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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[T19A,T20A] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T19A, T20A
Source References: PMID:19822522
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-T(19/20)A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02100 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; chx2-1/chx2-1; mpr1-1/mpr1-1 (CHX1; CHX2; MPR1; cy-s, mp-s, III)
Source References: PMID:730051
Proper citation: RRID:TSC_SD00046 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[E445D,E446D]; pm-r, mp-?
)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1 with E445D, E446D and neo replacing coding of some ATU1 genes
Source References: PMID:9037049, PMID:10831613
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with pD5D6 derived from pTUB100E3-PvuII (linearized)where the ATU1 gene is marked with Hae III and Pvu II sites and E445D,E446D. No polyglycylation in this strain.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02585 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K4Q,K7Q,K10Q,K13Q,K16Q,K21R]; pm-r, ?)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutations K4Q, K7Q, K10Q, K13Q, K16Q, K21R
Source References: PMID:11430834, PMID:12665578
Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ mutated QQQQQR. Slow growth.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02073 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K115R]; htb2 [∆::neo2]; mp-r, II)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
Source References: PMID:19822522
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02471 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 5 (cnj3, pm-r, VII)
Proper citation: RRID:TSC_SD00294 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: MPR1/mpr1-1; GCP3/gcp3-1[Δ10-1201(::neo4, KanR2)] (MPR1/mpr1-1; GCP3/gcp3-1[Δ10-1201(::neo4, KanR2)]; mp-r, pm-r, ?)
Affected Genes: GCP3 (TTHERM_00112620)
Genomic Alteration: Micronucleus: GCP3 gene partially replaced by neo4-KanR2 cassette.
Notes: The knockout has been confirmed by 6-methylpurine and paromomycin resistance, but neither micronuclear nor macronuclear allele homozygosity has been verified. The neo4-KanR2 knockout cassette was generated by cloning fragments of the pNeo4 plasmid's neo4 cassette and the pUC4K plasmid's KanR2 cassette. The KanR2 gene was used for E. coli transformation and is not a selectable marker in Tetrahymena.
Submitted by Robert Coyne, J. Craig Venter Institute
Proper citation: RRID:TSC_SD01782 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 2 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01146 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; mpr1-1/mpr1-1; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];mpr1-1; pmr1-1; MAT2; cy-s, mp-r, pm-r, ?)
Source References: PMID:8725229, PMID:8601476
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01269 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1-1/pmr1-1; cnj3-1/cnj3-1 (cnj3-1; pmr1-1; cnj3, pm-r, ?)
Affected Genes: CNJ3
Source References: PMID:9299115
Proper citation: RRID:TSC_SD00212 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hho1[3’neo2, (T35E, S43E, S45E, T47E, T54E, T37K,A44K, T46K,V49K,V53K), GFPc]; pm-r, VII)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration: Macronucleus: Change Threonine 35, 47, 54 and serine 43, 45 to glutamic acid (charge patch) and T37K, A44K, T46K, V49K, V53K and GFP tag
Source References: PMID:10983971, PMID:11972045
Notes: Complete somatic tranformant of CU428 cells with pHHO5KE5 /neo construct. Phosphorylation sites 1- 5 of H1 changed to glutamic acid and 5 other sites changed to lysines,c-terminal GFP neo 2 cassette in 3’ flanking region for selection. See paper
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02478 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ift122[∆::neo3]/ift122[∆::neo3] (WT; pm-?, mp-?, ?)
Affected Genes: IFT122 (TTHERM_00694540)
Genomic Alteration: Micronucleus: Neo3 into coding of IFT122A
Source References: PMID:18211962, PMID:18199688
Notes: This is the non-star side of the IFT122 KO heterokaryon.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01787 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: WT (giw1-?[∆::neo3]; ∆p115, pm-r (neo3), II)
Affected Genes: p115, GIW1 (TTHERM_01276320)
Genomic Alteration: Macronucleus: KO of GIW1 with neo3
Source References: PMID:20211138
Notes: Somatic KO of p115, in B2086
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01823 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ift122[∆::neo3]/ift122[∆::neo3]; ift172[∆::neo3]/ift172[∆::neo3] (WT; pm-?, mp-?, ?)
Affected Genes: IFT122 (TTHERM_00694540), IFT172 (TTHERM_00089240)
Genomic Alteration: Micronucleus: Neo3 into coding of IFT122A and of IFT172
Source References: PMID:18199688, PMID:18211962
Notes: IFT122 and IFT172 double KO heterokaryon
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01789 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (MTT1/mtt1[5’neo2, ∆, 2HAn(::HTA.Y 1-258)]; pm-r, ?)
Affected Genes: HTAY (TTHERM_01079200)
Genomic Alteration: Macronucleus: Region of HTAY gene encoding AA 1 to 258 with two HA tags at n-terminus was inserted into the MTT1 gene.
Source References: PMID:22426537
Notes: Somatic transfomation of CU428xB2086 with pXS88. (MTT-neo2-2HA-HTA.Y n258) into MTT1 locus, Incomplete replacement
n258 means AA 1-258 of HTA.Y are present
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02884 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01156 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: lkn1-1/lkn1-1 (lkn1-1, V)
Affected Genes: LKN1 (low kinety number 1)
Source References: PMID:18658256
Notes: A reduced number of ciliary rows combined with a reduced fidelity in transmission of pre-existing row numbers, probably owing to interruptions and irregularities in the rows.
The phenotype is unconditional and penetrance is 100%. Cell mutiplication is unaffected.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01434 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (cnjB[∆?::neo3]; pm-r, VI)
Affected Genes: CnjB (TTHERM_01091290)
Genomic Alteration: Macronucleus: Neo3 replaces part of CnjB coding
Source References: PMID:19596782
Notes: somatic CnjB KO. Partial CnjB coding sequences were replaced by Neo3. Background: CU427.
Grow in 0.5 to 1.0 ug/ml CdCl2 and 100 ug/ml paromomycin.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02767 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆HHT2/HHF2;.∆HHT1;∆HHF1 homozygous heterkaryon (pm-r)
Affected Genes: HHT1 (TTHERM_00570560),HHT2 (TTHERM_00189180), HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170)
Notes: These are intermediate germlineKO heterokaryons not tested for somatic rescue. Final KO strains are #4 and#7. This is the star side.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02006 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; mpr1-1/mpr1-1; Nulli 3 (CHX1; mpr1-1; cy-s, mp-r, IV)
Proper citation: RRID:TSC_SD00108 Copy
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