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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (HTB1/htb1[∆::neo2]; htb2 [∆::neo2] assorted to pm-s so must have lost neo2; mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1
Source References: PMID:19822522
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2. Not mating type II,VI,VII.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02467 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2];hta1[∆::neo2]/hta1[∆::neo2] ; htb1[∆::neo2]/htb1[∆::neo2];htb2[∆::neo2]/htb2[∆::neo2 (mp-r?, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500), HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 replaces coding of HTAX, HTA1, HTB1, HTB2
Source References: PMID:19822522
Notes: HTX1/HTB1 quadruple KO heterokaryons;From mating of h∆∆HTA-F14A and h∆∆HTB-B3D3; made with B*VII. Not mating types II/VI/VII.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02627 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆::GTU1]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: GTU1 replaced by neo2 Macronucleus: GTU1 into MTT1 locus, partial replacement
Source References: PMID:12356864, PMID:16344310
Notes: In mac at GTU locus, GTU coding region is replaced by neo cassette. At metallothionein locus, two alleles a) MTT coding region, b) GTU coding region . Use the pTTMG construct (GTU coding driven by MTT promoter) to rescue GTU KO heterokaryon, is cadmium dependent. Use .25 to 1 ug/ml of CdCl2 to grow.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01416 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: atu1-1[Δ::neo1]/atu1-1[Δ::neo1]; mpr1-1/mpr1-1 or MPR1/MPR1 (atu1-1[Δ::neo1]/atu1[HAc]; pm-r, mp-?, ?)
Affected Genes: ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: neo replaces ATU1 coding Macronucleus: ATU1-HA or neo replacing coding of some ATU1 genes
Source References: PMID:10831613, PMID:9037049
Notes: It is a progeny of conjugants of AAK02.7XAAKO5.5 transformed with C-terminal HA tagged ATU1 gene.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02230 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: homozygous HMG C KO with BSR (mp-r)
Affected Genes: HMGB (TTHERM_00660180)
Notes: Obtained thru 1st round exclusion of ∆B∆C gl KO. Genotype confirmed by cross with 427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01387 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: act1[208::neo3]/act1[208::neo3] (pm-s , mp-?, ?)
Affected Genes: ACT1 (TTHERM_00190950)
Genomic Alteration: Micronucleus: ACT1 with neo3 inserted at Sfu1 site
Source References: PMID:16524910
Notes: ACT1∆ KO homozygous in mic, heterokaryon
From the Gorovsky lab, University of Rochester..
Proper citation: RRID:TSC_SD01797 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-?[E431D,E432D,E433D,E435D,E437D,E438D,E439D,E440D,E442D]; ATU1/atu1-1[duplicate ATU1 tail--AAA1]; pm-r, Original BTU2 is replaced by bsr1, BTU1 coding replaced by neo1, then transformed with BTU1 with (E431-442D) and Chimeric ATU1 gene, AAA1 (duplicate ATU C-tail))
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580), ATU1 (TTHERM_00558620)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU2 replaced by bsr. BTU1 partially replaced by neo1, some replaced by BTU1-D9 with (E431-442D). Some ATU1 replaced by ATU1 with duplicate C-tail
Source References: PMID:11864572, PMID:10831613, PMID:15254268
Notes: Progeny of DB6B x DB2A, co- transformed by constructs BTU1-D9 with (E431-442D) and construct of chimeric ATU1 gene, AAA1, duplicate ATU C-tail). See publications
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01833 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[S29A] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with S29A
Source References: PMID:19822522
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-S29A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02097 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3]; pmr1-1; MAT2; cy-s pm-r, ?)
Source References: PMID:8601476, PMID:8725229
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01291 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (ngoA[5’neo3, ∆::ngoA promoter 104bp,GFP], VII)
Affected Genes: ngoA (TTHERM_00655890)
Genomic Alteration: Macronucleus: GFP into NgoA locus with 104 bp of NgoA promoter. and neo3 upstream
Source References: PMID:15870266
Notes: XS 35 ngoA 5’-neo3-ngoA ch2p12-GFP-ngoA3’ Somatic transformant replacing WT ngoA gene with GFP coding driven by ngoA 5’ flanking 104 bp. Neo3 cassette is upstream of the 104 bp.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02703 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01102 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01107 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Nulli 2 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD00938 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 1L? [B or C3] (cnj3, pm-r, VII)
Proper citation: RRID:TSC_SD00386 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (cmp-r, III)
Notes: Cam-r lines grow more slowly than wild type.
For selection, make the drug up at 1 gram in 10 ml 95% ethanol, which makes a stock that is 250x, i.e. add 4ul drug/1 ml culture.
Proper citation: RRID:TSC_SD00025 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Uni 2R? [B or C3] (pmr1-1; cnj3, pm-r, VII)
Proper citation: RRID:TSC_SD00388 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Del 4 (CHX1; pmr1-1; cy-s, pm-r, II)
Proper citation: RRID:TSC_SD01116 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Nulli 1,2 (MPR1; cy-r, mp-s, VI)
Proper citation: RRID:TSC_SD00158 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr3-1[∆::NEO2] (Basal body organization defects., ?)
Affected Genes: SFR3 (TTHERM_00522410)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2]
Notes: sfr3 complete KO.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03305 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (VII)
Proper citation: RRID:TSC_SD00282 Copy
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