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Tracing the lineage history of cells is key to answering diverse and fundamental questions in biology. Coupling of cell ancestry information with other molecular readouts represents an important goal in the field. Here, we describe the CRISPR array repair lineage tracing (CARLIN) mouse line and corresponding analysis tools that can be used to simultaneously interrogate the lineage and transcriptomic information of single cells in vivo. This model exploits CRISPR technology to generate up to 44,000 transcribed barcodes in an inducible fashion at any point during development or adulthood, is compatible with sequential barcoding, and is fully genetically defined. We have used CARLIN to identify intrinsic biases in the activity of fetal liver hematopoietic stem cell (HSC) clones and to uncover a previously unappreciated clonal bottleneck in the response of HSCs to injury. CARLIN also allows the unbiased identification of transcriptional signatures associated with HSC activity without cell sorting.
Pubmed ID: 32413320
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This monoclonal targets CD11b
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View all literature mentionsThis monoclonal targets CD3
View all literature mentionsThis monoclonal targets CD49f (Integrin alpha 6)
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View all literature mentionsThis monoclonal targets Ly-6G/Ly-6C
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View all literature mentionsThis monoclonal targets CD11b
View all literature mentionsThis monoclonal targets Ly-6G
View all literature mentionsThis monoclonal targets TER-119
View all literature mentionsThis monoclonal targets CD41
View all literature mentionsThis monoclonal targets CD45R (B220)
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Mus musculus with name B6;129S4-Gt(ROSA)26Sortm1(rtTA*M2)Jae Col1a1
