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Integrated Animals is a virtual database currently indexing available animal strains and mutants from: AGSC (Ambystoma), BCBC (mice), BDSC (flies), European Xenopus Resource Center (frog), The National Xenopus Resource (frog), Xenopus Express (frog), CWRU Cystic Fibrosis Mouse Models (mice), DGGR (flies), FlyBase (flies), IMSR (mice), MGI (mice), MMRRC (mice), NSRRC (pig), RGD (rats), Sperm Stem Cell Libraries for Biological Research (rats), Tetrahymena Stock Center (Tetrahymena), WormBase (worms), XGSC (Xiphophorus), ZFIN (zebrafish), and ZIRC (zebrafish). Note, the IMSR data is linked, but users may need to re-execute the search if the top mouse is not returned properly.
Note: BCBC is no longer in service, so the links may not be functional.
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (GTU1/gtu1[3’neo2neo2,HAc], VII)
Affected Genes: GTU1 (TTHERM_00079520)
Genomic Alteration: Macronucleus: HA tag at c-terminus of GTU1 with neo2 in 3’ flanking region
Availability:
References:
Synonyms:
Notes: GTU (gamma tubulin) gene with c-terminal HA tag and two neo2 cassette inserted in 3’ flanking region. Partial replacement
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01413 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: RDR1/rdr1[∆::neo2] (?)
Affected Genes: RDR1 (TTHERM_00853150)
Genomic Alteration: Micronucleus: neo2 into RDR1
Availability:
References:
Synonyms:
Notes: Cross RDP1del X B2086. No other information
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02509 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: chx1-1/chx1-1; gal1-1/gal1-1; Nulli 2,3,4,5 (CHX1[A]; GAL1[A]; MPR1[A]; cy-s, gal-s, mp-s, III)
Affected Genes:
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00170 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (HA tagged HTAY and V5 tagged PPO1; cy-r, pm-r, VII)
Affected Genes: PPO1 (TTHERM_00865290)
Genomic Alteration: Macronucleus: HA tagged HTAY and PPO1 with n-terminal V5 tag
Availability:
References:
Synonyms:
Notes: HA2-HTAY cells transformed with V5-PP50 with the cycloheximide resistance cassette H4-rpL29-B upstream. Partial replacement . Cells are very sick, give yellow-green auto fluorescence with many vacuoles. Do give the correct ~36 kDa Western band for anti V5.
Grow with 15 ug/ml cy and 80 ug/ml pm.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02197 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: gal1-1/GAL1-1; chx1-1/CHX1-1 (gal1-1; chx1-1; gal-r; cy-r, VII)
Affected Genes:
Genomic Alteration:
Availability:
References:
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Notes: Generated by crossing SB1969 and SB210E. Progeny were selected as being cy-r and gal-r.
Submitted by Eileen Hamilton, University of California, Santa Barbara
Proper citation: RRID:TSC_SD01660 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi1[∆::neo3]/twi1[∆::neo3] (?)
Affected Genes: TWI1 (TTHERM_01161040)
Genomic Alteration: Micronucleus: Neo3 replaces coding of TWI1
Availability:
References:
Synonyms:
Notes: Homozygous heterokaryon of TWI1 gene with neo3, derived from B2086XCU428. Can mate with ∆TWI1hk-C4 and CU427.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02116 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: pmr1-1/pmr1-1; cnj5-1/cnj5-1 (cnj5-1; pmr1-1; cnj5, pm-r, II)
Affected Genes: CNJ5
Genomic Alteration:
Availability:
References:
Synonyms:
Notes:
Proper citation: RRID:TSC_SD00218 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-1[5’neo2,∆,HAc(::BTU11-428,BLT1428-473))], VII)
Affected Genes: BLT1 (TTHERM_01104960)
Genomic Alteration: Macronucleus: BTU1(AA 1-428) with BLT at C terminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstreamin MTT1 5’ region
Availability:
References:
Synonyms:
Notes: CU428 with BLT/BTU1 chimeric gene in MTT1 locus with HA tag. CU428 somatic transformant with pbBc-1 construct. BTU1(AA 1-428) with BLT at Cterminus (AA 428-473) followed by HA tag, inserted into the MTT1 locus. Neo2 upstream.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02242 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (btu1-? [∆2,3,4] ; BTU2/btu2-?[∆::neo1], ?)
Affected Genes: BTU (TTHERM_00348510)1, BTU2 (TTHERM_00836580)
Genomic Alteration: Macronucleus: BTU1 has the REI removed from the MREI at start of protien, BTU2 partically replaced by btu2::neo1
Availability:
References:
Synonyms:
Notes: Original strain B6-D(VB1 cells with BTU1,MREI has REI deleted but rest of BTU1 is WT, the VB1 mutation L350 to M350 is back to L350 so no longer hyper sensitive to taxol) This cell was somatically transformed. Need to check if all BTU1 is replaced.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02481 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: sfr13-1[∆::NEO2] (sfr13-1[∆::NEO2]; Reduced cell size, defective oral apparatus, fewer basal bodies, basal body stability defect, basal body separation and reorientation defect., ?)
Affected Genes: SFR13 (TTHERM_00522830)
Genomic Alteration: Micronucleus: cen2-1[∆::NEO2]
Availability:
References:
Synonyms:
Notes: sfr13 complete null.
From the Winey Lab at the University of Colorado Boulder.
Proper citation: RRID:TSC_SD03296 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: ∆BTU1/∆BTU1, ∆BTU2/∆BTU2, chx+/chx+,Mpr?/Mpr? (mp-r)
Affected Genes: BTU1 (TTHERM_00348510) + BTU2 (TTHERM_00836580)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: BTU1,BTU2 double germline knockout heterokaryon originally from Jacek Gaertig. ∆BTU1 by neo cassette, ∆BTU2 by blasticidin(BSR) cassette. It can mate with DB6B, received from Jacek Gaertig, 3/8/00.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02001 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hta2[∆::neo2]/hta2[∆::neo2]; mpr-1/mpr-? (mp-r, ?)
Affected Genes: HTA2 ( TTHERM_00316500 )
Genomic Alteration: Micronucleus: Neo 2 into HTA1, replaces coding
Availability:
References:
Synonyms:
Notes: CU428xB2086 HTA2 germline knockout heterokaryon transform with pQR17, remove all coding by neo. Can mate with G209A1 and G209C4.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01398 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: hht3[5’neo2,GFPc]/hht3[5’neo2,GFPc] (?)
Affected Genes: HHT3 (TTHERM_00016170)
Genomic Alteration: Micronucleus: GFP tagged HHT3
Availability:
References:
Synonyms:
Notes: non-star side of H3.3+GFP germline replacement heterokaryon.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD01950 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: twi11[5’neo3,HAn]/twi11[5’neo3,HAn] (twi11[5’neo3,HAn]; pm-r, ?)
Affected Genes: TWI11 (TTHERM_00144830)
Genomic Alteration: Micronucleus: TWI11-HA with neo3 in 5’ region Macronucleus: TWI11-HA with neo3 in 5’ region
Availability:
References:
Synonyms:
Notes: Homozygous HA-tagged TWI 11 homokaryon.HA coding sequence was inserted just after the start codon of TWI11. neo3 cassette was inserted upstream of TWI11 coding.
mates wtih F2-5,F2-8.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02801 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: disA-1/disA-1 (disA-1; disA, VII)
Affected Genes: DISA (disorganized A)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: disA-1 is a partially ts mutation that brings about disorganization of ciliary rows, without affecting the positioning of major cortical landmarks, or cytokinesis.
Proper citation: RRID:TSC_SD00629 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htb1[∆::neo2]/htb1[∆::neo2]; htb2 [∆::neo2]/htb2 [∆::neo2] (htb1[∆::neo2]/htb1[K4Q] ; htb2 [∆::neo2]; pm-r, ?)
Affected Genes: HTB1 (TTHERM_00633360), HTB2 (TTHERM_00283180)
Genomic Alteration: Micronucleus: neo2 KO of HTB1 and HTB2 coding Macronucleus: Mutated HTB1 with K4Q
Availability:
References:
Synonyms:
Notes: ∆∆HTB knockout heterokaryons rescued by htb1-K4Q.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02090 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: (?)
Affected Genes:
Genomic Alteration:
Availability:
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Notes: Collected by Paul Doerder on 7/24/2009 from the pond designated Willard Pond in NH (latitude 43.02, longitude -72.02)
Has a micronucleus. Mated with < 6 testers.
Proper citation: RRID:TSC_SD03107 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: mpr1-1/mpr1-1 (MTT1/mtt1-?[5’neo2, ∆, HAHAn(::PPO1)]; pm-r, VII)
Affected Genes: PPO1 (TTHERM_00865290, TTHERM_00865300)
Genomic Alteration: Macronucleus: PPO1 gene with 2 HA tags at n-terminus partially replace MTT1 coding, also neo2 in 5’ flanking region of MTT1.
Availability:
References:
Synonyms:
Notes: Used Xiayuan Song’s pXS 75 construct: neo 2 in 5 flank of MTT1 gene. 2 HA tags right after ATG. then PP50 coding sequence. PP50 is Ttherm. 00865300 into Ttherm 00865290 sequence. Need 100ug/ml pm in medium to keep the tagged gene present.
From the Gorovsky lab, University of Rochester. This strain has been cataloged by the Stock Center but not thawed.
Proper citation: RRID:TSC_SD02819 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: janB-1/janB-1; bcd1-1/bcd1-1 (janB-1; bcd1-1; janB, bcd1, II)
Affected Genes: JANB (janusB), BCD1 (broadened cortical domains1)
Genomic Alteration:
Availability:
References:
Synonyms:
Notes: A complex combination of additivity, enhancement of the janus phenotype, and suppression of one aspect of the bcd phenotype (broadened oral domains). Described in detail in Cole et al. (1988).
Submitted by Joseph Frankel, University of Iowa
Proper citation: RRID:TSC_SD01571 Copy
https://sites.wustl.edu/tetrahymena/finding-strains/
Source Database: TSC, Tetrahymena Stock Center
Genetic Background: htax[∆::neo2]/htax[∆::neo2]; hta1[∆::neo2]/hta1[∆::neo2 (HTAX/htax[∆::neo2]; hta1[∆::neo2]; pm-r, mp-r, ?)
Affected Genes: HTA1 (TTHERM_00790790)
Genomic Alteration: Micronucleus: KO of HTAX and HTA1 with neo2 Macronucleus: Complete KO of HTA1 with neo2, Partial replacement of HTAX with neo2
Availability:
References:
Synonyms:
Notes: New batch, rescue of h∆∆HTA-A1F14A1 and B1G6A These are KO of the major HTA genes HTAX and HTA1 in the mic with HTAX partial in the mac. Not mating type II,V1I or VII; same as HTA1-B1a.
From the Gorovsky lab, University of Rochester.
Proper citation: RRID:TSC_SD02387 Copy
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