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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=39128167
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Live Animals; Cryopreserved Sperm (as of 2020-09-23)
Alternate IDs: 39128167
Notes: This strain was generated by microinjecting YFP* (Venus): yellow fluorescent protein variant, which is inserted at downstream of Grp-promoter (4 kb) to Wistar rat zygotes. The number of inserted gene is approximately 100 copy. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_39128167 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=150429614
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 150429614
Notes: A full-length rat wild type Add3 cDNA obtained from an expression plasmid pCMV6-entry-Add3 purchased from Origene (Rockville, MD) was inserted in a sleeping beauty transposon vector. The expression of wild type ADD3 in the transposon vector was driven
by a CAG promoter. The construct was injected into the pronucleus ofoocytes collected from female FHH/EurMcwi rats along with SB100 transposase mRNA. A single-transgene insertion was identified on chromosome 10, which is located .64 kbp away from the protein shisa-6 homolog precursor at its 59 end and .360 kbp away from the phosphoinositide-interacting protein at the 3 prime end. Heterozygous founders were intercrossed to derive a homozygous transgenic line that was used for studies.
Proper citation: RRID:RGD_150429614 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38676447
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-17)
Alternate IDs: 38676447
Notes: This strain was established by Bac transgenic method (construct: Kazuhiro Yamakawa, phD) supported by C.B.S.Nresource (Dr. Kazuto Kobayashi, Dr. Yuchio Yanagawa) in 2014 and maintained at Jikei University School of Medicine. Background: Crlj:WI. Transgene: VGAT (Slc32a1: mouse, Kanamycin registance gene (E. coli), Cre (P1 phage), polyA signal (SV40 virus. Mouse BAC clone: RP23-392-P11). Cre recombinase is expressed under the control of mouse Slc32a1 (VGAT) promoter. This strain is used for GABAergic neuron-specific Cre-Lox site-specific recombination system. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38676447 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38676445
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-17)
Alternate IDs: 38676445
Notes: This strain was established by Bac transgenic method (construct: Kazuhiro Yamakawa, phD) supported by C.B.S.Nresource (Dr. Kazuto Kobayashi, Dr. Yuchio Yanagawa) in 2014 and maintained at Jikei University School of Medicine. Background: Crlj:WI. Transgene: VGAT (Slc32a1: mouse, Kanamycin registance gene (E. coli), Cre (P1 phage), polyA signal (SV40 virus. Mouse BAC clone: RP23-392-P11). Cre recombinase is expressed under the control of mouse Slc32a1 (VGAT) promoter. This strain is used for GABAergic neuron-specific Cre-Lox site-specific recombination system. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38676445 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=35668863
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 35668863
Notes: This strain was made by pronuclear injection into LEW/Crl embryos. The embryos were co-injected with DNA fragments containing the HLA-B*2705 human gene and the human beta-2-microglobulin gene. The strain carries 40 copies of HLA-B*2705 and 30 copies of human beta-2-microglobulin gene. Founder 21-3 was selected and carrier animals from this founder were mated and bred to homozygosity.
Proper citation: RRID:RGD_35668863 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=15045606
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 15045606
Notes: This transgenic strain line Tg12085 carries the dual-reporter LacZ/EGFP (enhanced green fluorescent protein) cloned into the rat Prnp vector (raPrnp) and the expression of transgenes were driven by rat Prnp promoter.
Proper citation: RRID:RGD_15045606 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=14981597
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 14981597
Notes: SD rats were microinjected with a linear 12 kb EcoRI-BamH1 fragment containing the coding sequence and promoter region of human SOD1 gene with G93A mutation. Originally created by John Kulik at Wyeth. Transgenic Model of SOD1 mediated Amyotrophic Lateral Sclerosis (ALS, Lou Gehrig's Disease). Came to Taconic in April 2002 for David Howland at Wyeth for distribution as an Emerging Model. Joint collaboration between Wyeth and The ALS Association. TACONIC
Proper citation: RRID:RGD_14981597 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=151356985
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 151356985
Notes: This LE transgenic rats were created by injecting the cfos-eGFP construct to pronuclei of fertilized eggs obtained from Long Evans females. The 5 prime end of the transgenic DNA construct contained mouse genomic cFos (indluding the promoter and all exon and intron sequences) was fused with eGFP (including poly(A) tail derived from the IRES (phosphorylated-internal ribosomal entry site) of the EGFP Clontech (Cambridge, UK) vector. A single founder animal was identified. All subsequent breeding used hemizygous cfos-GFP male rats paired with wild-type Long Evans female rats obtained from Charles River Laboratories. Rats were bred on Long-Evans background for 13 generations at NIDA IRP (Hope lab).
Proper citation: RRID:RGD_151356985 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38599204
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-15)
Alternate IDs: 38599204
Notes: The transgenic LE line 2(Back ground strain: Iar:Long-Evans (RGD:18337282) (Kumagai-shigeyasu Co.,Ltd)) carrying Transgene: ROSA26 (mouse ROSA26 BAC: RP23 244D9), and Channel rhodopsin fast receiver (ChRFR)(COP4*C167A) (Chlamydomonas reinhardtii) fused with YFP* (Venus). Copy number: 1, In this strain, COP4*C167A, modified photosensitivity cation channel, is expressed ubiquitously in the presence of exogenous Cre protein. The ChRFR(COP4*C167A), one of the channel rhodopsin (step-function opsin, SFO), opens under blue light and is closed under yellow light. The Venus (YFP*) is tagged with channel rhodopsin. There is no differences between line 1 and 2 except for copy number. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38599204 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38599203
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-15)
Alternate IDs: 38599203
Notes: The transgenic LE line 2(Back ground strain: Iar:Long-Evans (RGD:18337282) (Kumagai-shigeyasu Co.,Ltd)) carrying Transgene: ROSA26 (mouse ROSA26 BAC: RP23 244D9), and Channel rhodopsin fast receiver (ChRFR)(COP4*C167A) (Chlamydomonas reinhardtii) fused with YFP* (Venus). Copy number: 3, In this strain, COP4*C167A, modified photosensitivity cation channel, is expressed ubiquitously in the presence of exogenous Cre protein. The ChRFR(COP4*C167A), one of the channel rhodopsin (step-function opsin, SFO), opens under blue light and is closed under yellow light. The Venus (YFP*) is tagged with channel rhodopsin. There is no differences between line 1 and 2 except for copy number. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38599203 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=151356741
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 151356741
Notes: This transgenic strain was generated by microinjection into outbred Sprague-Dawley from Harlan Sprague-Dawley (Hsd:SD) fertilized eggs and carries simian virus T Antigen drived by the enhancer/promoter region of the mouse albumin gene.
Proper citation: RRID:RGD_151356741 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38599242
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-16)
Alternate IDs: 38599242
Notes: Mutant human amyloid beta (APP*) combined Thy1 promoter which is specifically expressed in neurons, was induced to ES cells from DA strain rats, and chimera rats obtained from the ES cells. The F1 rats were backcrossed with Crl:CD (SD) strain rats and F3 rats was deposited to NBRP. The expression levels of mutant human APP mRNA are difficult among Lines (12>1>14>6). National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38599242 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=39131285
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 39131285
Notes: This Cre recombinase reporter rat expressing mCherry was generated by backcrossing Sprague-Dawley to LE-Tg(DIO-mCherry)2Ottc (RGD:8693598).
The transgene contains Cre recombinase reporter rat expressing mCherry driven by the EF1 alpha promoter. Optogenetics and Transgenic Technology Core
Proper citation: RRID:RGD_39131285 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=38599244
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2020-09-16)
Alternate IDs: 38599244
Notes: Mutant human amyloid beta (APP*) combined Thy1 promoter which is specifically expressed in neurons, was induced to ES cells from DA strain rats, and chimera rats obtained from the ES cells. The F1 rats were backcrossed with Crl:CD (SD) strain rats and F3 rats was deposited to NBRP. The expression levels of mutant human APP mRNA are difficult among Lines (12>1>14>6). National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_38599244 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155663368
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 155663368
Notes: This transgenic model line 737 was made by injecting bacterial artificial chromosome (BAC) RNECO-180E22, into Crl:CD(SD) strain embryos. The BAC clone RNECO-180E22, a kind gift of Helen Skaletsky, is derived from Rattus norvegicus strain SHR chromosome Y.
Proper citation: RRID:RGD_155663368 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155260364
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2022-10-06)
Alternate IDs: 155260364
Notes: rat strain in which the rat Cyp3a1 gene (Cyp3a23-3a1, RGD:628626) was inactivated by insertion of a human CYP3A4 minigene (Cyp3A4*) and the linked rat Cyp3a2 locus was simultaneously mutated by CRISPR-mediated indel introduction. Strain contains Indel 8 at the Rat 3A2 locus (55 kb away), The CYP3A4 minigene included a CRISPR/Cas9 gene drive element to enable super-Mendelian inheritance. Strain contains and Active Genetic cassette which encodes a gRNA targeting the Rat 3A1 and 3A2 translation initiation site. ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSE Chenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan,Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin URL: Doi:10.1101/2022.08.30.505951 URL: Doi:10.1101/2022.08.30.505951
URL: URL: Doi:10.1101/2022.08.30.505951 RRRC
Proper citation: RRID:RGD_155260364 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155260365
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2022-10-06)
Alternate IDs: 155260365
Notes: This project entailed generation of a novel rat strain in which a P2A-Cas9-T2A-EGFP coding sequence was inserted at the stop codon of the rat Sycp1 gene for co-expression of Cas9 and EGFP under the control of the Sycp1 regulatory elements. Animals express Cas9 in the testis in the spermatogonial layer and in meiosis stages with 4N DNA content. Strain catalyzes high levels of Active Genetic Gene conversion when paired with certain Active Genetic cassettes. ROBUST AND EFFICIENT ACTIVE GENETICS GENE CONVERSION IN THE RAT AND MOUSE Chenyen Lai, Oscar Alvarez, Kristen Read, Don van Fossan,Christopher M Conner, Shannon Xaing-Ru Xu, Dale O. Cowley, Valentino Gantz, David R. Webb, Kurt Jarnagin
URL: Doi:10.1101/2022.08.30.505951 Donated to Rat Resource & Research Center
Proper citation: RRID:RGD_155260365 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=152977766
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Cryopreserved Sperm (as of 2022-07-06)
Alternate IDs: 152977766
Notes: Background strain: Iar:LE (Institute for Animal Reproduction) ( RGD:18337282). This strain was generated by injecting a modified BAC, in which a fusion protein created from the human IL2R alpha-subunit gene (IL2RA) and YFP* (enhanced yellow fluorescent protein,Venus) was inserted into the second exon of the rat Drd2 gene, into fertilized eggs of Long-Evans rats. National BioResource Project for the Rat in Japan
Proper citation: RRID:RGD_152977766 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=1357175
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 1357175
Notes: Wild type allele of Gimap5 from BN was microinjected into the pronuclei of fertilized eggs from a T cell lymphopenic congenic strain F344.DR-(D4Mit6-D4Mit24)
Proper citation: RRID:RGD_1357175 Copy
https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=1566459
Source Database: Rat Genome Database (RGD)
Genetic Background: transgenic
Availability: Unknown
Alternate IDs: 1566459
Notes: F344 embryos were microinjected with R26-EGFP trangene which comprises of 0.8 kb genomic sequence of ROSA 26 promoter fused to enhanced green fluorescent protein (EGFP).
Proper citation: RRID:RGD_1566459 Copy
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