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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
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Notes:
Proper citation: RRID:TSC_SD01177 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 4 (CHX1; cy-s, VI)
Affected Genes:
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Proper citation: RRID:TSC_SD01055 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 5 (CHX1; cy-s, III)
Affected Genes:
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Proper citation: RRID:TSC_SD00243 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT2; cy-r, IV)
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Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01972 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 2R [B or C3] (pmr1-1; cnj3, pm-r, VII)
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Proper citation: RRID:TSC_SD00402 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 1R (CHX1; pmr1-1; cy-s, pm-r, II)
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Notes: Large deletion. May be missing entire right arm of chromosome 1.
Proper citation: RRID:TSC_SD00769 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: rDNA [C3]/rDNA [C3]; mpr1-1/mpr1-1 (rDNA [B]; MPR1; mp-s, V)
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Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01618 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 2R (CHX1; cy-s, VI)
Affected Genes:
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Notes: Large deletion. May be missing entire right arm of chromosome 2.
Proper citation: RRID:TSC_SD00807 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hht2[∆::neo2]; pm-r, VII)
Affected Genes: HHT2 (TTHERM_00189180)
Genomic Alteration: Macronucleus: Neo2 cassettes KO HHT2
Availability:
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Notes: HHT2 knockout, neo 2 cassette.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02792 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (hta1[ S1V,K5R,K8R,K10R,k12R, K17R]; hta2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTA1 and HTA2 genes Macronucleus: mutated HTA1
Availability:
References:
Synonyms:
Notes: G4B1G10A x G4B3C1A. HTA.1 S1P plus all K changed to R in N-terminus used to rescue ∆HTA1∆HTA2 double knockout heterokaryon. Abolishes all acetylation.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02393 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (HTA1 (now HTAX) is WT but HTA2 (now HTA1) has neo2; pm-s?, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes Macronucleus: WT HTAX
Availability:
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Synonyms:
Notes: G4B1G10A x G4B3C1A. wt HTA1 (pXL53) rescue ∆HTA1∆HTA2 double knockout heterokaryon. Assort to pm-s? Mates with HTA1-D1M01
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02394 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (?HHT3/hht3[5’neo2,FLAGn]; pm-r, VII)
Affected Genes: HTT3 (TTHERM_00016170)
Genomic Alteration: Macronucleus: N-terminal His- tag of HHT3, not sure if complete replacement
Availability:
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Notes: Somatic replace HHT3 gene with HHT3-Flag sequence. Neo2 in 5’ flanking of HHT3
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02314 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 2 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
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Proper citation: RRID:TSC_SD00932 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo2]/ hhf1[∆::neo2] (mpr1-1; mp-r, ?)
Affected Genes: HHF1 (TTHERM_00498190)
Genomic Alteration: Micronucleus: HHF1 ko with neo2 cassette
Availability:
References:
Synonyms:
Notes: ∆H4I gl KO homozygous heterokaryon
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02322 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2] (HTAX/htax[∆neo2];hta1[∆::neo2]; HTB1/htb1[∆::neo2]; htb2[∆::neo2]; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 of HTAX, HTA1, HTB1, HTB2 Macronucleus: WT HTAX and HTB1, neo2 of HTA1, HTB2
Availability:
References:
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Notes: HTX1/HTB1 double wt rescue; Rescue of h∆∆HTA∆∆HTB-FBD1F6 and FBB1D3 with Dryl's.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02166 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/Chx1-1[C3]; MAT2/MAT3 (chx1-1; MAT3; cy-r, I)
Affected Genes:
Genomic Alteration:
Availability:
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Notes: Not useful for mapping loci on MIC chromosome 3R. One parent was missing this chromosome arm, so the progeny were not heterozygous.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01992 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mat1-3/mat1-3; serH-4/serH-4 (mat 1-3; serH-4, VII)
Affected Genes:
Genomic Alteration:
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Notes: Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01752 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cnjB[∆?::neo3]/ cnjB[∆?::neo3] (cnjB/cnjB
; wt)
Affected Genes: cnjB (TTHERM_01091290)
Genomic Alteration: Micronucleus: CnjB part of coding replaced with neo3
Availability:
References:
Synonyms:
Notes: Homozygous heterkaryon for ∆CnjB. Mates with ∆CnjB 2-1.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02049 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BTU2/btu2[∆,::H4-neo]; MPR1-1/mpr1-1 (BTU2/btu1[∆,::H4-neo]; MPR1-1/mpr1-1; mp-r, pm-r, ?)
Affected Genes: BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: H4-neo replaces BTU2 coding Macronucleus: H4-neo replaces BTU2 coding, not sure if complete
Availability:
References:
Synonyms:
Notes: Heterozygous germline transformant of BTU2 knockout. Derived from Cu428 X B2086 transformed w/ BHAB-2 (construct). When crossed to CU427, gave cyr and pmr progeny.
Can mate to B2KO-6. Created 12/29/97, in soybean until 4/98.
B2KO-6 mates with B2KO-1,2,3,4,5,7,8. No others mated.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01911 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; mpr1-1/mpr1-1 (pm-s, mp-r, wt HHT, ?)
Affected Genes: HHT1, HHT2, HHF1 (TTHERM_00570560, TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo3 KO of HHT1, HHT2/HHF2
Availability:
References:
Synonyms:
Notes: Germline homozygous heteokaryon for major H3 genes
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01881 Copy
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