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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: scr1-1/scri1-1 (scr1-1, II)
Affected Genes: SCR1 (screwy-1)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Ciliary rows helically wound around the cell. Expression is not temperature-sensitive. Note that this gene was published under the name TWI1, and later renamed SCR1 to avoid confusion with the piwi-homologue that had previously been named TWI1.
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01227 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; pmr1-1/pmr1-1; Nulli 5 (CHX1; pmr1-1; cy-s, pm-r, II)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01191 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (hta1[3’neo2]; pm-r, VII)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Macronucleus: HTA1 with neo2 cassette in 3’ flank
Availability:
References:
Synonyms:
Notes: Complete somatic transformant with HTA1-neo2 replacing the HTA1(HTAX) gene in mac of CU428. (Has~60 bp deleted in HTA1 3’flanking at about 500 bp downstream of TGA)
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02280 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gtu1[∆::neo2]/ gtu1[∆::neo2] (gtu1[∆::neo2]; MTT1/mtt1[∆, (::GTU1, 3HAc)]; pm-r, ?)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Micronucleus: Neo2 into GTU1 locus Macronucleus: GTU1 with 3HA tags at c-teminus into the MTT1 locus, partial replacement.
Availability:
References:
Synonyms:
Notes: pBTGHB HA rescued GTUKO 5x6 progeny. WT GTU with HA tag@c-terminus; driven by MTT1 promoter. Needs Cd to induce.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02047 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta1[∆::neo2]/hta1[∆::neo2]; hta2[∆::neo2]/ hta2[∆::neo2]; CHX1/CHX1;mpr1?/mpr1? (HTA2 has neo2; pm-r, ?)
Affected Genes: HTA1 (TTHERM_00790790), HTA2 (TTHERM_00316500)
Genomic Alteration: Micronucleus: Neo 2 knockout of HTAX and HTA1 genes Macronucleus: WT HTAX
Availability:
References:
Synonyms:
Notes: It is HTA1 WT somatic rescue strain of HTA1,HTA2 double knockout. Can mate with 1-5(12).
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02169 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (HTAZ/htaz[∆::neo2]; pm-r, mp-r, II)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding,
Availability:
References:
Synonyms:
Notes: New Batch of G304A1xG311A1 ∆HTAZ knockout heterokaryon. Rescued with HTAZ
Mating type not II,VI, or VII, Same with B3a.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02687 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (TBP1/tbp1[∆?::neo2]; pm-r, VII)
Affected Genes: TBP1 (TTHERM_00575350)
Genomic Alteration: Macronucleus: Partial Neo KO of TBP1
Availability:
References:
Synonyms:
Notes: Partial TATA-binding protein gene knockout with neo cassette,somatic.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02729 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (Circular permutation of HHO1 E5 charge patch, impossible to define in correct nomenclature, see paper.; pm-r, ?)
Affected Genes: HHO1 (TTHERM_00823720)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: E-M construct is used for somatic transformation
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02295 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (mlh1[243::neo2]; pm-r, ?)
Affected Genes: MLH1 (TTHERM_00471820)
Genomic Alteration: Macronucleus: Neo2 cassette inserted into coding of MLH1
Availability:
References:
Synonyms:
Notes: Transformation of CU428xB2086, complete knockout of mic histone linker gene with neo2 cassette inserted into Stu1 site. Paromomycin res. to 2 mg/ml Cell lines LH3 and LH6 can mate with each other.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02696 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: btu1-?[∆::neo1]/ btu1-?[∆::neo1]; btu2-?[∆::bsr1]/ btu2-?[∆::bsr1]; CHX1/CHX1; mpr1-1/mpr1-1 or MPR1/MPR1 (btu2-?[∆::bsr1]; btu1-?[∆::neo1]/ btu1-? [E431D,E432D,E433D,E435D]; pm-r, ?)
Affected Genes: BTU1 (TTHERM_00348510), BTU2 (TTHERM_00836580)
Genomic Alteration: Micronucleus: knockout of btu1 with neo
knockout of btu2 with bsr Macronucleus: BTU1 has only (E431, 432, 433, 435D) no WT
BTU2 is KO
Availability:
References:
Synonyms:
Notes: Progeny of DB6B x DB2A, transformed by BTU1-1235D mutation construct (E431, 432, 433, 435D)
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02212 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ? (Partial replacement CDC2/cdc2-? CDC2 gene 5’ flanking sequence of 480 bp with GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking.; pm-r with Cd+2, ?)
Affected Genes: CDC2 (TTHERM_01207660)
Genomic Alteration: Macronucleus: CDC2 gene has 5’ flanking sequence of 480 bp and GFP coding replacing CDC2 coding. Also neo3 in 3’ flanking.
Availability:
References:
Synonyms:
Notes: XS68 Somatic transformant obtained by shooting CU428 X B2086 exconjugant cells.WT gene CDC2 locus was partially replaced with GFP coding driven by CDC2 5’ flanking 480 bp region . Neo3 is in the CDC2 3’ flanking region.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02576 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htaz[∆::neo2]/htaz[∆::neo2]; mpr1-1/MPR1-1? (htaz[∆::neo2]/htaz[K4R,K7R,K10R,K13R,K16R]; pm-r)
Affected Genes: HTA3 (TTHERM_00143660)
Genomic Alteration: Micronucleus: Neo2 in HTAZ Macronucleus: HTAZ coding replaced with mutations K4R, K7R, K10R, K13R, K16R.
Availability:
References:
Synonyms:
Notes: G304A1xG311A1 ∆HTA3 knockout heterokaryon. Rescued with HTAZ mutated RRRRRK. Slow growth.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02456 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: WT (WT; WT, ?)
Affected Genes:
Genomic Alteration: Macronucleus: RPL29::frame shift NEO
Availability:
References:
Synonyms:
Notes: NEO coding sequence with two frameshift mutations in RPL29 vector (conferring resistance to Cyclohexamide, but remaining sensitive to paromomycin). The intention is this will provide the OLD MAC with a (defective) copy of the resistance gene, preventing its elimination in the NEW MAC after MIC transformation.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03307 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: WAG1/wag1[∆::neo3]; TWI11/twi11[neo3,HAn] (wag1[∆::neo3]; TWI11/HA tagged TWI11; pm-r, ?)
Affected Genes: WAG1 (TTHERM_00299879), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: heterozygous KO of WAG1 and TWI11, TWI1 also HA tagged Macronucleus: KO of WAG1 with neo3, HA tagged TWI11
Availability:
References:
Synonyms:
Notes: mates with ∆ WAG1 HAT11 W11
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02736 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: dcl1[∆?::neo3]/dcl1[∆?::neo3] (dcl1[∆?::neo3]/dcl1[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), DCL1 (TTHERM_00284230)
Genomic Alteration: Micronucleus: DCL1 replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Availability:
References:
Synonyms:
Notes: ∆DCL1 homokaryon transformed with Bsr-MTT1-CNA1-GFP.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01805 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1 (CHX1; cy-s, VI)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: Cycloheximide resistance functional heterokaryon.Regenerated micronucleus from CU427.2 by "star" mating. Non-star side maintained.
Proper citation: RRID:TSC_SD00715 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[∆?::neo3]/twi111[∆?::neo3] (twi11[∆?::neo3]/twi111[∆?::neo3]; MTT1/mtt1-?[5’ bsr1,∆,(::CNA1), GFPc]; bsr-r, pm-r, GFP tagged Cna1p, ?)
Affected Genes: CNA1 (TTHERM_00146340), TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11replaced by neo3 Macronucleus: CNA1 GFP tagged replaces MTT1 coding in MTT1 locus, bsr1 cassete in MTT1 5’
Availability:
References:
Synonyms:
Notes: ∆TWI11 homokaryon transformed with Bsr-MTT1-CNA1-GFP.
CNA1-GFP seq. was used to replace MTT1 coding seq. Bsr1 cassette was inserted at the 5’F seq. of MTT1 locus. The construct was transformed into mac.
Thaw cells without drugs, then grow them in 60-100ug/ml blasticidin, Use 0.5-1 ug/ml cadmium chloride to express and see the green centromere.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01809 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hhf1[∆::neo1]/hhf1[∆::neo1]; hht1[∆::neo2]/hht1[∆::neo2]; hhf2,hht2[∆::neo2]/hhf2,hht2[∆::neo2]; (Chimeric H3 with GFP tagged H3 chimera protein 4, H3(1-79) H3.3(80-135); pm-r, ?)
Affected Genes: HHF1 (TTHERM_00498190), HHF2 (TTHERM_00189170), HHT1 (TTHERM_00570560), HHT2 (TTHERM_00189180)
Genomic Alteration: Micronucleus: Neo KO of all major HHT genes and HHF genes Macronucleus: GFP tagged chimeric H3
Availability:
References:
Synonyms:
Notes: Germline heteokaryon Major H3 and H4 genes knocked out with neo cassette.It GFP tagged H3 chimera protein 4, H3(1-79) H3.3(80-135), rescue the progeny of major H3 H4 KO heterokaryons. can be mated with B2086.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02580 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: Nulli 2 (MPR1; mp-s, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD01014 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (TWI1::HA-TWI1-neo3, VII)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: CU428 was transformed with HA-TWI1-neo3 and endogenous TWI1 loci were completely replaced.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02068 Copy
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