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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
http://www.wormbase.org/db/get?name=WBStrain00050661
Source Database: WormBase (WB)
Affected Genes: WBGene00004272(rab-8)
Genomic Alteration: WBGene00004272(rab-8)
Availability: unknown
Synonyms: rab-8(tm2526) I; muIs32 II.
Notes: muIs32 [mec-7p::GFP + lin-15(+)]. Homozygous viable. Reference: Kim KW, et al. Elife. 2018 Nov 21;7:e39756. doi: 10.7554/eLife.39756. PMID: 30461420
Proper citation: RRID:WB-STRAIN:WBStrain00050661 Copy
http://www.wormbase.org/db/get?name=WBStrain00050701
Source Database: WormBase (WB)
Availability: unknown
Synonyms: goeIs397.
Notes: goeIs397 [hsp-16.2p::cnc-10::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-10::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050701 Copy
http://www.wormbase.org/db/get?name=WBStrain00050667
Source Database: WormBase (WB)
Availability: unknown
Synonyms: bmdSi170 I.
Notes: bmdSi170 [loxN::eft-3p::his-58::GFP::3xHA] I. Superficially wild-type. bmdSi170 is a single-copy CRISPR/Cas9-engineered insertion of HIS-58 C-terminally tagged with non-codon-optimized GFP and driven by the ubiquitous eft-3 promoter. Reference: Azmi MA, et al. bioRxiv 2020.10.17.344069; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00050667 Copy
http://www.wormbase.org/db/get?name=WBStrain00050666
Source Database: WormBase (WB)
Availability: unknown
Synonyms: bmdSi141 I.
Notes: bmdSi141 [loxN::eft-3p::his-58::GFP] I. Slow growing. Maintain at 15-20C. bmdSi141 is a single-copy CRISPR/Cas9-engineered insertion of HIS-58 C-terminally tagged with codon-optimized GFP and driven by the ubiquitous eft-3 promoter. Reference: Azmi MA, et al. bioRxiv 2020.10.17.344069; doi: https:
Proper citation: RRID:WB-STRAIN:WBStrain00050666 Copy
http://www.wormbase.org/db/get?name=WBStrain00050665
Source Database: WormBase (WB)
Availability: unknown
Synonyms: bmdSi147 I.
Notes: bmdSi147 [loxN::rps-27p::DHB::2xmKate2::P2A::H2B::GFP] I. bmdSi147 is a single-copy CRISPR/Cas9-engineered insertion of a codon-optimized CDK sensor (amino acids 9941087 of human DNA Helicase B (DHB) fused to two copies of mKate2) co-expressed with his-58 (H2B) fused to GFP. Reference: Adikes RC, et al. Visualizing the metazoan proliferation-terminal differentiation decision in vivo. bioRxiv 2019.12.18.881888|"Made_by: Taylor N. Medwig-Kinney"
Proper citation: RRID:WB-STRAIN:WBStrain00050665 Copy
http://www.wormbase.org/db/get?name=WBStrain00050709
Source Database: WormBase (WB)
Availability: unknown
Synonyms: goeIs413.
Notes: goeIs413 [hsp-16.2p::cnc-9::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-9::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050709 Copy
http://www.wormbase.org/db/get?name=WBStrain00050708
Source Database: WormBase (WB)
Availability: unknown
Synonyms: goeIs412.
Notes: goeIs412 [hsp-16.2p::cnc-7::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-7::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050708 Copy
http://www.wormbase.org/db/get?name=WBStrain00050707
Source Database: WormBase (WB)
Availability: unknown
Synonyms: goeIs411.
Notes: goeIs411 [hsp-16.2p::cnc-4::SL2::mKate2::unc-54 3'UTR + unc-119(+)]. Over-expression of cnc-4::SL2::mKate2 after heat shock. Reference: Sinner MP, et al. Curr Biol. 2021 Feb 8;31(3):564-577.e12. PMID: 33259791|"Made_by: Florentin Masurat"
Proper citation: RRID:WB-STRAIN:WBStrain00050707 Copy
http://www.wormbase.org/db/get?name=WBStrain00050650
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00005016(sqt-1)|WBGene00077721(mir-1022)
Genomic Alteration: WBGene00003514(myo-2), WBGene00005016(sqt-1), WBGene00077721(mir-1022)
Availability: unknown
Synonyms: mir-1022(umn51[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) X.
Notes: Made_by: Julie Knott & Marcus Vargas|"mir-1022 pre-miRNA deletion allele in which mir-1022 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.]"
Proper citation: RRID:WB-STRAIN:WBStrain00050650 Copy
http://www.wormbase.org/db/get?name=WBStrain00050657
Source Database: WormBase (WB)
Affected Genes: WBGene00004443(rpl-29)
Genomic Alteration: WBGene00004443(rpl-29)
Availability: unknown
Synonyms: rpl-29(tm3555) IV.
Notes: Homozygous viable Reference: Noma et al Elife. 2017 Aug 2;6. pii: e26376. doi: 10.7554/eLife.26376.
Proper citation: RRID:WB-STRAIN:WBStrain00050657 Copy
http://www.wormbase.org/db/get?name=WBStrain00050654
Source Database: WormBase (WB)
Affected Genes: WBGene00004140(ebax-1)
Genomic Alteration: WBGene00004140(ebax-1)
Availability: unknown
Synonyms: ebax-1(ju699) IV; juEx1434.
Notes: juEx1434 [rgef-1p::ebax-1::GFP + rol-6(su1006)]. Pick Rollers to maintain. juEx1434 rescues Egl of pqn-55(ju699) mutants. Reference: Wang Z., et al. Neuron. 2013 Sep 4;79(5):903-16.
Proper citation: RRID:WB-STRAIN:WBStrain00050654 Copy
http://www.wormbase.org/db/get?name=WBStrain00050646
Source Database: WormBase (WB)
Affected Genes: WBGene00001067(dpy-5)|WBGene00001648(goa-1)|WBGene00006753(unc-14)
Genomic Alteration: WBGene00001067(dpy-5), WBGene00001648(goa-1), WBGene00006753(unc-14)
Availability: unknown
Synonyms: goa-1(n499)/tmC20 [unc-14(tmIs1219) dpy-5(tm9715)] I.
Notes: This strain is difficult and time consuming to maintain. Gives relatively few heterozygotes. Homozygous lethal mutation balanced by Dpy- and myo-2p::Venus-marked inversion. Heterozygotes are paralyzed Unc and Egl with relatively dim pharyngeal GFP (Venus) expression. Heterozygotes segregate heterozygous non-Dpy GFP+ paralyzed Unc and Egl, non-GFP embryonic lethal (homozygous n499), and Dpy with brighter GFP+ (tmC20 homozygous). Remove Dpy from plate to prevent them from taking over. Heterozygotes tend to stack up in parallel clumps. Populations can be enriched by transferring these clumps to new plates and allowing Dpy (tmC20 homozygotes) to crawl out into bacterial lawn, and then picking away Dpy or transferring the clump of Hets to another plate. Derived by balancing n499 from parental strain MT1102 over tmC20 from FX30179.
Proper citation: RRID:WB-STRAIN:WBStrain00050646 Copy
http://www.wormbase.org/db/get?name=WBStrain00050644
Source Database: WormBase (WB)
Affected Genes: WBGene00001073(dpy-11)|WBGene00006752(unc-13)|WBGene00006778(unc-42)
Genomic Alteration: WBGene00001073(dpy-11), WBGene00006752(unc-13), WBGene00006778(unc-42)
Availability: unknown
Synonyms: unc-13(e51)/hT1 I; dpy-11(e224)/hT1 [unc-42(e270) umnIs79] V.
Notes: Made_by: Julie Knott & Marcus Vargas|"umnIs79 [myo-2p::GFP + NeoR, I: 6284001] I. Pick wild-type GFP+ to maintain. Heterozygotes are wild-type GFP+, and segregate wild-type GFP+, DpyUnc, arrested hT1 homozygotes (GFP+), and dead eggs. Maintain by picking wild-type GFP+. Derived by insertion of myo-2p::GFP transgene into hT1 balancer in parental strain KR1037 using CRISPR/Cas9."
Proper citation: RRID:WB-STRAIN:WBStrain00050644 Copy
http://www.wormbase.org/db/get?name=WBStrain00050649
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00005016(sqt-1)|WBGene00050923(mir-1021)
Genomic Alteration: WBGene00003514(myo-2), WBGene00005016(sqt-1), WBGene00050923(mir-1021)
Availability: unknown
Synonyms: mir-1021(umn50[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) IV.
Notes: Made_by: Julie Knott & Marcus Vargas|"mir-1021 pre-miRNA deletion allele in which mir-1021 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.]"
Proper citation: RRID:WB-STRAIN:WBStrain00050649 Copy
http://www.wormbase.org/db/get?name=WBStrain00050648
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00005016(sqt-1)|WBGene00023457(mir-359)
Genomic Alteration: WBGene00003514(myo-2), WBGene00005016(sqt-1), WBGene00023457(mir-359)
Availability: unknown
Synonyms: mir-359(umn49[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) V.
Notes: Made_by: Julie Knott & Marcus Vargas|"mir-359 pre-miRNA deletion allele in which mir-359 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.]"
Proper citation: RRID:WB-STRAIN:WBStrain00050648 Copy
http://www.wormbase.org/db/get?name=WBStrain00050647
Source Database: WormBase (WB)
Affected Genes: WBGene00003514(myo-2)|WBGene00005016(sqt-1)|WBGene00077727(mir-1821)
Genomic Alteration: WBGene00003514(myo-2), WBGene00005016(sqt-1), WBGene00077727(mir-1821)
Availability: unknown
Synonyms: mir-1821(umn48[lox2272 myo-2p::wrmScarlet + lox511I sqt-1(d) hsp::CRE HygR LoX511I + Lox2272]) V.
Notes: Made_by: Julie Knott & Marcus Vargas|"mir-1821 pre-miRNA deletion allele in which mir-1821 pre-miRNA was replaced by myo-2p::wrmScarlet. Rollers. Generated in parental strain N2. [NOTE: Low levels of Cre activity can lead to excision of the SEC, causing the strain to lose the Roll phentoype. Pick Rollers to retain full transgene cassette.]"
Proper citation: RRID:WB-STRAIN:WBStrain00050647 Copy
http://www.wormbase.org/db/get?name=WBStrain00050639
Source Database: WormBase (WB)
Affected Genes: WBGene00006843(unc-119)
Genomic Alteration: WBGene00006843(unc-119)
Availability: unknown
Source References: PMID:39652010
Synonyms: muIs252 II; unc-119(ed3) III.
Notes: muIs252 [eft-3p::wrmScarlet1-10::unc-54 3'UTR + Cbr-unc-119(+)] II. Somatic expression of wrmScarlet1-10 (under the control of the eft-3 promoter and unc-54 3'UTR). Reference: Goudeau J, et al. Genetics. 2021 Apr 15;217(4):iyab014. doi: 10.1093/genetics/iyab014. PMID: 33693628
Proper citation: RRID:WB-STRAIN:WBStrain00050639 Copy
http://www.wormbase.org/db/get?name=WBStrain00050638
Source Database: WormBase (WB)
Affected Genes: WBGene00001078(dpy-19)|WBGene00001609(glp-1)|WBGene00003912(pal-1)
Genomic Alteration: WBGene00001078(dpy-19), WBGene00001609(glp-1), WBGene00003912(pal-1)
Availability: unknown
Synonyms: pal-1(ct224)/qC1 [dpy-19(e1259) glp-1(q339)] III; ctIs33.
Notes: ctIs33 [pal-1::GFP + rol-6(su1006)]. Heterozygotes are WT and segregate WT, DpySteriles and dead eggs. Pick wild-type heterozygotes to maintain. ct224 homozygotes show Nob phenotype: approximately 80% of homozygous embryos arrest at about the time of hatching with fairly normal anterior development but a severely deformed posterior with a variable knob-like shape; approximately 20% fail to enclose and do not hatch. ct224 is a 4.2kb deletion removing exon 1 through exon 6 of the pal-1 gene. ctIs33 carries a non-rescuing pal-1::GFP fusion containing ~7kb 5' of the SL1 splice site through part of exon 5 fused to GFP. GFP expression is primarily embryonic and limited to a few cells; not visible except at high magnification. Reference: Edgar LG, et al. Dev Biol. 2001 Jan 1;229(1):71-88.
Proper citation: RRID:WB-STRAIN:WBStrain00050638 Copy
http://www.wormbase.org/db/get?name=WBStrain00050730
Source Database: WormBase (WB)
Availability: unknown
Synonyms: vxIs824; vxIs591.
Notes: Made_by: Wisath Sae-Lee|"vxIs824 [rab-3p::ND18ApoE4::unc-54 3'UTR + myo-2p::mCherry::unc-54 3'UTR]. vxIs591 [tph-1p::GFP::unc-54 3'UTR]. Integrated transgene driving expression of human APOE4 throughout the nervous system; induces age-related neurodegeneration of HSNs and bag-of-worms in up to 60% of D3 adult worms. GFP expression in all serotonergic neurons. Reference: Sae-Lee et al. G3 (Bethesda) 2020 Aug 5;10(8):2851-2861. PMID: 32580938"
Proper citation: RRID:WB-STRAIN:WBStrain00050730 Copy
http://www.wormbase.org/db/get?name=WBStrain00050695
Source Database: WormBase (WB)
Affected Genes: WBGene00015145(arle-14)|WBGene00019883(met-2)
Genomic Alteration: WBGene00015145(arle-14), WBGene00019883(met-2)
Availability: unknown
Synonyms: arle-14(gw1623[GFP::TEV::3xFLAG::arle-14]) met-2(gw1419[met-2::FLAG::TEV::mCherry]) III.
Notes: Made_by: Colin Delaney|"Superficially wild-type. Endogenously tagged met-2 and arle-14 loci. MET-2::mCherry and GFP::ARLE-14 signal are detectable in all germline and somatic tissues. Reference: Delaney CE, et al. J Cell Biol. 2019 Mar 4;218(3):820-838. PMID: 30737265"
Proper citation: RRID:WB-STRAIN:WBStrain00050695 Copy
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