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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: homozygous HMG B KO with neo (mp-r)
Affected Genes: HMGB (TTHERM_00660180)
Notes: Obtained thru 1st round exclusion of ∆B∆C gl KO. Genotype confirmed by cross with 427
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01380 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K115R]; htb2 [∆::neo2]; mp-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 into coding of HTB1 and HTB2 Macronucleus: HTB1 with K115R, HTB2 KO with neo2
Source References: PMID:19822522
Notes: New batch, rescue of progeny h∆∆HTB86B4A6 and D2A2. Not mating type II,VI,VII
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02472 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: cen1-1[∆::NEO2] (cen1-1[∆::NEO2]::cen1-EF1; None., ?)
Affected Genes: CEN1 (TTHERM_00384910)
Genomic Alteration: Micronucleus: cen1-1[∆::NEO2] Macronucleus: cen1-1[∆::NEO2]::cen1-EF1
Source References: PMID:21562224
Notes: EF-hand mutant allele rescue of cen1∆ - D36A.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03284 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4] (drh1[∆?::neo4 ] Neo in 5’end of gene,some coding remains; pm-r, ?)
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: neo4 Macronucleus: neo4
Notes: Homozygous homokaryon KO of DRH1 with neo4 cassette. Mates with DRH1g∆∆ D3-11F.
Gene is Dicer Related Helicase, sequence is fromTGD 69.m00174. Mating cells stop at mic selection after MeiosisII Homozygous homokaryon. Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
Needs Cd for pm resistance.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02598 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; MPR1[C3]/MPR1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];mpr1-1; MPR1[C3]; MAT2; cy-s, mp-s, pm-r, ?)
Source References: PMID:8601476, PMID:8725229
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01267 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (Mac∆TWI1::neo3, twi1[∆::neo3]; mpr1-1; pm-r, mp-r, ?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Macronucleus: Coding region of TWI1 replaced by neo3
Source References: PMID:12297043
Notes: B2086 X CU428 were transformed with TWI1 KO construct (whole coding was replaced by MTT-neo-BTU) and endogenous TWI1 gene was pushed out. Somatic transformant
this strain can mate with this strain can mate with CU428,cu427, TWI1-KO2,-KO 4.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02111 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1[∆,::neo1]/btu1[∆,::neo1]; btu2[∆,::bsr1]/btu2[∆,::bsr1] (BTU1/btu1[∆,::neo1], BTU2; bsr-s, pm -r, ?)
Affected Genes: BTU1 (TTHERM_00348510) and BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: BTU1 coding replaced with neo1 cassette, BTU2 is blasticidin KO. Macronucleus: some BTU1::neo1 genes
Source References: PMID:10831613
Notes: Obtained from Jacek 11/1/99. Germline KO BTU1 neo1 cassette; BTU2 is blasticidin KO.
Mates with DB2A for heterokaryon rescue.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01943 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (mp-r, ?)
Affected Genes: HTA1 (TTHERM_00790790) + HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes
Source References: PMID:11430834, PMID:12665578
Notes: G115B5xG209C4. HTA1, HTA2 double germline knockout heterokaryon. Can mate with G4B1G6A, G4B1G10A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01390 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: CHX1[C3]/CHX1[C3]; pmr1-1/pmr1-1; MAT2/MAT2 (CHX1[C3];pmr1-1; MAT2; cy-s, pm-r, ?)
Source References: PMID:8725229, PMID:8601476
Notes: Resistance to 6-methylpurine was not scored for these panel members.
Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01273 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BTU11-428,BLT1428-473))], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pbBc-1 construct. BTU1(AA 1-428) with BLT at Cterminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02243 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[5’neo3,HAn]/twi11[5’neo3,HAn] (twi11[5’neo3,HAn]; pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
Notes: Homozygous HA-tagged TWI 11 homokaryon.HA coding sequence was inserted just after the start codon of T.WI11. neo3 cassette was inserted upstream of TWI11 coding.
mates with HA-TWI11 F2-1, F2-5
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02805 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: BLT1/ blt1-1[∆,neo3]; MPR1-?/ MPR1-? (BLT1; mpr1-1; mp-r, ?)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Micronucleus: Neo3 replaces BLT coding
Notes: BLT germline heterokaryon, heterozygous KO in mic, pm sensitive (120 ug/ml). Cd induced Neo cassette (1.2 ug/ml)--MTT promoter, Neo coding, BTU2 terminator. Construct pBLTMKO,
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02252 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[T19A,T20A] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with T19A, T20A
Source References: PMID:19822522
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-T(19/20)A.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02099 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1, vacA/vacA (mpr1, vacA; mp-r, vac-, II)
Source References: PMID:17248939
Notes: Submitted by Eduardo Orias, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01600 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: drh1[∆?::neo4]/drh1[∆?::neo4]
Affected Genes: DRH1 (TTHERM_00190830)
Genomic Alteration: Micronucleus: Neo4 into 5’ end of coding of DRH1
Notes: Neo4 KO cassette in DRH1 locus, homozygous KO in mic, mac assorted to pm-s. Two cell lines in row. Mate with A5, H9, and C10 cell lines. Homozygous heterokaryon.
Gene is expressed at low level in starve and log, peak expression at 4 hr of conjugation. Somatic KO is complete, when mated they arrest after Meiosis II. Homozygous homokaryon KOs do not grow well.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02142 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (eri[∆?::neo3]; pm-r, ?)
Affected Genes: ERI2 (TTHERM_00585450)
Genomic Alteration: Macronucleus: ∆eri2 complete ko
Notes: eri2 was expressed at low levels in growing, starved and conjugating cells. The coding sequences in MAC was replaced by Neo3.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02024 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo2, ∆, HAHAn(::PPO1)]; pm-r, VII)
Affected Genes: PPO1 (TTHERM_00865290, TTHERM_00865300)
Genomic Alteration: Macronucleus: PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
Source References: PMID:22426537
Notes: Used Xiayuan Song’s pXS 75 construct: neo 2 in 5 flank of MTT1 gene. 2 HA tags right after ATG. then PP50 coding sequence. PP50 is Ttherm. 00865300 into Ttherm 00865290 sequence. Need 100ug/ml pm in medium to keep the tagged gene present.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02821 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (III)
Notes: Collected by Paul Doerder on 7/13/2006 from the pond designated Gregg Lake in NH (latitude 43.04, longitude -71.99)
Presence of micronucleus not determined.
Proper citation: RRID:TSC_SD03083 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; Del 4 (CHX1; cy-s, VI)
Proper citation: RRID:TSC_SD01067 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; hht3[∆::neo2]/hht3[∆::neo2] (mpr1-1; pm-s, mp-r, ?)
Affected Genes: HHT (TTHERM_00570560, TTHERM_00189180, TTHERM_00016170)
Genomic Alteration: Micronucleus: Neo3 KO of major H3s and HHT3
Source References: PMID:15701804, PMID:16908532
Notes: homozygous, Neo cassette knockout before we knew there was HHT4
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD01863 Copy
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