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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janB-1/janB-1 (janB-1, III)
Affected Genes: JANB (janus B)
Source References: PMID:28305473
Notes: janus phenotype: partially reversed oral apparatus on the dorsal surface; two contractile-vacuole pore sets. Also the only temperature-sensitive janus mutant. There is very little expression of the janus phenotype at 28° C, strong expression at 39°.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01461 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Source References: PMID:15701804, PMID:16908532
Notes: homozygous, Neo cassette knockout before we knew there was HHT4
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01862 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT2; cy-r, IV)
Source References: PMID:10880474
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01985 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htay[∆123-388::neo3]/htay[∆123-388::neo3] (htay[∆123-388::neo3]; pm-r, ?)
Affected Genes: HTAY (TTHERM_01079200)
Genomic Alteration: Micronucleus: Neo3 ko of last 2/3 (AA123-388) of HTAY gene Macronucleus: Neo3 ko of last 2/3 (AA123-388) of HTAY gene
Source References: PMID:22426537
Notes: Knockout homokaryon of HTAY gene. Can mate with ∆HTAY(c2/3) 1-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02677 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Source References: PMID:16908532, PMID:15701804
Notes: homozygous, Neo cassette knockout before we knew there was HHT4
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01866 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT2; cy-r, IV)
Source References: PMID:10880474
Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01989 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding
Source References: PMID:10831613
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01905 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Notes: Collected by Paul Doerder on 7/30/2008 from the stream designated RedLkRn in PA (latitude 41.49, longitude -78.88)
Presence of micronucleus not determined. Mated with < 6 testers.
Proper citation: RRID:TSC_SD03095 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Notes: Collected by Paul Doerder on 7/29/2008 from the pond designated Beaver Meadows in PA (latitude 41.52, longitude -79.11)
Has a micronucleus. No mating, immature.
Proper citation: RRID:TSC_SD03093 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445A, E446A,E447A]; pm-r, mp-?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445A, E446A, E447A and neo replacing coding of some ATU1 genes
Source References: PMID:9037049, PMID:10831613
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pAAA derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E445A,E446A,E447A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02561 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; ts103/ts103 (CHX1; cy-s, tr, IV)
Proper citation: RRID:TSC_SD00140 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpD-2/mpD-2 (mpD-2, II)
Affected Genes: MPD (membranellar pattern D)
Notes: Formation of four or five well-formed membranelles in the oral apparatus at 39-40 degrees. Allelic to and phenotypically nearly identical to IA305 (mpD-1). Detailed phenotypic analysis was carried out on a different mpD-2/mpD-2 clone.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01231 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1
(mtt1-?[5’neo2,∆::BLT1, HAc], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BLT1with c-terminal HA replaces MTTi coding with neo2 upsteam in Mtt1 5’flanking region
Notes: CU428 somatic replacement with BLT-HA tag in the MTT1 locus, has Neo2 in 5’ flanking region of MTT1. Wild type BLT1 gene still present. This is a partial replacement. Cannot single-cell, only some cells express the HA tagged BLT when induced with Cd.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02562 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: lkn1-2/lkn1-2 (lkn1-2, III)
Affected Genes: LKN1 (low kinety number 1)
Source References: PMID:18658256
Notes: Reduced number of ciliary rows, accompanied by some irregularities in ciliary rows; cells tend to be short and squat. Expression is not temperature-sensitive. Allelic to lkn1-1(IA319): similar phenotype, fully penetrant but more weakly expressed. Fully penetrant at 29o C. Detailed phenotypic analysis was carried out on its sister clone, IA329.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01518 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 chimera protein hy3, H3(1-75) H3.3(76-90), H3(91-135); pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2(TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP tagged chimeric H3
Source References: PMID:16908532
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein hy3, H3(1-75) H3.3(76-90), H3(91-135) rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02692 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ? (Partial replacement
CDC2/cdc2-? [3’ neo3, ∆,(::GFP)]; pm-r with Cd+2, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: CDC2 gene has the 942 CDC2 5’ flanking region that includes the H1 dephosphorylation enriched region and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking. region.
Source References: PMID:17194754, PMID:15870266
Notes: XS67 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells. In CDC2 locus, coding region replaced by GFP coding. Neo3 cassette is in 3’ flanking region.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02572 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (WT; WT, VII)
Genomic Alteration: Macronucleus: RPL29::frame shift NEO
Source References: PMID:23426847
Notes: NEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03309 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 3L (CHX1; cy-s, VI)
Notes: Deletion on left arm of chromosome 3.
Proper citation: RRID:TSC_SD00954 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (ory-r, V)
Genomic Alteration: Micronucleus: btu1-1::btu1-1M350K/btu1-1::btu1-1M350K (homozygous) Macronucleus: btu1-1::btu1-1M350K (homozygous)
Proper citation: RRID:TSC_SD00281 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HTAZ ; MTT1/mtt1[5’neo2,∆::HTAZ(K4R)]; pm-r, VII)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Macronucleus: inserted HTAZ with K4R Into MTT1 locus and neo2 in 5’flanking of MTT1
Source References: PMID:12665578, PMID:11430834
Notes: Cell has wt H2A.Z in its locus, and a mutated H2A.Z RKKKKK copy in MTT1 locus, neo2 cassette is inserted at 5’ of MTT, complete replacement ?. Somatic transformation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02462 Copy
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