110.
Notes: czIs110 [mex-5p::GFP::KDEL::pie-1 3UTR + unc-119(+)]. GFP::KDEL is a marker of the luminal ER in the embryo. Reference: Lee et al., J Cell Biol. 2016 Sep 12;214(6):665-76.

Proper citation: RRID:WB-STRAIN:WBStrain00047721 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00047890

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: available
Source References: PMID:31242447
Synonyms: unc-119(ed4) III; syIs360.
Alternate IDs: CGC_PS7127
Notes: Batch Strain object requested via get_NS_ids.pl|"Generated from papers flagged positive during the last month for data type afp_strain/other_strain."|"syIs360 [ets-10p::GFP + unc-119(+)]. Dauer decision marker that indicates commitment to dauer formation in neurons and intestine. Reference: Shih, PY, et al., Dev Biol. 2019 Jun 23. pii: S0012-1606(18)30788-7."

Proper citation: RRID:WB-STRAIN:WBStrain00047890 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00054628

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: Cbr-unc-119(stIs20000) III; stIs20022.
Notes: Made_by: Waterston lab|"stIs20022 [Cbr-his-72::GFP::pie-1 3'UTR + Cbr-unc-119(+)]. C. briggsae with fluorescently labeled (GFP) histones. Reference: Zhou Z, et al. Genetics. 2010 Mar; 184(3): 853863. doi: 10.1534/genetics.109.110270 PMID: 20008572."|"[2023-10-27T18:59:54.666Z WBPerson51134] New Strain: C. briggsae. CGC_data_submission"

Proper citation: RRID:WB-STRAIN:WBStrain00054628 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00054699

Source Database: WormBase (WB)
Affected Genes: WBGene00000962(dhc-1)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00000962(dhc-1), WBGene00006843(unc-119)
Availability: unknown
Synonyms: dhc-1(lt45[dhc-1::GFP]) I; ltSi953 II; unc-119(ed3) III.
Notes: ltSi953 [mec-18p::vhhGFP4::ZIF-1::operon-linker::mKate2::tbb-2 3'UTR + Cbr-unc-119(+)] II. GFP tag inserted into the C-terminus of the endogenous dhc-1 locus using CRISPR-Cas9 engineering. Tissue-specific expression of GFP nanobody::ZIF-1 fusion promotes ubiquitylation and subsequent degradation of GFP-tagged dhc-1 protein in touch receptor neurons. Touch receptor neurons are red labeled with mKate2. Reference: Development. 2017 Jul 15;144(14):2694-2701. PMID: 28619826.

Proper citation: RRID:WB-STRAIN:WBStrain00054699 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00054684

Source Database: WormBase (WB)
Affected Genes: WBGene00001423(fib-1)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001423(fib-1), WBGene00006843(unc-119)
Availability: unknown
Synonyms: muIs252 II; unc-119(ed3) III; fib-1(mu498[wrmScarlet11::fib-1]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::linker::fib-1 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054684 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00054685

Source Database: WormBase (WB)
Affected Genes: WBGene00001877(his-3)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00001877(his-3), WBGene00006843(unc-119)
Availability: unknown
Synonyms: muIs252 II; unc-119(ed3) III; his-3(mu500[his-3::wrmScarlet11(x3)]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged his-3::wrmScarlet11(x3) generated via CRISPR/Cas9 insertion of three wrmScarlet11 tags into the endogenous his-3 locus in parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054685 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00054680

Source Database: WormBase (WB)
Affected Genes: WBGene00003230(mex-5)|WBGene00004896(smu-2)|WBGene00006843(unc-119)
Genomic Alteration: WBGene00003230(mex-5), WBGene00004896(smu-2), WBGene00006843(unc-119)
Availability: unknown
Synonyms: cer227[mex-5p::SpG(smu-2 introns) + unc-119(+)] II; unc-119(ed3) III.
Notes: Made_by: Dmytro Kukhtar|"Missense mutations D1135L and S1136W, G1218K and E1219Q, and R1335Q and T1337R were introduced on the Cas9 gene at EG9615 strain, to cause endogenous expression of the Cas9 variant SpG. SpG is efficient for CRISPR on NGN PAM sites. Reference: Vicencio J, et al. Nature Communication, 2022. May 12;13(1):2601. doi: 10.1038/s41467-022-30228-4."

Proper citation: RRID:WB-STRAIN:WBStrain00054680 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00054681

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)|WBGene00013025(vha-13)
Genomic Alteration: WBGene00006843(unc-119), WBGene00013025(vha-13)
Availability: unknown
Synonyms: muIs252 II; unc-119(ed3) III; vha-13(mu493[wrmScarlet11::vha-13]) V.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Homozygous viable. Endogenously-tagged wrmScarlet11::vha-13 generated via CRISPR/Cas9 insertion into parental strain CF4582. Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628

Proper citation: RRID:WB-STRAIN:WBStrain00054681 Copy   

•   Source: WormBase
  

http://www.wormbase.org/db/get?name=WBStrain00054757

Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Synonyms: aSi13 II; unc-119(ed3) III.
Notes: aSi13 [lox2272 + loxN 3' (delta)Cbr-unc-119(+) + 3' (delta)mNeonGreen::PEST] aSi14[lox2272 + loxP 3 (delta)HygR + 3 (delta)mScarlet-I::PEST]II. Unc. Strain contains a set of dual specialized safe harbor transgene landing pads for integration of promoters: one driving mScarlet and rescuing hygromycin resistance upon integration, the other driving mNeonGreen and rescuing the unc phenotype upon integration. Reference: Stevenson ZC, et al. bioRxiv 2022.10.30.514301; doi: https:

Proper citation: RRID:WB-STRAIN:WBStrain00054757 Copy   

•   Source: WormBase